Sikholelwa kulokhu: Ukuqamba okusha kungumphefumulo nomoya wethu.Ikhwalithi ephezulu impilo yethu.Isidingo somthengi nguNkulunkulu wethu womthombo Wefekthri I-China CE/FDA Igunyazwe Ubuhlalu Bezibuthe Indlela I-DNA / I-Rna Nucleic Acid Extraction Regent Kits (Ubuhlalu obububuthe), Qiniseka ukuthi uzizwe ukhululekile ukuxhumana nathi nganoma yisiphi isikhathi.Sizokuphendula uma sithola imibuzo yakho.Sicela uqaphele ukuthi amasampula ayatholakala ngaphambi kokuthi siqale ibhizinisi lethu.
Sikholelwa kulokhu: Ukuqamba okusha kungumphefumulo nomoya wethu.Ikhwalithi ephezulu impilo yethu.Isidingo somthengi singuNkulunkulu wethuI-China Nucleic Acid, China Nucleic Acid Manufacturers, Ngaphandle kwamandla aqinile wezobuchwepheshe, siphinde sethule imishini ethuthukisiwe ukuze ihlolwe futhi siphathe ukuphathwa okuqinile.Bonke abasebenzi benkampani yethu bamukela abangani ekhaya nakwamanye amazwe ukuthi beze ukuzovakashelwa namabhizinisi ngesisekelo sokulingana nokuhlomula ngakho konke.Uma unentshisekelo kunoma yiziphi izinto zethu, khumbula ukuthi ukhululeke ukuxhumana nathi ukuze uthole ikhotheshini kanye nemininingwane yomkhiqizo.

Kits Izincazelo

50 Preps, 200 Preps

Le kit isebenzisa ikholomu ye-spin kanye nefomula eyakhiwe inkampani yethu, engakwazi ukukhipha i-RNA ephezulu kanye nekhwalithi ephezulu engqikithini yezilwane ezihlukahlukene ngokusebenza kahle okuphezulu.Ihlinzeka ngekholomu ye-DNA-Cleaning ephumelelayo, engakwazi ukuhlukanisa kalula futhi ikhangise i-DNA ye-genomic ku-supernatant kanye ne-tissue lysate, elula futhi elondoloza isikhathi;Ikholomu ye-RNA kuphela ingahlanganisa ngempumelelo i-RNA futhi ingacutshungulwa kanyekanye ngefomula eyingqayizivele Amasampuli amaningi.

Lonke uhlelo luyi-RNase-Free, ukuze i-RNA ekhishiwe ingonakalisiwe;I-Buffer RW1, i-Buffer RW2 isistimu yokugeza ibhafa, ukuze i-RNA etholiwe ingabi nawo amaprotheni, i-DNA, i-ion, kanye nokungcoliswa kwenhlanganisela yezinto eziphilayo.

Izingxenye zekhithi:

Izici&izinzuzo

■ Asikho isidingo sokukhathazeka ngokuwohloka kwe-RNA;lonke uhlelo luyi-RNase-Free
■ Khipha ngempumelelo i-DNA usebenzisa i-DNA-Cleaning Column
■ Khipha i-DNA ngaphandle kokwengeza i-DNase
■ Kulula-yonke imisebenzi iqedwa ekamelweni lokushisa
■ Ukusebenza okusheshayo kungaqedwa ngemizuzu engama-30
■ I-Safe-akukho reagent ephilayo edingekayo
■ Ukuhlanzeka okuphezulu -OD260/280≈1.8-2.1

Isicelo sekhithi

Ilungele ukukhishwa nokuhlanzwa kwe-RNA ephelele ezinhlobonhlobo zezicubu zezilwane ezintsha noma eziqandisiwe noma amaseli akhulisiwe.

Imingcele yomkhiqizo

■ Izinhlelo zokusebenza eziwela phansi: ukuhlanganiswa kwe-cDNA ye-first-strand, i-RT-PCR, i-molecular cloning, i-Northern Blot, njll.
■ Amasampula: izicubu zezilwane, amaseli akhulisiwe
■ Umthamo: Izicubu 10-20mg, Amaseli(2-5)×10⁶
■ Umthamo omkhulu wokubopha we-DNA wekholomu yokuhlanza: 80 μg
■ Ivolumu ye-Elution: 50-200 μl

Ukugeleza komsebenzi

Umdwebo

I-Animal Total RNA Isolation Kit iphathwe ama-20mg
Amasampula egundane amasha, thatha i-RNA ephelele engu-5% 1% ye-agar

I-Glycogel electrophoresis
1: Ubende 2: Izinso
3: Isibindi 4: Inhliziyo

Isitoreji nempilo yeshelufu

Ikhithi ingagcinwa izinyanga ezingama-24 ekamelweni lokushisa (15-25 ℃) noma 2–8 ℃ isikhathi eside.I-Buffer RL1 ingagcinwa ku-4 ℃ inyanga engu-1 ngemva kokwengeza i-β- mercaptoethanol (uma uthanda).

Sikholelwa kulokhu: Ukuqamba okusha kungumphefumulo nomoya wethu.Ikhwalithi ephezulu impilo yethu.Isidingo somthengi nguNkulunkulu wethu womthombo Wefekthri I-China CE/FDA Igunyazwe Ubuhlalu Bezibuthe Indlela I-DNA / I-Rna Nucleic Acid Extraction Regent Kits (Ubuhlalu obububuthe), Qiniseka ukuthi uzizwe ukhululekile ukuxhumana nathi nganoma yisiphi isikhathi.Sizokuphendula uma sithola imibuzo yakho.Sicela uqaphele ukuthi amasampula ayatholakala ngaphambi kokuthi siqale ibhizinisi lethu.
Umthombo wefekthriI-China Nucleic Acid, China Nucleic Acid Manufacturers, Ngaphandle kwamandla aqinile wezobuchwepheshe, siphinde sethule imishini ethuthukisiwe ukuze ihlolwe futhi siphathe ukuphathwa okuqinile.Bonke abasebenzi benkampani yethu bamukela abangani ekhaya nakwamanye amazwe ukuthi beze ukuzovakashelwa namabhizinisi ngesisekelo sokulingana nokuhlomula ngakho konke.Uma unentshisekelo kunoma yiziphi izinto zethu, khumbula ukuthi ukhululeke ukuxhumana nathi ukuze uthole ikhotheshini kanye nemininingwane yomkhiqizo.