Eminyakeni embalwa edlule, inkampani yethu yamunca futhi yagaya ubuchwepheshe obuphambili ekhaya naphesheya.Ngaleso sikhathi, inkampani yethu inethimba lochwepheshe elizinikele ekuthuthukisweni Kwekhwalithi Enhle I-CE FDA Approved Nucleic Acid Universal DNA Isolation Kit ye-PCR Real Time Detection, Thina, ngothando olukhulu nokwethembeka, sikulungele ukukunikeza izisombululo ezingcono kakhulu futhi siqhubekela phambili nawe ukuze wenze amandla acwebezelayo.
Eminyakeni embalwa edlule, inkampani yethu yamunca futhi yagaya ubuchwepheshe obuphambili ekhaya naphesheya.Khonamanjalo, inkampani yethu izintonga ithimba ochwepheshe abazinikele ekuthuthukiseniI-China Nucleic Acid Isolation Kit kanye neViral Nucleic Acid Isolation, Ngaphandle kwalokho kukhona ukukhiqiza nokuphatha okuphrofeshinali, imishini yokukhiqiza ethuthukisiwe yokuqinisekisa ikhwalithi yethu nesikhathi sokulethwa, inkampani yethu iphishekela isimiso sokholo oluhle, izinga eliphezulu kanye nokusebenza kahle okuphezulu.Siyaqinisekisa ukuthi inkampani yethu izozama konke okusemandleni ethu ukunciphisa izindleko zokuthenga amakhasimende, ukunciphisa isikhathi sokuthenga, ikhwalithi yemikhiqizo ezinzile, ikhulise ukwaneliseka kwamakhasimende futhi izuze isimo sokuwina.

Izincazelo zekhithi

Le khithi isebenzisa ikholomu ye-DNA kuphela engabopha ngokuqondile i-DNA, i-Foregene protease kanye nesistimu yebhafa eyingqayizivele.I-DNA yezinga eliphezulu ye-genomic ingakhishwa kumaseli akhulisiwe ahlukahlukene kanye nezicubu zezilwane kungakapheli imizuzu engama-30 kuye kwengama-50.

Ulwelwesi lwejeli ye-silica ye-DNA kuphela esetshenziswa kukholamu yokuphotha iyinto entsha eyingqayizivele ye-Foregene, engabopha ngempumelelo nangokukhethekile ku-DNA, futhi ikhulise ukukhishwa kwe-RNA, amaprotheni angcolile, ama-ion nezinye izinhlanganisela eziphilayo kumaseli.I-5-80μg yekhwalithi ephezulu ye-genomic DNA ingahlanzwa kusuka ku-10-50mg izicubu.

Imininingwane

*: I-Buffer L2 kanye ne-Buffer PW iqukethe usawoti omuncu ocikayo.Sicela ugqoke amagilavu ​​futhi uthathe izinyathelo zokuzivikela ezifanele lapho usebenza.

Izici&izinzuzo

■ Akukho ukungcoliswa kwe-RNase: Ikholomu Ye-DNA Kuphela enikezwe kukhithi yenza kube nokwenzeka ukususa i-RNA ku-DNA ye-genomic ngaphandle kwe-RNase eyengeziwe phakathi nokuhlolwa, ngaleyo ndlela ivimbele ilabhorethri ukuthi ingangcoliswa i-RNase yangaphandle.

■ Isivinini esisheshayo: I-Foregene Protease inomsebenzi ophezulu kunama-protease afanayo futhi igaya amasampula ezicubu ngokushesha.

■ Okulula : ukusebenza kwe-genomic DNA extraction kungaqedwa ngemizuzu engama-50.

■ Ilula: I-centrifugation yenziwa ekamelweni lokushisa, akukho 4℃ lokushisa eliphansi centrifugation noma ethanol precipitation of DNA.

■ Ukuphepha: asikho i-reagent ephilayo edingekayo.

■ Ikhwalithi ephezulu: I-DNA ye-genomic ekhishiwe inezicucu ezinkulu, ayikho i-RNA, ayikho i-RNase, nokuqukethwe kwe-ion ephansi kakhulu, kungahlangabezana nezidingo zokuhlola okuhlukahlukene.

Isicelo sekhithi

Ilungele ukukhishwa kanye nokuhlanzwa kwe-DNA ye-genomic kusuka kumaseli akhulisiwe amasha noma afriziwe kanye nezicubu zezilwane.

Ukugeleza komsebenzi

Umdwebo

Isitoreji nempilo yeshelufu

Ikhithi ingagcinwa izinyanga eziyi-12 ekamelweni lokushisa (15-25 ℃) kanye no-2-8 ℃ isikhathi eside.

Eminyakeni embalwa edlule, inkampani yethu yamunca futhi yagaya ubuchwepheshe obuphambili ekhaya naphesheya.Ngaleso sikhathi, inkampani yethu inethimba lochwepheshe elizinikele ekuthuthukisweni Kwekhwalithi Enhle I-CE FDA Approved Nucleic Acid Universal DNA Isolation Kit ye-PCR Real Time Detection, Thina, ngothando olukhulu nokwethembeka, sikulungele ukukunikeza izisombululo ezingcono kakhulu futhi siqhubekela phambili nawe ukuze wenze amandla acwebezelayo.
Ikhwalithi EnhleI-China Nucleic Acid Isolation Kit kanye neViral Nucleic Acid Isolation, Ngaphandle kwalokho kukhona ukukhiqiza nokuphatha okuphrofeshinali, imishini yokukhiqiza ethuthukisiwe yokuqinisekisa ikhwalithi yethu nesikhathi sokulethwa, inkampani yethu iphishekela isimiso sokholo oluhle, izinga eliphezulu kanye nokusebenza kahle okuphezulu.Siyaqinisekisa ukuthi inkampani yethu izozama konke okusemandleni ethu ukunciphisa izindleko zokuthenga amakhasimende, ukunciphisa isikhathi sokuthenga, ikhwalithi yemikhiqizo ezinzile, ikhulise ukwaneliseka kwamakhasimende futhi izuze isimo sokuwina.