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Ikhithi ye-PCR Direct Tissue Direct
Leli thuluzi lisebenzisa uhlelo oluyingqayizivele lwe-lysis buffer ukukhipha ngokushesha i-genomic DNA kusuka kumasampula wezicubu zezilwane ekuphenduleni kwe-PCR, ngakho-ke ilungele ikakhulukazi ukuhlolwa okukhulu kofuzo.
Inqubo yokukhipha i-genomic DNA kusuka kusigaxa se-lysis iphothulwe kungakapheli imizuzu eyi-10-30 ku-65°C. Azikho ezinye izinqubo ezifana namaprotheni nokususwa kwe-RNA okudingekayo, futhi umkhondo we-DNA okhishiwe ungasetshenziswa njengesifanekiso sokuphendula kwe-PCR.
2× PCR EasyTM Imix inekubekezelela okuqinile kuma-PCR reaction inhibitors, futhi ingasebenzisa i-lysate yesampula ukuze ihlolwe njengesifanekiso sokukhulisa okusebenzayo futhi okuqondile. Le reagent iqukethe i-Foregene D-Taq DNAPolymerase, dNTPs, MgCl2, i-reaction buffer, i-PCR optimizer kanye ne-stabilizer.
I-D-Taq DNA polymerase iyi-DNA polymerase eyenziwe ngokukhethekile yi-Foregene yokuphendula ngqo kwe-PCR. I-D-Taq DNA polymerase inokubekezelelana okuqinile kuma-PCR reaction inhibitors ahlukahlukene, futhi ingakhulisa kahle inani lokulandela ngomkhondo le-DNA ezinhlelweni ezahlukahlukene zokuphendula eziyinkimbinkimbi, futhi ijubane lokukhulisa lingafinyelela ku-2Kb / min, elifanele ikakhulukazi ukusabela okuqondile kwe-PCR Direct.
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Igundane Umsila SuperDirect PCR Kit
Leli thuluzi lisebenzisa uhlelo oluyingqayizivele lwe-lysis buffer ukukhipha ngokushesha i-genomic DNA kusuka kumasampula wegundane nezindlebe zegundane ekuphenduleni kwe-PCR, ngakho-ke ilungele ikakhulukazi ukuhlolwa okukhulu kofuzo.
Inqubo yokukhipha i-genomic DNA kusuka kusigaxa se-lysis isiqediwe kungakapheli imizuzu eyi-10-30 ekamelweni lokushisa (20-25 ° C). Akukho ukushisa okudingekayo, futhi ezinye izinqubo ezinjenge-protein ne-RNA ukususwa akudingeki. Inani elilandelwayo le-DNA lingasetshenziswa njengesifanekiso sokuphendula kwe-PCR.
I-2 × M-PCR Easy TM Mix inokubekezelelana okuqinile kuma-PCR reaction inhibitors, futhi ingasebenzisa i-lysate yesampula ezohlolwa njengesifanekiso sokukhulisa okusebenzayo futhi okuqondile. Le reagent iqukethe i-Foregene D-Taq DNAPolymerase, i-dNTPs, i-MgCl2, i-reaction buffer, i-PCR optimizer kanye ne-stabilizer.
I-D-Taq DNA polymerase iyi-DNA polymerase eyenziwe ngokukhethekile yi-Foregene yokuphendula ngqo kwe-PCR. I-D-Taq DNA polymerase inokubekezelelana okuqinile kuma-PCR reaction inhibitors ahlukahlukene, futhi ingakhulisa kahle inani lokulandela ngomkhondo le-DNA ezinhlelweni ezahlukahlukene zokuphendula eziyinkimbinkimbi, futhi ijubane lokukhulisa lingafinyelela ku-2Kb / min, elifanele ikakhulukazi ukusabela okuqondile kwe-PCR Direct.
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I-Animal Tissue Direct PCR kit-UNG
Leli thuluzi lisebenzisa uhlelo oluyingqayizivele lwe-lysis buffer ukukhipha ngokushesha i-genomic DNA kusuka kumasampula wezicubu zezilwane ekuphenduleni kwe-PCR, ngakho-ke ilungele ikakhulukazi ukuhlolwa okukhulu kofuzo.
Inqubo yokukhipha i-genomic DNA kusuka kusigaxa se-lysis iphothulwe kungakapheli imizuzu eyi-10-30 ku-65°C. Azikho ezinye izinqubo ezifana namaprotheni nokususwa kwe-RNA okudingekayo, futhi umkhondo we-DNA okhishiwe ungasetshenziswa njengethempulethi yokuphendula kwe-PCR.
