Sithola injabulo ekudumeni okuhle okumangalisayo phakathi kwamakhasimende ethu ngokuthengiswa kwethu okuhle kwekhwalithi ephezulu, okunolaka kanye nokwesekwa okuphumelelayo Kwesithunzi Somsebenzisi Esihle se-China Nucleic Acid Extraction Kit Yokuhlola I-DNA Yegazi I-Nucleic Acid Purification Reagent, Amathemba kuqala!Noma ngabe yini oyidingayo, kufanele senze konke esingakwenza ukuze sikusize.Samukela ngemfudumalo amaklayenti avela kuwo wonke umhlaba ukuthi abambisane nathi ukuze sithuthukisane.
Sithola injabulo ngokuduma okuhle ngendlela emangalisayo phakathi kwamakhasimende ethu ngokuthengiswa kwethu okuhle kwekhwalithi ephezulu, izinga elinamandla kanye nokusekelwa okusebenzayoI-China Nucleic Acid Extraction Reagent, Indlela ye-Magnetic Bead, Sinonjiniyela abaphezulu kulezi zimboni kanye nethimba elisebenza kahle ocwaningweni.Ngaphezu kwalokho, manje sinezinqolobane zethu zomlando nezimakethe e-China ngezindleko eziphansi.Ngakho-ke, singakwazi ukuhlangabezana nemibuzo ehlukene evela kumakhasimende ahlukene.Khumbula ukuthola iwebhusayithi yethu ukuze uhlole imininingwane eyengeziwe ezintweni zethu.

Kits Izincazelo

50 Preps, 200 Preps

Le kit isebenzisa ikholomu ye-spin kanye nefomula eyakhiwe inkampani yethu, engakwazi ukukhipha i-RNA ephezulu kanye nekhwalithi ephezulu engqikithini yezilwane ezihlukahlukene ngokusebenza kahle okuphezulu.Ihlinzeka ngekholomu ye-DNA-Cleaning ephumelelayo, engakwazi ukuhlukanisa kalula futhi ikhangise i-DNA ye-genomic ku-supernatant kanye ne-tissue lysate, elula futhi elondoloza isikhathi;Ikholomu ye-RNA kuphela ingahlanganisa ngempumelelo i-RNA futhi ingacutshungulwa kanyekanye ngefomula eyingqayizivele Amasampuli amaningi.

Lonke uhlelo luyi-RNase-Free, ukuze i-RNA ekhishiwe ingonakalisiwe;I-Buffer RW1, i-Buffer RW2 isistimu yokugeza ibhafa, ukuze i-RNA etholiwe ingabi nawo amaprotheni, i-DNA, i-ion, kanye nokungcoliswa kwenhlanganisela yezinto eziphilayo.

Izingxenye zekhithi:

Izici&izinzuzo

■ Asikho isidingo sokukhathazeka ngokuwohloka kwe-RNA;lonke uhlelo luyi-RNase-Free
■ Khipha ngempumelelo i-DNA usebenzisa i-DNA-Cleaning Column
■ Khipha i-DNA ngaphandle kokwengeza i-DNase
■ Kulula-yonke imisebenzi iqedwa ekamelweni lokushisa
■ Ukusebenza okusheshayo kungaqedwa ngemizuzu engama-30
■ I-Safe-akukho reagent ephilayo edingekayo
■ Ukuhlanzeka okuphezulu -OD260/280≈1.8-2.1

Isicelo sekhithi

Ilungele ukukhishwa nokuhlanzwa kwe-RNA ephelele ezinhlobonhlobo zezicubu zezilwane ezintsha noma eziqandisiwe noma amaseli akhulisiwe.

Imingcele yomkhiqizo

■ Izinhlelo zokusebenza eziwela phansi: ukuhlanganiswa kwe-cDNA ye-first-strand, i-RT-PCR, i-molecular cloning, i-Northern Blot, njll.
■ Amasampula: izicubu zezilwane, amaseli akhulisiwe
■ Umthamo: Izicubu 10-20mg, Amaseli(2-5)×10⁶
■ Umthamo omkhulu wokubopha we-DNA wekholomu yokuhlanza: 80 μg
■ Ivolumu ye-Elution: 50-200 μl

Ukugeleza komsebenzi

Umdwebo

I-Animal Total RNA Isolation Kit iphathwe ama-20mg
Amasampula egundane amasha, thatha i-RNA ephelele engu-5% 1% ye-agar

I-Glycogel electrophoresis
1: Ubende 2: Izinso
3: Isibindi 4: Inhliziyo

Isitoreji nempilo yeshelufu

Ikhithi ingagcinwa izinyanga ezingama-24 ekamelweni lokushisa (15-25 ℃) noma 2–8 ℃ isikhathi eside.I-Buffer RL1 ingagcinwa ku-4 ℃ inyanga engu-1 ngemva kokwengeza i-β- mercaptoethanol (uma uthanda).

Sithola injabulo ekudumeni okuhle okumangalisayo phakathi kwamakhasimende ethu ngokuthengiswa kwethu okuhle kwekhwalithi ephezulu, okunolaka kanye nokwesekwa okuphumelelayo Kwesithunzi Somsebenzisi Esihle se-China Nucleic Acid Extraction Kit Yokuhlola I-DNA Yegazi I-Nucleic Acid Purification Reagent, Amathemba kuqala!Noma ngabe yini oyidingayo, kufanele senze konke esingakwenza ukuze sikusize.Samukela ngemfudumalo amaklayenti avela kuwo wonke umhlaba ukuthi abambisane nathi ukuze sithuthukisane.
Idumela elihle lomsebenzisiI-China Nucleic Acid Extraction Reagent, Indlela ye-Magnetic Bead, Sinonjiniyela abaphezulu kulezi zimboni kanye nethimba elisebenza kahle ocwaningweni.Ngaphezu kwalokho, manje sinezinqolobane zethu zomlando nezimakethe e-China ngezindleko eziphansi.Ngakho-ke, singakwazi ukuhlangabezana nemibuzo ehlukene evela kumakhasimende ahlukene.Khumbula ukuthola iwebhusayithi yethu ukuze uhlole imininingwane eyengeziwe ezintweni zethu.