Sisebenzisa uhlelo oluphelele lwesayensi lokuphatha ikhwalithi ephezulu, ikhwalithi ephezulu kanye nokholo oluphakeme, sithola idumela elihle futhi sathatha le mboniIkhwalithi ephezulu ye-RNA, Imfundiso yethu ithi “Amanani aphansi, isikhathi sokukhiqiza esiyongayo kanye nenkonzo engcono kakhulu” Sithemba ukubambisana nabathengi abengeziwe ukuze sithuthukisane futhi sizuze.
Sisebenzisa uhlelo oluphelele lwesayensi lokuphatha ikhwalithi ephezulu, ikhwalithi ephezulu kanye nokholo oluphakeme, sithola idumela elihle futhi sathatha le mboniIkhwalithi ephezulu ye-RNA, Inkampani yethu ibheka "izintengo ezifanele, ikhwalithi ephezulu, isikhathi sokukhiqiza esisebenzayo kanye nensizakalo enhle yangemva kokuthengisa" njengemfundiso yethu.Sithemba ukubambisana namakhasimende amaningi ukuze sithuthuke futhi sizuze esikhathini esizayo.Siyakwamukela ukuxhumana nathi.

Imininingwane

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Izingxenye zekhithi

Izici&izinzuzo

■ Yonke inqubo iqhutshwa ekamelweni lokushisa (15-25 ℃), ngaphandle kokugeza kweqhwa kanye nokushisa okuphansi kwe-centrifugation.
■ Ikhithi yonke ayinayo i-RNase-Free, asikho isidingo sokukhathazeka ngokuwohloka kwe-RNA.
■ Ikholomu Yokuhlanza I-DNA ibopha ngokukhethekile i-DNA, ukuze ikhithi ikwazi ukususa ukungcola kwe-DNA ye-genomic ngaphandle kokwengeza i-DNase eyengeziwe.
■ Isivuno esikhulu se-RNA: Ikholomu ye-RNA kuphela kanye nefomula eyingqayizivele ingahlanza i-RNA ngokuphumelelayo.
■ Isivinini esisheshayo: kulula ukusebenza futhi singaqedwa ngemizuzu eyi-11.
■ Ukuphepha: Asikho i-reagent ephilayo edingekayo.
■ Ikhwalithi ephezulu: I-RNA ehlanziwe imsulwa kakhulu, ayinamaprotheni nokunye ukungcola, futhi ingahlangabezana nokuhlola okuhlukahlukene okulandelayo.

Isicelo sekhithi

Ilungele ukukhishwa nokuhlanzwa kwe-RNA ephelele kumaseli akhulisiwe kumapuleti angama-96, 24, 12, kanye namapuleti angu-6.

Ukugeleza komsebenzi

Umdwebo

Umdwebo webhethri yejeli ye-agarose ye-Cell Total RNA Isolation Kit iphathe izinombolo ezingenhla ezihlukene zamaseli, ukukhishwa kwevolumu engu-20μl, thatha u-2μl isamba esihlanziwe se-RNA 1%.

Isitoreji nempilo yeshelufu

Ikhithi ingagcinwa izinyanga eziyi-12 ekamelweni lokushisa (15-25 ℃) kanye no-2-8 ℃ isikhathi eside (izinyanga ezingu-24).

I-Buffer cRL1 ingagcinwa ku-4 ℃ inyanga engu-1 ngemva kokwengeza i-2-hydroxy-1-ethanethiol(uyazikhethela). Sisebenzisa uhlelo oluphelele lwesayensi lokuphatha izinga eliphezulu, izinga eliphezulu nokholo oluphakeme, sizuza idumela elihle futhi sasebenzisa le mboni Yekhwalithi Ephezulu yaseChina Longyu Nikeza I-Nomilin Yekhwalithi Ethembekile, Umgomo wethu uthi “Inzuzo Engcono Kakhulu yokukhiqiza” al ukuthuthukiswa kanye nezinzuzo.
Ikhwalithi ephezulu ye-China Nomilin, i-Solvent Extraction, Inkampani yethu ibheka "izintengo ezifanele, ikhwalithi ephezulu, isikhathi esisebenza kahle sokukhiqiza kanye nenkonzo enhle yangemva kokuthengisa" njengemfundiso yethu.Sithemba ukubambisana namakhasimende amaningi ukuze sithuthuke futhi sizuze esikhathini esizayo.Siyakwamukela ukuxhumana nathi.