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I-Animal Tissue Direct PCR kit-UNG
Leli thuluzi lisebenzisa uhlelo oluyingqayizivele lwe-lysis buffer ukukhipha ngokushesha i-genomic DNA kusuka kumasampula wezicubu zezilwane ekuphenduleni kwe-PCR, ngakho-ke ilungele ikakhulukazi ukuhlolwa okukhulu kofuzo.
Inqubo yokukhipha i-genomic DNA kusuka kusigaxa se-lysis iphothulwe kungakapheli imizuzu eyi-10-30 ku-65°C. Azikho ezinye izinqubo ezifana namaprotheni nokususwa kwe-RNA okudingekayo, futhi umkhondo we-DNA okhishiwe ungasetshenziswa njengethempulethi yokuphendula kwe-PCR.
2×PCR EasyTM IMix (UNG) isebenzisa i-DUTP esikhundleni se-dTTP ngesisekelo se-2×PCR EasyTM Hlanganisa, bese ungeza i-enzyme ye-UNG (i-Uracil-N-glycosylase) engalulaza isifanekiso esine-DUTP ngasikhathi sinye. Ngaphambi kokuphendula kwe-PCR, i-enzyme ye-UNG isetshenziselwa ukwehlisa umkhiqizo we-PCR oqukethe i-uracil. I-enzyme ye-UNG ngeke ibe nomthelela kuthempulethi engenayo i-uracil, ngaleyo ndlela iqinisekise ukucaciswa nokunemba kokukhulisa nokuvimbela ukuthi kungenzeka ukungcoliswa kwemikhiqizo ye-PCR ngesikhathi sokuhlolwa okukhulu kofuzo.
I-D-Taq DNA polymerase iyi-DNA polymerase eyenziwe ngokukhethekile yi-Foregene yokuphendula ngqo kwe-PCR. I-D-Taq DNA polymerase inokubekezelelana okuqinile kuma-PCR reaction inhibitors ahlukahlukene, futhi ingakhulisa kahle inani lokulandela ngomkhondo le-DNA ezinhlelweni ezahlukahlukene zokuphendula eziyinkimbinkimbi, futhi ijubane lokukhulisa lingafinyelela ku-2Kb / min, elifanele ikakhulukazi ukusabela okuqondile kwe-PCR Direct.
