Isilwane

  • Animal Tissue Direct PCR kit

    Ikhithi ye-PCR Direct Tissue Direct

    Leli thuluzi lisebenzisa uhlelo oluyingqayizivele lwe-lysis buffer ukukhipha ngokushesha i-genomic DNA kusuka kumasampula wezicubu zezilwane ekuphenduleni kwe-PCR, ngakho-ke ilungele ikakhulukazi ukuhlolwa okukhulu kofuzo.

    Inqubo yokukhipha i-genomic DNA kusuka kusigaxa se-lysis iphothulwe kungakapheli imizuzu eyi-10-30 ku-65°C. Azikho ezinye izinqubo ezifana namaprotheni nokususwa kwe-RNA okudingekayo, futhi umkhondo we-DNA okhishiwe ungasetshenziswa njengesifanekiso sokuphendula kwe-PCR.

    2× PCR EasyTM Imix inekubekezelela okuqinile kuma-PCR reaction inhibitors, futhi ingasebenzisa i-lysate yesampula ukuze ihlolwe njengesifanekiso sokukhulisa okusebenzayo futhi okuqondile. Le reagent iqukethe i-Foregene D-Taq DNAPolymerase, dNTPs, MgCl2, i-reaction buffer, i-PCR optimizer kanye ne-stabilizer.

    I-D-Taq DNA polymerase iyi-DNA polymerase eyenziwe ngokukhethekile yi-Foregene yokuphendula ngqo kwe-PCR. I-D-Taq DNA polymerase inokubekezelelana okuqinile kuma-PCR reaction inhibitors ahlukahlukene, futhi ingakhulisa kahle inani lokulandela ngomkhondo le-DNA ezinhlelweni ezahlukahlukene zokuphendula eziyinkimbinkimbi, futhi ijubane lokukhulisa lingafinyelela ku-2Kb / min, elifanele ikakhulukazi ukusabela okuqondile kwe-PCR Direct.

  • Mouse Tail SuperDirect PCR Kit

    Igundane Umsila SuperDirect PCR Kit

    Leli thuluzi lisebenzisa uhlelo oluyingqayizivele lwe-lysis buffer ukukhipha ngokushesha i-genomic DNA kusuka kumasampula wegundane nezindlebe zegundane ekuphenduleni kwe-PCR, ngakho-ke ilungele ikakhulukazi ukuhlolwa okukhulu kofuzo.

    Inqubo yokukhipha i-genomic DNA kusuka kusigaxa se-lysis isiqediwe kungakapheli imizuzu eyi-10-30 ekamelweni lokushisa (20-25 ° C). Akukho ukushisa okudingekayo, futhi ezinye izinqubo ezinjenge-protein ne-RNA ukususwa akudingeki. Inani elilandelwayo le-DNA lingasetshenziswa njengesifanekiso sokuphendula kwe-PCR.

    I-2 × M-PCR Easy TM Mix inokubekezelelana okuqinile kuma-PCR reaction inhibitors, futhi ingasebenzisa i-lysate yesampula ezohlolwa njengesifanekiso sokukhulisa okusebenzayo futhi okuqondile. Le reagent iqukethe i-Foregene D-Taq DNAPolymerase, i-dNTPs, i-MgCl2, i-reaction buffer, i-PCR optimizer kanye ne-stabilizer.

    I-D-Taq DNA polymerase iyi-DNA polymerase eyenziwe ngokukhethekile yi-Foregene yokuphendula ngqo kwe-PCR. I-D-Taq DNA polymerase inokubekezelelana okuqinile kuma-PCR reaction inhibitors ahlukahlukene, futhi ingakhulisa kahle inani lokulandela ngomkhondo le-DNA ezinhlelweni ezahlukahlukene zokuphendula eziyinkimbinkimbi, futhi ijubane lokukhulisa lingafinyelela ku-2Kb / min, elifanele ikakhulukazi ukusabela okuqondile kwe-PCR Direct.

