Sinalesi siqubulo engqondweni, sesiphenduke omunye wabakhiqizi okungenzeka ukuthi baqambe kakhulu, ababiza kakhulu, nabaqhudelana nentengo Yekhwalithi Ephezulu.I-China Nucleic Acid Extraction Reagent, I-Rna Extraction Test Kits Nucleic Acid Extraction Reagent, Siyakwamukela ukuthi nakanjani ube yingxenye yethu ndawonye ukuze wenze inhlangano yakho ibe lula.Besijwayele ukuba ngabalingani bakho abakhulu uma ufuna ukuba nebhizinisi lakho elincane.
Sinalesi siqubulo engqondweni, sesiphenduke omunye wabakhiqizi okungenzeka ukuthi baqambe kakhulu, ababiza kakhulu, nabancintisana namanani entengo.I-China Nucleic Acid Extraction Reagent, Indlela ye-Magnetic Bead, Sizokwenza konke okusemandleni ukubambisana futhi saneliseke ngawe sincike kwikhwalithi ephezulu kanye nenani lokuncintisana futhi okungcono kakhulu ngemva kwesevisi, ngobuqotho sibheke phambili ukubambisana nawe futhi senze impumelelo esikhathini esizayo!

Incazelo

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Le khithi isebenzisa ikholomu yokuphotha kanye nefomula eyakhiwe yi-Foregene, engakhipha ngempumelelo i-RNA ephezulu nekhwalithi ephezulu kumaseli akhuliswe ngamapuleti emithombo engu-96, 24, 12, kanye ne-6.

Ikhithi inikeza Ikholomu Yokuhlanza I-DNA esebenza kahle, engahlukanisa kalula i-supernatant ne-cell lysate, ibophe futhi isuse i-genomic DNA.Ukusebenza kulula futhi konga isikhathi.

Ikholomu ye-RNA kuphela ingahlanganisa ngempumelelo i-RNA ngefomula ehlukile.Inani elikhulu lamasampula lingacutshungulwa kanyekanye.

Izingxenye zekhithi

*Sicela ugqoke amagilavu ​​futhi uthathe izinyathelo zokuvikela ngesikhathi sokusebenza njengoba i-Buffer cRL1 ne-Buffer RW1 iqukethe usawoti we-chaotropic ocasulayo.

Izici&izinzuzo

■ Yonke inqubo iqhutshwa ekamelweni lokushisa (15-25 ℃), ngaphandle kokugeza kweqhwa kanye nokushisa okuphansi kwe-centrifugation.
■ Ikhithi yonke ayinayo i-RNase-Free, asikho isidingo sokukhathazeka ngokuwohloka kwe-RNA.
■ Ikholomu Yokuhlanza I-DNA ibopha ngokukhethekile i-DNA, ukuze ikhithi ikwazi ukususa ukungcola kwe-DNA ye-genomic ngaphandle kokwengeza i-DNase eyengeziwe.
■ Isivuno esikhulu se-RNA: Ikholomu ye-RNA kuphela kanye nefomula eyingqayizivele ingahlanza i-RNA ngokuphumelelayo.
■ Isivinini esisheshayo: kulula ukusebenza futhi singaqedwa ngemizuzu eyi-11.
■ Ukuphepha: Asikho i-reagent ephilayo edingekayo.
■ Ikhwalithi ephezulu: I-RNA ehlanziwe imsulwa kakhulu, ayinamaprotheni nokunye ukungcola, futhi ingahlangabezana nokuhlola okuhlukahlukene okulandelayo.

Isicelo sekhithi

Ilungele ukukhishwa nokuhlanzwa kwe-RNA ephelele kumaseli akhulisiwe kumapuleti angama-96, 24, 12, kanye namapuleti angu-6.

Ukugeleza komsebenzi

Umdwebo

Umdwebo webhethri yejeli ye-agarose ye-Cell Total RNA Isolation Kit iphathe izinombolo ezingenhla ezihlukene zamaseli, ukukhishwa kwevolumu engu-20μl, thatha u-2μl isamba esihlanziwe se-RNA 1%.

Isitoreji nempilo yeshelufu

Ikhithi ingagcinwa izinyanga eziyi-12 ekamelweni lokushisa (15-25 ℃) noma 2-8 ℃ isikhathi eside (izinyanga ezingama-24).

I-Buffer cRL1 ingagcinwa ku-4 ℃ inyanga engu-1 ngemva kokwengeza i-2-hydroxy-1-ethanethiol(ongakukhetha).Ngalesi siqubulo engqondweni, sesiphenduke omunye wabakhiqizi okungenzeka ukuthi baqambe kakhulu ubuchwepheshe, ababiza izindleko, nabaqhudelana nentengo Yekhwalithi Ephezulu.I-China Nucleic Acid Extraction Reagent, I-Rna Extraction Test Kits Nucleic Acid Extraction Reagent, Siyakwamukela ukuthi nakanjani ube yingxenye yethu ndawonye ukuze wenze inhlangano yakho ibe lula.Besijwayele ukuba ngabalingani bakho abakhulu uma ufuna ukuba nebhizinisi lakho elincane.
Ikhwalithi ephezulu yaseChina Nucleic Acid Extraction Reagent,Indlela ye-Magnetic Bead, Sizokwenza konke okusemandleni ukubambisana futhi saneliseke ngawe sincike kwikhwalithi ephezulu kanye nenani lokuncintisana futhi okungcono kakhulu ngemva kwesevisi, ngobuqotho sibheke phambili ukubambisana nawe futhi senze impumelelo esikhathini esizayo!