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Ikhithi ye-ForeSNP Genotyping
Ubuchwepheshe be-Competitive Allele Specific PCR (Competitive Allele Specific PCR) uhlobo olusha lwendlela yokuthayipha kwe-allele. Le ndlela ayidingi ukuhlanganisa ama-probe athile we-SNP ne-inDel ngayinye, kepha idinga kuphela ama-pair amabili we-probes yendawo yonke ehlukile ukuthola ukuthayipha okunembile kwamasampuli we-genomic DNA. Ngokuhlaziya amandla nobukhulu besiginali yokugcina ye-fluorescence, i-genotype inqunywa ngokuzenzakalela, futhi umphumela wokuhlangana uboniswa ngokubonakalayo. Le ndlela inesikhathi esifushane sokuthola, izindleko eziphansi ze-reagent, ukunemba okuphezulu kokuthola, futhi ingasetshenziselwa ukuzalaniswa komaka abasizwayo, ukubeka i-QTL, ukuhlonza umaki wofuzo nokunye ukuhlolwa kwamangqamuzana e-biology ngevolumu enkulu yesampula.