2×PCR EasyTM IMix (UNG) isebenzisa i-DUTP esikhundleni se-dTTP ngesisekelo se-2×PCR EasyTM Hlanganisa, bese ungeza i-enzyme ye-UNG (i-Uracil-N-glycosylase) engalulaza isifanekiso esine-DUTP ngasikhathi sinye. Ngaphambi kokuphendula kwe-PCR, i-enzyme ye-UNG isetshenziselwa ukwehlisa umkhiqizo we-PCR oqukethe i-uracil. I-enzyme ye-UNG ngeke ibe nomthelela kuthempulethi engenayo i-uracil, ngaleyo ndlela iqinisekise ukucaciswa nokunemba kokukhulisa nokuvimbela ukuthi kungenzeka ukungcoliswa kwemikhiqizo ye-PCR ngesikhathi sokuhlolwa okukhulu kofuzo.
I-D-Taq DNA polymerase iyi-DNA polymerase eyenziwe ngokukhethekile yi-Foregene yokuphendula ngqo kwe-PCR. I-D-Taq DNA polymerase inokubekezelelana okuqinile kuma-PCR reaction inhibitors ahlukahlukene, futhi ingakhulisa kahle inani lokulandela ngomkhondo le-DNA ezinhlelweni ezahlukahlukene zokuphendula eziyinkimbinkimbi, futhi ijubane lokukhulisa lingafinyelela ku-2Kb / min, elifanele ikakhulukazi ukusabela okuqondile kwe-PCR Direct.
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Iseli Direct RT qPCR Kit
Leli thuluzi lisebenzisa uhlelo oluyingqayizivele lwe-lysis buffer olungakhipha ngokushesha i-RNA kusuka kumasampuli amaseli akhulisiwe okuphendula kwe-RT-qPCR, ngaleyo ndlela kuqedwe inqubo yokuhlanzwa ye-RNA echitha isikhathi futhi ekhandlayo. Isifanekiso se-RNA singatholakala ngemizuzu engu-7 nje kuphela. Ama-reagents we-5 × Direct RT kanye ne-2 × Direct qPCR Mix-SYBR anikezwe yikhithi angathola ngokushesha nangempumelelo imiphumela yangempela ye-PCR yokulinganisa.
I-5 × Direct RT Mix ne-2 × Direct qPCR Mix-SYBR inokubekezelelana okuqinile kwe-inhibitor, futhi i-lysate yamasampuli ingasetshenziswa njengethempulethi ye-RT-qPCR ngqo. Le khithi iqukethe i-RNA ephezulu enobudlelwano obuphezulu be-Foregene reverse transcriptase, ne-Hot D-Taq DNA polymerase, i-dNTPs, i-MgCl2, i-reaction buffer, i-PCR optimizer kanye ne-stabilizer.
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Umsila weMouse oqondile we-PCR Kit
Leli kit lisebenzisa uhlelo oluyingqayizivele lwe-lysis buffer ukukhipha ngokushesha i-genomic DNA kusuka emisileni yegundane, izindlebe, imisipha namanye amasampula wezicubu zokuphendula kwe-PCR, ngakho-ke ilungele ikakhulukazi ukuhlolwa okukhulu kofuzo.
Inqubo yokukhipha i-genomic DNA kusuka kusigaxa se-lysis iphothulwe kungakapheli imizuzu eyi-10-30 ku-65 ° C. Azikho ezinye izinqubo ezifana namaprotheni nokususwa kwe-RNA okudingekayo, futhi umkhondo we-DNA okhishiwe ungasetshenziswa njengesifanekiso sokuphendula kwe-PCR.
I-2 × M1-PCR Easy TM Mix inokubekezelelana okuqinile kuma-PCR reaction inhibitors, futhi ingasebenzisa i-lysate yesampula ezohlolwa njengesifanekiso sokukhulisa okusebenzayo futhi okuqondile. Le reagent iqukethe i-Foregene D-Taq DNAPolymerase, i-dNTPs, i-MgCl2, i-reaction buffer, i-PCR optimizer kanye ne-stabilizer.
I-D-Taq DNA polymerase iyi-DNA polymerase eyenziwe ngokukhethekile yi-Foregene yokuphendula ngqo kwe-PCR. I-D-Taq DNA polymerase inokubekezelelana okuqinile kuma-PCR reaction inhibitors ahlukahlukene, futhi ingakhulisa kahle inani lokulandela ngomkhondo le-DNA ezinhlelweni ezahlukahlukene zokuphendula eziyinkimbinkimbi, futhi ijubane lokukhulisa lingafinyelela ku-2Kb / min, elifanele ikakhulukazi ukusabela okuqondile kwe-PCR Direct.
