Sizinikezele ekulawuleni okuhle kakhulu kanye nenkampani yabathengi ecabangelayo, abasebenzi bethu abanokuhlangenwe nakho esisebenzisana nabo bavame ukutholakala ukuze baxoxe ngezimfuno zakho futhi benze okuthile okugcwele komthengi kujabulele I-Professional Design China CE Igunyazwe 32 Well Plate Magnetic Bead Method DNA Nucleic Acid Isolation Kit Yokutholwa Kwegciwane, Ukwamukela izinhlangano ezithakaselekayo ukubambisana nathi, sibheka phambili ngokuhlanganyela umhlaba wonke ukuze sithole impumelelo.
Sizinikezele ekulawuleni okuhle kakhulu kanye nenkampani yabathengi ecabangelayo, abasebenzi bethu abanokuhlangenwe nakho esisebenzisana nabo bavame ukutholakala ukuze baxoxe ngezidingo zakho futhi benze okuthile okujabulisayo komthengiI-China Nucleic Acid Extraction, Ikhithi yokukhipha i-Rna, Ukwethembeka yinto ehamba phambili, futhi isevisi iwukubaluleka.Sithembisa ukuthi sinekhono lokuhlinzeka ngekhwalithi enhle kakhulu nezimpahla zamanani aphusile kumakhasimende.Nathi, ukuphepha kwakho kuqinisekisiwe.

Imininingwane

50 Preps, 100 Preps, 250 Preps

Le khithi isebenzisa ikholomu ye-DNA kuphela engabopha ngokuqondile i-DNA, i-Foregene protease kanye nesistimu ye-buffer eyingqayizivele.I-DNA yezinga eliphezulu ye-genomic ingakhishwa kumaseli akhulisiwe ahlukahlukene kanye nezicubu zezilwane kungakapheli imizuzu engama-30 kuye kwengama-50.

Ulwelwesi lwejeli ye-silica ye-DNA kuphela esetshenziswa kukholamu yokuphotha iyinto entsha eyingqayizivele ye-Foregene, engabopha ngempumelelo nangokukhethekile ku-DNA, futhi ikhulise ukukhishwa kwe-RNA, amaprotheni angcolile, ama-ion nezinye izinhlanganisela eziphilayo kumaseli.I-5-80μg yekhwalithi ephezulu ye-genomic DNA ingahlanzwa kusuka ku-10-50mg izicubu.

Izingxenye zekhithi

Izici&izinzuzo

■ Akukho ukungcoliswa kwe-RNase: Ikholomu Ye-DNA Kuphela enikezwe kukhithi yenza kube nokwenzeka ukususa i-RNA ku-DNA ye-genomic ngaphandle kwe-RNase eyengeziwe phakathi nokuhlolwa, ngaleyo ndlela ivimbele ilabhorethri ukuthi ingangcoliswa i-RNase yangaphandle.

■ Isivinini esisheshayo: I-Foregene Protease inomsebenzi ophezulu kunama-protease afanayo futhi igaya amasampula ezicubu ngokushesha.

■ Okulula : ukusebenza kwe-genomic DNA extraction kungaqedwa ngemizuzu engama-50.

■ Ilula: I-centrifugation yenziwa ekamelweni lokushisa, akukho 4℃ lokushisa eliphansi centrifugation noma ethanol precipitation of DNA.

■ Ukuphepha: asikho i-reagent ephilayo edingekayo.

■ Ikhwalithi ephezulu: I-DNA ye-genomic ekhishiwe inezicucu ezinkulu, ayikho i-RNA, ayikho i-RNase, nokuqukethwe kwe-ion ephansi kakhulu, kungahlangabezana nezidingo zokuhlola okuhlukahlukene.

Isicelo sekhithi

Ilungele ukukhishwa kanye nokuhlanzwa kwe-DNA ye-genomic kusuka kumaseli akhulisiwe amasha noma afriziwe kanye nezicubu zezilwane.

Ukugeleza komsebenzi

Umdwebo

Isitoreji kanye Nempilo yeshelufu

Ikhithi ingagcinwa izinyanga eziyi-12 ekamelweni lokushisa (15–25 ℃) kanye no-2–8 ℃ isikhathi eside. Njengoba inikezelwe ekulawuleni okuhle kakhulu nenkampani yabathengi ecabangelayo, amalungu ethu anolwazi lwabasebenzi esisebenzisana nabo bavame ukutholakala ukuze baxoxe ngezidingo zakho futhi benze okuthile okujabulisayo komthengi okugcwele kwe-Professional Design China CE Igunyazwe 32 Well Plate Magnetic Bead Methoict Acid DNA s ukubambisana nathi, sibheka phambili ekutholeni ithuba lokusebenza nezinhlangano emhlabeni wonke ukuze sikhule ngokuhlanganyela kanye nempumelelo efanayo.
Idizayini yobungcwetiI-China Nucleic Acid Extraction, Ikhithi yokukhipha i-Rna, Ukwethembeka yinto ehamba phambili, futhi isevisi iwukubaluleka.Sithembisa ukuthi sinekhono lokuhlinzeka ngekhwalithi enhle kakhulu nezimpahla zamanani aphusile kumakhasimende.Nathi, ukuphepha kwakho kuqinisekisiwe.