Ngokuvamile sikholelwa ukuthi umlingiswa womuntu unquma ikhwalithi ephezulu yemikhiqizo, imininingwane inquma izinga eliphezulu lemikhiqizo, kanye nomoya weqembu OBUYIQINISO, OSEBENZA KANYE OKUSHA OKUSHA mayelana Nokulethwa OkushaI-China Cell Total Rna Isolation KitI-Rnase-Free CE, intengo yokuthengisa enolaka enekhwalithi ephezulu kanye nezinsizakalo ezanelisayo zisenza sizuze abathengi abaningi kakhulu.sifisa ukusebenzisana nawe futhi sibheke ukuthuthukiswa okufanayo.
Ngokuvamile sikholelwa ukuthi umlingiswa womuntu unquma izinga eliphezulu lemikhiqizo, imininingwane inquma izinga eliphezulu lemikhiqizo, kanye nomoya weqembu OWAQINISEKILE, OSEBENZA KANYE NOBUSHAIseli, I-China Cell Total Rna Isolation Kit, Inkampani yethu isivele iliphasile izinga le-ISO futhi siwahlonipha ngokugcwele amalungelo obunikazi amakhasimende ethu namalungelo okushicilela.Uma ikhasimende lihlinzeka ngemiklamo yalo, Sizoqinisekisa ukuthi cishe kuzoba yilona kuphela elingaba nalokho kuthengiswa.Siyethemba ukuthi ngemikhiqizo yethu emihle ingalethela amakhasimende ethu inhlanhla enkulu.

Incazelo

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Le khithi isebenzisa ikholomu yokuphotha kanye nefomula eyakhiwe yi-Foregene, engakhipha ngempumelelo i-RNA ephezulu nekhwalithi ephezulu kumaseli akhuliswe ngamapuleti emithombo engu-96, 24, 12, kanye ne-6.

Ikhithi inikeza Ikholomu Yokuhlanza I-DNA esebenza kahle, engahlukanisa kalula i-supernatant ne-cell lysate, ibophe futhi isuse i-genomic DNA.Ukusebenza kulula futhi konga isikhathi.

Ikholomu ye-RNA kuphela ingahlanganisa ngempumelelo i-RNA ngefomula ehlukile.Inani elikhulu lamasampula lingacutshungulwa kanyekanye.

Izingxenye zekhithi

*Sicela ugqoke amagilavu ​​futhi uthathe izinyathelo zokuvikela ngesikhathi sokusebenza njengoba i-Buffer cRL1 ne-Buffer RW1 iqukethe usawoti we-chaotropic ocasulayo.

Izici&izinzuzo

■ Yonke inqubo iqhutshwa ekamelweni lokushisa (15-25 ℃), ngaphandle kokugeza kweqhwa kanye nokushisa okuphansi kwe-centrifugation.
■ Ikhithi yonke ayinayo i-RNase-Free, asikho isidingo sokukhathazeka ngokuwohloka kwe-RNA.
■ Ikholomu Yokuhlanza I-DNA ibopha ngokukhethekile i-DNA, ukuze ikhithi ikwazi ukususa ukungcola kwe-DNA ye-genomic ngaphandle kokwengeza i-DNase eyengeziwe.
■ Isivuno esikhulu se-RNA: Ikholomu ye-RNA kuphela kanye nefomula eyingqayizivele ingahlanza i-RNA ngokuphumelelayo.
■ Isivinini esisheshayo: kulula ukusebenza futhi singaqedwa ngemizuzu eyi-11.
■ Ukuphepha: Asikho i-reagent ephilayo edingekayo.
■ Ikhwalithi ephezulu: I-RNA ehlanziwe imsulwa kakhulu, ayinamaprotheni nokunye ukungcola, futhi ingahlangabezana nokuhlola okuhlukahlukene okulandelayo.

Isicelo sekhithi

Ilungele ukukhishwa nokuhlanzwa kwe-RNA ephelele kumaseli akhulisiwe kumapuleti angama-96, 24, 12, kanye namapuleti angu-6.

Ukugeleza komsebenzi

Umdwebo

Umdwebo webhethri yejeli ye-agarose yeIseliIngqikithi ye-RNA Isolation Kit iphathe izinombolo ezingenhla ezihlukene zamaseli, ukukhishwa kwevolumu engu-20μl, thatha u-2μl isamba esihlanziwe se-RNA 1%.

Isitoreji nempilo yeshelufu

Ikhithi ingagcinwa izinyanga eziyi-12 ekamelweni lokushisa (15-25 ℃) noma 2-8 ℃ isikhathi eside (izinyanga ezingama-24).

I-Buffer cRL1 ingagcinwa ku-4 ℃ inyanga engu-1 ngemva kokwengeza i-2-hydroxy-1-ethanethiol(ongakukhetha).Ngokujwayelekile sikholelwa ukuthi umlingiswa womuntu nguye onquma ikhwalithi ephezulu yemikhiqizo, imininingwane inquma izinga eliphezulu lemikhiqizo, kanye nomoya weqembu OYIMPELA, OKUSEBENZA KANYE OKUSHA OKUSHA kokulethwa kwe-China.IseliIngqikithi ye-Rna Isolation Kit Rnase-Free ISO FDA CE, intengo yokuthengisa enolaka enekhwalithi ephezulu kanye nezinsizakalo ezanelisayo zisenza sizuze abathengi abaningi kakhulu.sifisa ukusebenzisana nawe futhi sibheke ukuthuthukiswa okufanayo.
Ukulethwa Okusha kwe-China Cell Total Rna Isolation Kit, Cell, Inkampani yethu isivele iphumelele izinga le-ISO futhi siwahlonipha ngokugcwele amalungelo obunikazi amakhasimende ethu namalungelo okushicilela.Uma ikhasimende lihlinzeka ngemiklamo yalo, Sizoqinisekisa ukuthi cishe kuzoba yilona kuphela elingaba nalokho kuthengiswa.Siyethemba ukuthi ngemikhiqizo yethu emihle ingalethela amakhasimende ethu inhlanhla enkulu.