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Isitshalo Esiphelele se-RNA Isolate Kit
Ikhithi isebenzisa ikholomu ye-spin nefomula eyenziwe yi-Foregene, engakhipha kahle i-RNA ephezulu kakhulu nekhwalithi ephezulu kuzicubu zezitshalo ezahlukahlukene ezinokuqukethwe okuphansi kwe-polysaccharides kanye ne-polyphenols. Ngamasampula ezitshalo ane-polysaccharides ephezulu noma okuqukethwe kwe-polyphenols, kunconywa ukuthi kusetshenziswe iPlant Total RNA Isolate Plus Kit ukuthola imiphumela engcono yokukhipha i-RNA. Ikhithi inikeza ikholomu yokuhlanza i-DNA engasusa kalula i-genomic DNA kusuka ku-supernatant ne-lysate yezicubu. Ikholomu ye-RNA kuphela ingabopha ngempumelelo i-RNA. Ikhithi ingacubungula inani elikhulu lamasampula ngasikhathi sinye.
Uhlelo lonke alunayo i-RNase, ngakho-ke i-RNA ehlanzekile ngeke yehliswe. IBuffer PRW1 neBuffer PRW2 ingaqinisekisa ukuthi i-RNA etholakele ayingcoliswanga amaprotheni, iDNA, ions, kanye nezinhlanganisela zemvelo.