  • Animal Tissue Direct PCR kit-UNG

    I-Animal Tissue Direct PCR kit-UNG

    Leli thuluzi lisebenzisa uhlelo oluyingqayizivele lwe-lysis buffer ukukhipha ngokushesha i-genomic DNA kusuka kumasampula wezicubu zezilwane ekuphenduleni kwe-PCR, ngakho-ke ilungele ikakhulukazi ukuhlolwa okukhulu kofuzo.

    Inqubo yokukhipha i-genomic DNA kusuka kusigaxa se-lysis iphothulwe kungakapheli imizuzu eyi-10-30 ku-65°C. Azikho ezinye izinqubo ezifana namaprotheni nokususwa kwe-RNA okudingekayo, futhi umkhondo we-DNA okhishiwe ungasetshenziswa njengethempulethi yokuphendula kwe-PCR.

    2×PCR EasyTM IMix (UNG) isebenzisa i-DUTP esikhundleni se-dTTP ngesisekelo se-2×PCR EasyTM Hlanganisa, bese ungeza i-enzyme ye-UNG (i-Uracil-N-glycosylase) engalulaza isifanekiso esine-DUTP ngasikhathi sinye. Ngaphambi kokuphendula kwe-PCR, i-enzyme ye-UNG isetshenziselwa ukwehlisa umkhiqizo we-PCR oqukethe i-uracil. I-enzyme ye-UNG ngeke ibe nomthelela kuthempulethi engenayo i-uracil, ngaleyo ndlela iqinisekise ukucaciswa nokunemba kokukhulisa nokuvimbela ukuthi kungenzeka ukungcoliswa kwemikhiqizo ye-PCR ngesikhathi sokuhlolwa okukhulu kofuzo.

    I-D-Taq DNA polymerase iyi-DNA polymerase eyenziwe ngokukhethekile yi-Foregene yokuphendula ngqo kwe-PCR. I-D-Taq DNA polymerase inokubekezelelana okuqinile kuma-PCR reaction inhibitors ahlukahlukene, futhi ingakhulisa kahle inani lokulandela ngomkhondo le-DNA ezinhlelweni ezahlukahlukene zokuphendula eziyinkimbinkimbi, futhi ijubane lokukhulisa lingafinyelela ku-2Kb / min, elifanele ikakhulukazi ukusabela okuqondile kwe-PCR Direct.

  • Mouse Tail Direct PCR Kit

    Umsila weMouse oqondile we-PCR Kit

    Leli kit lisebenzisa uhlelo oluyingqayizivele lwe-lysis buffer ukukhipha ngokushesha i-genomic DNA kusuka emisileni yegundane, izindlebe, imisipha namanye amasampula wezicubu zokuphendula kwe-PCR, ngakho-ke ilungele ikakhulukazi ukuhlolwa okukhulu kofuzo.

    Inqubo yokukhipha i-genomic DNA kusuka kusigaxa se-lysis iphothulwe kungakapheli imizuzu eyi-10-30 ku-65 ° C. Azikho ezinye izinqubo ezifana namaprotheni nokususwa kwe-RNA okudingekayo, futhi umkhondo we-DNA okhishiwe ungasetshenziswa njengesifanekiso sokuphendula kwe-PCR.

    I-2 × M1-PCR Easy TM Mix inokubekezelelana okuqinile kuma-PCR reaction inhibitors, futhi ingasebenzisa i-lysate yesampula ezohlolwa njengesifanekiso sokukhulisa okusebenzayo futhi okuqondile. Le reagent iqukethe i-Foregene D-Taq DNAPolymerase, i-dNTPs, i-MgCl2, i-reaction buffer, i-PCR optimizer kanye ne-stabilizer.

