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Ikhithi ye-PCR Direct Tissue Direct
Leli thuluzi lisebenzisa uhlelo oluyingqayizivele lwe-lysis buffer ukukhipha ngokushesha i-genomic DNA kusuka kumasampula wezicubu zezilwane ekuphenduleni kwe-PCR, ngakho-ke ilungele ikakhulukazi ukuhlolwa okukhulu kofuzo.
Inqubo yokukhipha i-genomic DNA kusuka kusigaxa se-lysis iphothulwe kungakapheli imizuzu eyi-10-30 ku-65°C. Azikho ezinye izinqubo ezifana namaprotheni nokususwa kwe-RNA okudingekayo, futhi umkhondo we-DNA okhishiwe ungasetshenziswa njengesifanekiso sokuphendula kwe-PCR.
2× PCR EasyTM Imix inekubekezelela okuqinile kuma-PCR reaction inhibitors, futhi ingasebenzisa i-lysate yesampula ukuze ihlolwe njengesifanekiso sokukhulisa okusebenzayo futhi okuqondile. Le reagent iqukethe i-Foregene D-Taq DNAPolymerase, dNTPs, MgCl2, i-reaction buffer, i-PCR optimizer kanye ne-stabilizer.
I-D-Taq DNA polymerase iyi-DNA polymerase eyenziwe ngokukhethekile yi-Foregene yokuphendula ngqo kwe-PCR. I-D-Taq DNA polymerase inokubekezelelana okuqinile kuma-PCR reaction inhibitors ahlukahlukene, futhi ingakhulisa kahle inani lokulandela ngomkhondo le-DNA ezinhlelweni ezahlukahlukene zokuphendula eziyinkimbinkimbi, futhi ijubane lokukhulisa lingafinyelela ku-2Kb / min, elifanele ikakhulukazi ukusabela okuqondile kwe-PCR Direct.