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Umsila weMouse oqondile we-PCR Kit-UNG
Leli kit lisebenzisa uhlelo oluyingqayizivele lwe-lysis buffer ukukhipha ngokushesha i-genomic DNA kusuka emisileni yegundane, izindlebe, imisipha namanye amasampula wezicubu zokuphendula kwe-PCR, ngakho-ke ilungele ikakhulukazi ukuhlolwa okukhulu kofuzo.
Inqubo yokukhipha i-genomic DNA kusuka kusigaxa se-lysis iphothulwe kungakapheli imizuzu eyi-10-30 ku-65 ° C. Azikho ezinye izinqubo ezifana namaprotheni nokususwa kwe-RNA okudingekayo, futhi umkhondo we-DNA okhishiwe ungasetshenziswa njengesifanekiso sokuphendula kwe-PCR.
I-2 × M1-PCR Easy TM Mix (UNG) isebenzisa i-DUTP esikhundleni se-dTTP ngesisekelo se-2 × M1-PCR Easy TM Mix (UNG), futhi ingeza i-enzyme ye-UNG (i-Uracil-N-glycosylase) engehlisa isifanekiso esine-DUTP Ngisikhathi esifanayo. Ngaphambi kokuphendula kwe-PCR, i-enzyme ye-UNG isetshenziselwa ukwehlisa umkhiqizo we-PCR oqukethe i-uracil. I-enzyme ye-UNG ngeke ibe nomthelela kuthempulethi engenayo i-uracil, ngaleyo ndlela iqinisekise ukucaciswa nokunemba kokukhulisa nokuvimbela ukuthi kungenzeka ukuhlolwa okukhulu kofuzo. Ukungcoliswa kwemikhiqizo ye-PCR kwenzeke.
I-D-Taq DNA polymerase iyi-DNA polymerase eyenziwe ngokukhethekile yi-Foregene yokuphendula ngqo kwe-PCR. I-D-Taq DNA polymerase inokubekezelelana okuqinile kuma-PCR reaction inhibitors ahlukahlukene, futhi ingakhulisa kahle inani lokulandela ngomkhondo le-DNA ezinhlelweni ezahlukahlukene zokuphendula eziyinkimbinkimbi, futhi ijubane lokukhulisa lingafinyelela ku-2Kb / min, elifanele ikakhulukazi ukusabela okuqondile kwe-PCR Direct.
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I-Zebra Fish Direct PCR Kit
Leli thuluzi lisebenzisa uhlelo oluyingqayizivele lwe-lysis buffer ukukhipha ngokushesha i-genomic DNA kusuka kuma-zebrafish nakwezinye izicubu zezinhlanzi zasemanzini angenasawoti, amaphiko omsila noma amasampula amaqanda ezinhlanzi ekuphenduleni kwe-PCR, ngakho-ke kufaneleke kakhulu ukuhlolwa kwezakhi zofuzo ezinkulu. Inqubo yokukhipha i-genomic DNA kusuka kusigaxa se-lysis iphothulwe kungakapheli imizuzu eyi-10-30 ku-65 ° C. Azikho ezinye izinqubo ezifana namaprotheni nokususwa kwe-RNA okudingekayo, futhi umkhondo we-DNA okhishiwe ungasetshenziswa njengesifanekiso sokuphendula kwe-PCR.
I-2 × PCR Easy TM Mix inokubekezelelana okuqinile kuma-PCR reaction inhibitors, futhi ingasebenzisa i-lysate yesampula ezohlolwa njengesifanekiso sokukhulisa okusebenzayo futhi okuqondile. Le reagent iqukethe i-ForegeneD-Taq DNAPolymerase, i-dNTPs, i-MgCl2, i-reaction buffer, i-PCR optimizer kanye ne-stabilizer. Isetshenziswe ngokubambisana ne-lysis buffer ingathola amasampula ngokushesha futhi kalula, futhi inezici zokuzwela okuphezulu, ukucaciswa okuqinile nokuqina okuhle.
I-D-Taq DNA polymerase iyi-DNA polymerase eyenziwe ngokukhethekile yi-Foregene yokuphendula ngqo kwe-PCR. I-D-Taq DNA polymerase inokubekezelelana okuqinile kuma-PCR reaction inhibitors ahlukahlukene, futhi ingakhulisa kahle inani lokulandela ngomkhondo le-DNA ezinhlelweni ezahlukahlukene zokuphendula eziyinkimbinkimbi, futhi ijubane lokukhulisa lingafinyelela ku-2Kb / min, elifanele ikakhulukazi ukusabela okuqondile kwe-PCR Direct.
