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I-Zebra Fish Direct PCR Kit
Leli thuluzi lisebenzisa uhlelo oluyingqayizivele lwe-lysis buffer ukukhipha ngokushesha i-genomic DNA kusuka kuma-zebrafish nakwezinye izicubu zezinhlanzi zasemanzini angenasawoti, amaphiko omsila noma amasampula amaqanda ezinhlanzi ekuphenduleni kwe-PCR, ngakho-ke kufaneleke kakhulu ukuhlolwa kwezakhi zofuzo ezinkulu. Inqubo yokukhipha i-genomic DNA kusuka kusigaxa se-lysis iphothulwe kungakapheli imizuzu eyi-10-30 ku-65 ° C. Azikho ezinye izinqubo ezifana namaprotheni nokususwa kwe-RNA okudingekayo, futhi umkhondo we-DNA okhishiwe ungasetshenziswa njengesifanekiso sokuphendula kwe-PCR.
I-2 × PCR Easy TM Mix inokubekezelelana okuqinile kuma-PCR reaction inhibitors, futhi ingasebenzisa i-lysate yesampula ezohlolwa njengesifanekiso sokukhulisa okusebenzayo futhi okuqondile. Le reagent iqukethe i-ForegeneD-Taq DNAPolymerase, i-dNTPs, i-MgCl2, i-reaction buffer, i-PCR optimizer kanye ne-stabilizer. Isetshenziswe ngokubambisana ne-lysis buffer ingathola amasampula ngokushesha futhi kalula, futhi inezici zokuzwela okuphezulu, ukucaciswa okuqinile nokuqina okuhle.
I-D-Taq DNA polymerase iyi-DNA polymerase eyenziwe ngokukhethekile yi-Foregene yokuphendula ngqo kwe-PCR. I-D-Taq DNA polymerase inokubekezelelana okuqinile kuma-PCR reaction inhibitors ahlukahlukene, futhi ingakhulisa kahle inani lokulandela ngomkhondo le-DNA ezinhlelweni ezahlukahlukene zokuphendula eziyinkimbinkimbi, futhi ijubane lokukhulisa lingafinyelela ku-2Kb / min, elifanele ikakhulukazi ukusabela okuqondile kwe-PCR Direct.
