Sikholelwa kulokhu: Ukuqamba okusha kungumphefumulo nomoya wethu.Izinga eliphezulu liyimpilo yethu.Isidingo somthengi nguNkulunkulu wethu ngentengo ecashuniwe ye-Total Rna Reverse Transcription Kit Mirna First Strand Cdna Synthesis Kit Tailing, Siyakwamukela ukusivakashela nganoma yisiphi isikhathi ukuze usungulwe umshado wenhlangano.
Sikholelwa kulokhu: Ukuqamba okusha kungumphefumulo nomoya wethu.Izinga eliphezulu liyimpilo yethu.Isidingo somthengi singuNkulunkulu wethuI-China Total Rna Reverse Transcription kanye ne-Cdna Synthesis Tailing Kit, Kukhona imishini ethuthukisiwe yokukhiqiza & yokucubungula kanye nabasebenzi abanamakhono ukuze baqinisekise izinto ngekhwalithi ephezulu.Sithole isevisi enhle kakhulu yangaphambi kokuthengisa, yokuthengisa, yangemuva kokuthengisa ukuze siqinisekise amakhasimende angaqiniseka ukwenza ama-oda.Kuze kube manje izixazululo zethu manje zihamba ngokushesha futhi zithandwa kakhulu eNingizimu Melika, eMpumalanga Asia, eMpumalanga Ephakathi, e-Afrika, njll.

Imininingwane

5ml, 10ml, 40ml

I-PCR Hero (Nge-Dye) Ikhithi yokukhipha ihlanganisa isizukulwane esisha esiyingqayizivele sika-Foregene se-Taq—Foregene Taq Hero, ama-dNTP athuthukisiwe, i-MgCl2, i-react buffer, i-PCR reaction enhancer, i-optimizer nesiqinisi.Uhlelo lwe-2× PCR Hero TM Mix (udayi) lunokubekezelela okuphezulu kwe-PCR inhibitors kunohlelo olujwayelekile lwe-PCR Mix, futhi lungabhekana kalula nokukhulisa i-PCR yezifanekiso eziyinkimbinkimbi ezihlukahlukene.Isistimu yokusabela eyingqayizivele kanye nokusebenza kahle okuphezulu kwe-Taq Hero kwenza ukusabela kwe-PCR kube nokusebenza kahle kokukhulisa ukuphakama, ukucacisa nokuzwela.

Izingxenye zekhithi

Izici&izinzuzo

■ Ukwethembeka okuphezulu: izikhathi eziyisi-6 kune-enzyme ye-Taq evamile;

■ Isivinini sokukhulisa esisheshayo : ijubane lokukhulisa ngu-5-10sec/kb, eliphindwe izikhathi ezingu-6-12 kune-enzyme ye-Taq evamile;

■ Ukuguquguquka okwengeziwe kwesifanekiso: Ingakwazi ukukhulisa izifanekiso ze-DNA eziyinkimbinkimbi ngokuphezuluOkuqukethwe kwe-GC;

■ Ukusebenza okuphakeme kokukhulisa amandla: imijikelezo yokukhulisa ephansi kune-enzyme ye-Taq evamile;

∎ Ukubekezelelana kwemvelo kunamandla: ibekwe ku-37°C isonto lonke, igcina umsebenzi ongaphezu kwama-90%;

■ Inomsebenzi ongu-5′ →3′ we-DNA polymerase kanye no-5′→3′ umsebenzi we-exonuclease, ngaphandle komsebenzi we-3′ →5′ we-exonuclease.

■ Ikhithi ixutshwe ngaphambili ne-Electrophoresis Loading Buffer, ngemva kokuba i-PCR isiqediwe, isampula ingalayishwa ngokuqondile i-electrophoresis;ukusebenza kulula futhi kulula.

Isicelo sekhithi

I-PCR yokukhulisa izingcezu ze-DNA, ukulebula i-DNA, ukulandelana kwe-DNA,I-PCR kanye nomsila we-A

Isitoreji kanye Nempilo yeshelufu

Ingagcinwa isikhathi eside ku -20°C;ukuze isetshenziswe njalo, ingagcinwa ku-4°C isikhashana.Sikholelwa kokuthi: Ukuqamba okusha kuwumphefumulo nomoya wethu.Izinga eliphezulu liyimpilo yethu.Isidingo somthengi nguNkulunkulu wethu ngentengo ecashuniwe ye-Total Rna Reverse Transcription Kit Mirna First Strand Cdna Synthesis Kit Tailing, Siyakwamukela ukusivakashela nganoma yisiphi isikhathi ukuze usungulwe umshado wenhlangano.
Intengo ecashuniwe yeI-China Total Rna Reverse Transcription kanye ne-Cdna Synthesis Tailing Kit, Kukhona imishini ethuthukisiwe yokukhiqiza & yokucubungula kanye nabasebenzi abanamakhono ukuze baqinisekise izinto ngekhwalithi ephezulu.Sithole isevisi enhle kakhulu yangaphambi kokuthengisa, yokuthengisa, yangemuva kokuthengisa ukuze siqinisekise amakhasimende angaqiniseka ukwenza ama-oda.Kuze kube manje izixazululo zethu manje zihamba ngokushesha futhi zithandwa kakhulu eNingizimu Melika, eMpumalanga Asia, eMpumalanga Ephakathi, e-Afrika, njll.