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I-Zebra Fish Direct PCR Kit-UNG
Leli thuluzi lisebenzisa uhlelo oluyingqayizivele lwe-lysis buffer ukukhipha ngokushesha i-genomic DNA kusuka kuma-zebrafish nakwezinye izicubu zezinhlanzi zasemanzini angenasawoti, amaphiko omsila noma amasampula amaqanda ezinhlanzi ekuphenduleni kwe-PCR, ngakho-ke kufaneleke kakhulu ukuhlolwa kwezakhi zofuzo ezinkulu. Inqubo yokukhipha i-genomic DNA kusuka kusigaxa se-lysis iphothulwe kungakapheli imizuzu eyi-10-30 ku-65 ° C. Azikho ezinye izinqubo ezifana namaprotheni nokususwa kwe-RNA okudingekayo, futhi umkhondo we-DNA okhishiwe ungasetshenziswa njengesifanekiso sokuphendula kwe-PCR.
I-2 × PCR Easy TM Mix (UNG) isebenzisa i-DUTP esikhundleni se-dTTP ngesisekelo se-2 × PCR Easy TM Mix, futhi ingeza i-enzyme ye-UNG (i-Uracil-N-glycosylase) engalulaza isifanekiso esine-DUTP ngasikhathi sinye. Ngaphambi kokuphendula kwe-PCR, i-enzyme ye-UNG isetshenziselwa ukwehlisa umkhiqizo we-PCR oqukethe i-uracil. I-enzyme ye-UNG ngeke ibe nomthelela kuthempulethi engenayo i-uracil, ngaleyo ndlela iqinisekise ukucaciswa nokunemba kokukhulisa nokuvimbela ukuthi kungenzeka ukuhlolwa okukhulu kofuzo. Ukungcoliswa kwemikhiqizo ye-PCR kwenzeke.
I-D-Taq DNA polymerase iyi-DNA polymerase eyenziwe ngokukhethekile yi-Foregene yokuphendula ngqo kwe-PCR. I-D-Taq DNA polymerase inokubekezelelana okuqinile kuma-PCR reaction inhibitors ahlukahlukene, futhi ingakhulisa kahle inani lokulandela ngomkhondo le-DNA ezinhlelweni ezahlukahlukene zokuphendula eziyinkimbinkimbi, futhi ijubane lokukhulisa lingafinyelela ku-2Kb / min, elifanele ikakhulukazi ukusabela okuqondile kwe-PCR Direct.
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Tshala iqabunga lekhithi le-PCR Plus
Lo mkhiqizo usebenzisa uhlelo oluyingqayizivele lwe-lysis buffer ukuze uvuse amaqabunga ezitshalo. I-lysate ingasetshenziswa njengesifanekiso ngaphandle kokuhlanzwa. Ngemuva kokungeza ama-primers, i-PCR Mix yale kit ingasetshenziselwa ukukhulisa.
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Tshala Imbewu Iqonde ngqo ku-PCR Kit I / II
Le khithi isebenzisa uhlelo oluyingqayizivele lwe-lysis buffer ukuze i-lyse imbewu yezitshalo. I-lysate ingasetshenziswa njengesifanekiso ngaphandle kokuhlanzwa. Ngemuva kokungeza ama-primers, i-PCR Mix yale kit ingasetshenziselwa ukukhulisa.
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Tshala iqabunga le-PCR eqondile kanye ne-kit-UNG
Ngokuya ngekhithi yePlant Leaf Direct PCR Plus, i-DUTP isetshenziswa esikhundleni se-dTTP, futhi kufakwa i-enzyme ye-UNG engehlisa isifanekiso esine-DUTP. Ngale ndlela, ukucaciswa nokunemba kokukhulisa amandla kuyaqinisekiswa, futhi inkinga yokungcola komkhiqizo we-PCR okungenzeka ngesikhathi sokuhlolwa kwezakhi zofuzo ezinkulu kuvinjelwe.
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Tshala iqabunga i-PCR kit-UNG ngqo
Ngokuya ngekhithi yePlant Leaf Direct PCR, i-DUTP isetshenziswa esikhundleni se-dTTP, futhi kufakwa i-enzyme ye-UNG engehlisa isifanekiso esine-DUTP. Ngale ndlela, ukucaciswa nokunemba kokukhulisa amandla kuyaqinisekiswa, futhi inkinga yokungcola komkhiqizo we-PCR okungenzeka ngesikhathi sokuhlolwa kwezakhi zofuzo ezinkulu kuvinjelwe.
