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Iseli Direct RT qPCR Kit
Leli thuluzi lisebenzisa uhlelo oluyingqayizivele lwe-lysis buffer olungakhipha ngokushesha i-RNA kusuka kumasampuli amaseli akhulisiwe okuphendula kwe-RT-qPCR, ngaleyo ndlela kuqedwe inqubo yokuhlanzwa ye-RNA echitha isikhathi futhi ekhandlayo. Isifanekiso se-RNA singatholakala ngemizuzu engu-7 nje kuphela. Ama-reagents we-5 × Direct RT kanye ne-2 × Direct qPCR Mix-SYBR anikezwe yikhithi angathola ngokushesha nangempumelelo imiphumela yangempela ye-PCR yokulinganisa.
I-5 × Direct RT Mix ne-2 × Direct qPCR Mix-SYBR inokubekezelelana okuqinile kwe-inhibitor, futhi i-lysate yamasampuli ingasetshenziswa njengethempulethi ye-RT-qPCR ngqo. Le khithi iqukethe i-RNA ephezulu enobudlelwano obuphezulu be-Foregene reverse transcriptase, ne-Hot D-Taq DNA polymerase, i-dNTPs, i-MgCl2, i-reaction buffer, i-PCR optimizer kanye ne-stabilizer.