    I-D-Taq DNA polymerase iyi-DNA polymerase eyenziwe ngokukhethekile yi-Foregene yokuphendula ngqo kwe-PCR. I-D-Taq DNA polymerase inokubekezelelana okuqinile kuma-PCR reaction inhibitors ahlukahlukene, futhi ingakhulisa kahle inani lokulandela ngomkhondo le-DNA ezinhlelweni ezahlukahlukene zokuphendula eziyinkimbinkimbi, futhi ijubane lokukhulisa lingafinyelela ku-2Kb / min, elifanele ikakhulukazi ukusabela okuqondile kwe-PCR Direct.

  • Mouse Tail Direct PCR Kit-UNG

    Umsila weMouse oqondile we-PCR Kit-UNG

    Leli kit lisebenzisa uhlelo oluyingqayizivele lwe-lysis buffer ukukhipha ngokushesha i-genomic DNA kusuka emisileni yegundane, izindlebe, imisipha namanye amasampula wezicubu zokuphendula kwe-PCR, ngakho-ke ilungele ikakhulukazi ukuhlolwa okukhulu kofuzo.

    Inqubo yokukhipha i-genomic DNA kusuka kusigaxa se-lysis iphothulwe kungakapheli imizuzu eyi-10-30 ku-65 ° C. Azikho ezinye izinqubo ezifana namaprotheni nokususwa kwe-RNA okudingekayo, futhi umkhondo we-DNA okhishiwe ungasetshenziswa njengesifanekiso sokuphendula kwe-PCR.

    I-2 × M1-PCR Easy TM Mix (UNG) isebenzisa i-DUTP esikhundleni se-dTTP ngesisekelo se-2 × M1-PCR Easy TM Mix (UNG), futhi ingeza i-enzyme ye-UNG (i-Uracil-N-glycosylase) engehlisa isifanekiso esine-DUTP Ngisikhathi esifanayo. Ngaphambi kokuphendula kwe-PCR, i-enzyme ye-UNG isetshenziselwa ukwehlisa umkhiqizo we-PCR oqukethe i-uracil. I-enzyme ye-UNG ngeke ibe nomthelela kuthempulethi engenayo i-uracil, ngaleyo ndlela iqinisekise ukucaciswa nokunemba kokukhulisa nokuvimbela ukuthi kungenzeka ukuhlolwa okukhulu kofuzo. Ukungcoliswa kwemikhiqizo ye-PCR kwenzeke.

    I-D-Taq DNA polymerase iyi-DNA polymerase eyenziwe ngokukhethekile yi-Foregene yokuphendula ngqo kwe-PCR. I-D-Taq DNA polymerase inokubekezelelana okuqinile kuma-PCR reaction inhibitors ahlukahlukene, futhi ingakhulisa kahle inani lokulandela ngomkhondo le-DNA ezinhlelweni ezahlukahlukene zokuphendula eziyinkimbinkimbi, futhi ijubane lokukhulisa lingafinyelela ku-2Kb / min, elifanele ikakhulukazi ukusabela okuqondile kwe-PCR Direct.

  • Zebra Fish Direct PCR Kit

    I-Zebra Fish Direct PCR Kit

    Leli thuluzi lisebenzisa uhlelo oluyingqayizivele lwe-lysis buffer ukukhipha ngokushesha i-genomic DNA kusuka kuma-zebrafish nakwezinye izicubu zezinhlanzi zasemanzini angenasawoti, amaphiko omsila noma amasampula amaqanda ezinhlanzi ekuphenduleni kwe-PCR, ngakho-ke kufaneleke kakhulu ukuhlolwa kwezakhi zofuzo ezinkulu. Inqubo yokukhipha i-genomic DNA kusuka kusigaxa se-lysis iphothulwe kungakapheli imizuzu eyi-10-30 ku-65 ° C. Azikho ezinye izinqubo ezifana namaprotheni nokususwa kwe-RNA okudingekayo, futhi umkhondo we-DNA okhishiwe ungasetshenziswa njengesifanekiso sokuphendula kwe-PCR.

