p53/D13S319 Ukuhlolwa Kombala Okabili
Incazelo yekhithi:
I-Fluorescence in situ hybridization (FISH) isuselwe kumgomo wokumataniswa okuhambisanayo kwezisekelo ze-DNA, kanye nokubonwa kwamasiginali we-hybridization we-DNA probes enelebuli ye-fluorescence nokulandelana kwayo okuqondiwe kwe-DNA ku-nucleus yeseli ngaphansi kwesibonakhulu se-fluorescence.
Incazelo
I-Fluorescence in situ hybridization (FISH) isuselwe kumgomo wokumataniswa okuhambisanayo kwezisekelo ze-DNA, kanye nokubonwa kwamasiginali we-hybridization we-DNA probes enelebuli ye-fluorescence nokulandelana kwayo okuqondiwe kwe-DNA ku-nucleus yeseli ngaphansi kwesibonakhulu se-fluorescence.
Izingxenye zekhithi
| Isakhi | Imininingwane | ||
| 5Izivivinyo | 10Izivivinyo | 20Izivivinyo | |
| p53/D13S319 Ukuhlolwa Kombala Okabili | 50μl | 100μl | 200μl |
Isicelo sekhithi
Le khithi isetshenziselwa ukuthola ngokwekhwalithi ukungavamile kofuzo lomuntu lwe-p53 kanye ne-D13S319 kumasampuli e-cytological in vitro.
Izidingo zesifanekiso
Ukuze uthole amasampula egazi amathambo noma angaphandle, amasampula amasha angalungisiwe kufanele agcinwe ku-4°C amahora angeqile kwangu-24, futhi ukumiswa kwamaseli okugxilile kufanele kugcinwe ku -20°C.
Isitoreji kanye Nempilo yeshelufu
Igcinwe ku -20℃±5℃ ebumnyameni, isebenza izinyanga eziyi-12.Ezokuthutha ngaphansi kuka-8 ℃.
