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Igundane Umsila SuperDirect PCR Kit
Leli thuluzi lisebenzisa uhlelo oluyingqayizivele lwe-lysis buffer ukukhipha ngokushesha i-genomic DNA kusuka kumasampula wegundane nezindlebe zegundane ekuphenduleni kwe-PCR, ngakho-ke ilungele ikakhulukazi ukuhlolwa okukhulu kofuzo.
Inqubo yokukhipha i-genomic DNA kusuka kusigaxa se-lysis isiqediwe kungakapheli imizuzu eyi-10-30 ekamelweni lokushisa (20-25 ° C). Akukho ukushisa okudingekayo, futhi ezinye izinqubo ezinjenge-protein ne-RNA ukususwa akudingeki. Inani elilandelwayo le-DNA lingasetshenziswa njengesifanekiso sokuphendula kwe-PCR.
I-2 × M-PCR Easy TM Mix inokubekezelelana okuqinile kuma-PCR reaction inhibitors, futhi ingasebenzisa i-lysate yesampula ezohlolwa njengesifanekiso sokukhulisa okusebenzayo futhi okuqondile. Le reagent iqukethe i-Foregene D-Taq DNAPolymerase, i-dNTPs, i-MgCl2, i-reaction buffer, i-PCR optimizer kanye ne-stabilizer.
I-D-Taq DNA polymerase iyi-DNA polymerase eyenziwe ngokukhethekile yi-Foregene yokuphendula ngqo kwe-PCR. I-D-Taq DNA polymerase inokubekezelelana okuqinile kuma-PCR reaction inhibitors ahlukahlukene, futhi ingakhulisa kahle inani lokulandela ngomkhondo le-DNA ezinhlelweni ezahlukahlukene zokuphendula eziyinkimbinkimbi, futhi ijubane lokukhulisa lingafinyelela ku-2Kb / min, elifanele ikakhulukazi ukusabela okuqondile kwe-PCR Direct.
