Ikhithi ye-ForeSNP Genotyping
Incazelo yekhithi:
Ukunemba okuphezulu: ukuthayipha isilawuli esihle, izinga lokunemba lingaphezu kwama-98%.
Izindleko eziphansi: Asikho isidingo sokuhlanganisa inani elikhulu lama-probe abizayo anamalebula amabili.
Uhlelo lwe-PCR olulungiselelwe: ukuzinza okuhle kwesistimu nokucaciswa okuqinile kokukhulisa.
I-anti-pollution PCR system: Ingaqeda ngempumelelo ukungcoliswa kwe-aerosol okubangelwa imikhiqizo ye-PCR, ngaphandle kokukhathazeka ngokuphazanyiswa kokungcoliswa kwemvelo kusiginali ye-fluorescent yokulawula okungalungile, futhi iqinisekise ukucaciswa kokukhulisa kanye nokunemba kokuthayipha.
Imininingwane
Ubuchwepheshe be-Allele Specific PCR (Competitive Allele Specific PCR) buwuhlobo olusha lwendlela yokuthayipha ye-allele.Le ndlela ayidingi ukuhlanganisa ama-probe athile ku-SNP ngayinye ne-inDel, kodwa idinga kuphela amapheya amabili okuhlola kwendawo yonke ehlukile ukuze kuzuzwe ukuthayipha okunembile kwamasampuli e-genomic DNA.Ngokuhlaziya ukuqina nesilinganiso sesiginali yokugcina ye-fluorescence, i-genotype inqunywa ngokuzenzakalelayo, futhi umphumela wokuhlanganisa uboniswa ngokubonakalayo.Le ndlela inesikhathi esifushane sokutholwa, izindleko eziphansi ze-reagent, ukunemba okuphezulu kokutholwa, futhi ingasetshenziselwa ukuzalanisa okusizwa umaka wamangqamuzana, ukuma kwe-QTL, ukuhlonza umaka wofuzo kanye nolunye ucwaningo lwebhayoloji yamangqamuzana anevolumu enkulu yesampula.
Imininingwane
5ml, 50ml, 50ml×10, 500ml×20
Izingxenye zekhithi
| 2× GT EasyTMHlanganisa |
| I-DNase-Free ddH2O |
| Iziyalezo |
Izici&izinzuzo
■Ukunemba okuphezulu: ukuthayipha isilawuli esihle, izinga lokunemba lingaphezu kwama-98%.
■ Izindleko eziphansi: Asikho isidingo sokuhlanganisa inani elikhulu lama-probe abizayo anamalebula amabili.
■ Uhlelo lwe-PCR oluthuthukisiwe: ukuzinza okuhle kwesistimu kanye nokucaciswa okuqinile kokukhulisa umsindo.
■ Uhlelo lwe-PCR olulwa nokungcola: Lungaqeda ngempumelelo ukungcoliswa kwe-aerosol okubangelwa imikhiqizo ye-PCR, ngaphandle kokukhathazeka ngokuphazanyiswa kokungcoliswa kwemvelo kusignali ye-fluorescent yokulawula okungalungile, futhi iqinisekise ukucaciswa kokukhulisa kanye nokunemba kokuthayipha.
Ukusetshenziswa kwekhithi
Ihloselwe ukusetshenziswa ekuhloleni i-genotyping yamasampuli e-DNA ahlanzekile.
Isibonelo
Kulesi sivivinyo, i-200ng wheat genomic DNA isetshenziswe njengesifanekiso ukuze kutholwe ukuthayipha kofuzo lwe-TaGS-D1 oluhlobene nesisindo sokusanhlamvu sikakolweni ngaphansi kwe-Foresnp Genotyping Kit.Imodeli yensimbi yi-BIO-RAD CFX xhuma;inani lamasampula ngu-21, inani lama-NTC ngu-8, futhi uhlobo ngalunye lokulawula okuhle lu-1.
Ikhithi ye-ForeSNP Genotyping
