Futhi sisebenza ngokukhethekile ekuqiniseni ukuphathwa kwezinto kanye nendlela ye-QC ukuze siqinisekise ukuthi singagcina inzuzo enkulu ngenkathi sisebhizinisini eliqhudelana kakhulu le-Factory For China Techstar Magnetic Bead Method Swab Sample Nucleic Acid Extraction Kit Rna Isolation Reagent DNA Purification Kit, Ukubona kukholelwa!Siwamukela ngobuqotho amathuba amasha aphesheya okusungula ukusebenzisana kwenkampani futhi silindele ukuhlanganisa ukusebenzelana nawo wonke amakhasimende asenesikhathi eside asungulwa.
Sisebenza ngokukhethekile ekuqiniseni ukuphathwa kwezinto kanye nendlela ye-QC ukuze siqinisekise ukuthi singakwazi ukulondoloza inzuzo enkulu ngenkathi sisebhizinisini eliqhudelana kakhulu.I-China Nucleic Acid Reagent, Ikhithi yokukhipha i-DNA/Rna, Inkampani yethu ingumnikezeli wamazwe ngamazwe ngalolu hlobo lokuthengiswayo.Sinikeza ukukhetha okumangalisayo kwezixazululo zekhwalithi ephezulu.Umgomo wethu uwukujabulisa wena ngeqoqo lethu elihlukile lezinto ezinengqondo ngenkathi sinikeza inani kanye nesevisi enhle kakhulu.Umgomo wethu ulula: Ukunikeza imikhiqizo nesevisi engcono kakhulu kumakhasimende ethu ngamanani aphansi kakhulu ngangokunokwenzeka.

Imininingwane

50 Preps, yokuhlanzwa kwe-DNA ye-genomic egazini lonke kusetshenziswa<1mligazi

I-Buffer DC: Ihambisa amaseli abomvu egazi egazini.

I-Buffer BL1: Nikeza ngendawo yokugaya i-enzymatic eyisampula.

I-Foregene Protease Plus: Gaya amasampula egazi nge-enzyme endaweni ye-Buffer BL1.

I-Buffer BL2: Yenza i-Foregene Protease Plus isebenze futhi inikeze indawo yokulayisha i-DNA.

I-Buffer PW: Susa ukungcola okufana namaprotheni ne-RNA ku-DNA.

I-Buffer WB1: Susa ama-ion kasawoti asele ku-DNA.

I-Buffer EB: I-Elute i-DNA kulwelwesi lwekholomu yokuhlanza.

Ikholomu Ye-DNA Kuphela: Ikhangisa i-DNA ye-genomic ku-lysate.

Izingxenye zekhithi

Izici&izinzuzo

-Akukho ukungcola kwe-RNase: Ikholomu Ye-DNA Kuphela ehlinzekwa ikhithi yenza kube nokwenzeka ukususa i-RNA ku-DNA ye-genomic ngaphandle kokwengeza i-RNase phakathi nokuhlolwa, ukugwema ilabhorethri ukuthi ingangcoliswa i-RNase yangaphandle.

-Isivinini esisheshayo: I-Foregene Protease Plus inomsebenzi ophezulu kunama-protease afanayo, futhi igaya amasampula ezicubu ngokushesha;ukusebenza kulula, futhi ukusebenza kwe-genomic DNA extraction kungaqedwa phakathi nemizuzu engama-40.

-Okulula: I-centrifugation yenziwa ekamelweni lokushisa, i-4°C centrifugation noma i-ethanol precipitation ye-DNA ayidingeki.

-Ukuphepha: Akukho kukhishwa kwe-organic reagent esetshenziswayo.

-Ikhwalithi ephezulu: Izingcezu ze-genomic DNA ezikhishiwe zinkulu, azikho i-RNA, azinayo i-RNase, nokuqukethwe kwe-ion okuphansi kakhulu, okungahlangabezana nezidingo zokuhlolwa okuhlukahlukene.

Isicelo sekhithi

Ilungele ukukhishwa kanye nokuhlanzwa kwe-genomic DNA egazini elisha noma eliqandisiwe elilwa nokuqunjelwa.

Ukuhamba komsebenzi

Isitoreji kanye Nempilo yeshelufu

Le kit ingagcinwa izinyanga ezingu-12 ngaphansi kwezimo ezomile ekamelweni lokushisa (15-25 ° C);uma idinga ukugcinwa isikhathi eside, ingagcinwa ku-2-8°C.

Qaphela: Uma igcinwe ezingeni lokushisa eliphansi, isixazululo sithambekele ekubeni nemvula.Ngaphambi kokusetshenziswa, qiniseka ukuthi ubeka ikhambi kukhithi ekamelweni lokushisa isikhathi esithile.Uma kunesidingo, yishise ngaphambili emanzini okugeza angu-37 ° C imizuzu engu-10 ukuze uhlakaze i-precipitate, futhi uyixube ngaphambi kokusetshenziswa.

Isixazululo se-Foregene Protease Plus sinefomula eyingqayizivele, esebenzayo uma igcinwe ekamelweni lokushisa isikhathi eside (izinyanga ezi-3);izosebenza kakhulu futhi izinzile uma igcinwe ku-4 ° C, ngakho-ke kuyatuswa ukuyigcina ku-4 ° C, khumbula ukuthi ungayigcini ku -20 ° C. Sisebenza ngokukhethekile ekuqiniseni ukuphathwa kwezinto kanye nendlela ye-QC ukuze siqinisekise ukuthi singagcina inzuzo enkulu ngenkathi sisebhizinisini eliqhudelana kakhulu le-Factory For China Techstar Magnetic Bead Acculation Kixid Explosion Kixidna I-SwawD , Ukubona kuyakholwa!Siwamukela ngobuqotho amathuba amasha aphesheya okusungula ukusebenzisana kwenkampani futhi silindele ukuhlanganisa ukusebenzelana nawo wonke amakhasimende asenesikhathi eside asungulwa.
Factory ForI-China Nucleic Acid Reagent, Ikhithi yokukhipha i-DNA/Rna, Inkampani yethu ingumnikezeli wamazwe ngamazwe ngalolu hlobo lokuthengiswayo.Sinikeza ukukhetha okumangalisayo kwezixazululo zekhwalithi ephezulu.Umgomo wethu uwukujabulisa wena ngeqoqo lethu elihlukile lezinto ezinengqondo ngenkathi sinikeza inani kanye nesevisi enhle kakhulu.Umgomo wethu ulula: Ukunikeza imikhiqizo nesevisi engcono kakhulu kumakhasimende ethu ngamanani aphansi kakhulu ngangokunokwenzeka.