    I-2 × PCR Easy TM Mix inokubekezelelana okuqinile kuma-PCR reaction inhibitors, futhi ingasebenzisa i-lysate yesampula ezohlolwa njengesifanekiso sokukhulisa okusebenzayo futhi okuqondile. Le reagent iqukethe i-ForegeneD-Taq DNAPolymerase, i-dNTPs, i-MgCl2, i-reaction buffer, i-PCR optimizer kanye ne-stabilizer. Isetshenziswe ngokubambisana ne-lysis buffer ingathola amasampula ngokushesha futhi kalula, futhi inezici zokuzwela okuphezulu, ukucaciswa okuqinile nokuqina okuhle.

    I-D-Taq DNA polymerase iyi-DNA polymerase eyenziwe ngokukhethekile yi-Foregene yokuphendula ngqo kwe-PCR. I-D-Taq DNA polymerase inokubekezelelana okuqinile kuma-PCR reaction inhibitors ahlukahlukene, futhi ingakhulisa kahle inani lokulandela ngomkhondo le-DNA ezinhlelweni ezahlukahlukene zokuphendula eziyinkimbinkimbi, futhi ijubane lokukhulisa lingafinyelela ku-2Kb / min, elifanele ikakhulukazi ukusabela okuqondile kwe-PCR Direct.

  • Zebra Fish Direct PCR Kit-UNG

    I-Zebra Fish Direct PCR Kit-UNG

    Leli thuluzi lisebenzisa uhlelo oluyingqayizivele lwe-lysis buffer ukukhipha ngokushesha i-genomic DNA kusuka kuma-zebrafish nakwezinye izicubu zezinhlanzi zasemanzini angenasawoti, amaphiko omsila noma amasampula amaqanda ezinhlanzi ekuphenduleni kwe-PCR, ngakho-ke kufaneleke kakhulu ukuhlolwa kwezakhi zofuzo ezinkulu. Inqubo yokukhipha i-genomic DNA kusuka kusigaxa se-lysis iphothulwe kungakapheli imizuzu eyi-10-30 ku-65 ° C. Azikho ezinye izinqubo ezifana namaprotheni nokususwa kwe-RNA okudingekayo, futhi umkhondo we-DNA okhishiwe ungasetshenziswa njengesifanekiso sokuphendula kwe-PCR.

    I-2 × PCR Easy TM Mix (UNG) isebenzisa i-DUTP esikhundleni se-dTTP ngesisekelo se-2 × PCR Easy TM Mix, futhi ingeza i-enzyme ye-UNG (i-Uracil-N-glycosylase) engalulaza isifanekiso esine-DUTP ngasikhathi sinye. Ngaphambi kokuphendula kwe-PCR, i-enzyme ye-UNG isetshenziselwa ukwehlisa umkhiqizo we-PCR oqukethe i-uracil. I-enzyme ye-UNG ngeke ibe nomthelela kuthempulethi engenayo i-uracil, ngaleyo ndlela iqinisekise ukucaciswa nokunemba kokukhulisa nokuvimbela ukuthi kungenzeka ukuhlolwa okukhulu kofuzo. Ukungcoliswa kwemikhiqizo ye-PCR kwenzeke.

    I-D-Taq DNA polymerase iyi-DNA polymerase eyenziwe ngokukhethekile yi-Foregene yokuphendula ngqo kwe-PCR. I-D-Taq DNA polymerase inokubekezelelana okuqinile kuma-PCR reaction inhibitors ahlukahlukene, futhi ingakhulisa kahle inani lokulandela ngomkhondo le-DNA ezinhlelweni ezahlukahlukene zokuphendula eziyinkimbinkimbi, futhi ijubane lokukhulisa lingafinyelela ku-2Kb / min, elifanele ikakhulukazi ukusabela okuqondile kwe-PCR Direct.