I-China China Umhlinzeki Wegolide Wesikhathi Sangempela Se-Fluorescence Ikhithi Yokuhlola I-Rt-PCR Yomkhiqizi Nomhlinzeki Wokuhlola I-Nucleic Acid |I-Foregene

Umhlinzeki Wegolide wase-China we-Fluorescence Real Time Rt-PCR Test Reagent Kit yokuhlolwa kwe-Nucleic Acid

Umhlinzeki Wegolide wase-China we-Fluorescence Real Time Rt-PCR Test Reagent Kit yokuhlola i-Nucleic Acid Isithombe Esifakiwe

Incazelo yekhithi:

I-Simple—2X PCR Mix ukuze kwehliswe iphutha lokuhlola nesikhathi sokusebenza

I-Specific-optimized buffer kanye ne-hot-start Taq enzyme ingavimbela ukukhulisa okungaqondile kanye nokwakheka kwe-primer dimer

Ukuzwela okuphezulu-kungathola amakhophi aphansi esifanekiso

Ukuguquguquka okuhle—kuhambisana nezinsimbi eziningi zesikhathi sangempela ze-PCR

Thumela i-imeyili kithi Landa njenge-PDF

Imininingwane Yomkhiqizo

Omaka bomkhiqizo

Sicabanga lokho abathengi abakucabangayo, ukuphuthuma kokuphuthuma kokuthatha isinyathelo ngesikhathi semibono yomthengi, okuvumela izinga eliphezulu kakhulu, izindleko ezincishisiwe zokucubungula, izindleko zinengqondo kakhudlwana, kuzuze abathengi abasha nabaphelelwe yisikhathi ukusekelwa nokuqinisekiswa kwe-China Gold Supplier ye-Fluorescence Real Time Rt-PCR izinzuzo ze-Reagent Eyengeziwe Engxenyeni Yezivivinyo Ezitholakalayo ukusondela esikhathini esizayo.
Sicabanga lokho abathengi abakucabangayo, ukuphuthuma kokuphuthuma kokuthatha isinyathelo ngesikhathi sokuthakasela isikhundla somthengi sethiyori, okuvumela izinga eliphezulu kakhulu, izindleko ezincishisiwe zokucubungula, izindleko zinengqondo kakhudlwana, kuzuze abathengi abasha nabaphelelwe yisikhathi ukusekelwa nokuqinisekiswa.Ikhithi ye-PCR Reagent yase-China kanye nokuhlolwa kwe-Rt-PCR, Ngomgomo othi "zero defect".Ukunakekela indawo ezungezile, kanye nembuyiselo yenhlalo, nakekela isibopho somphakathi somsebenzi njengomsebenzi waso.Samukela abangani abavela emhlabeni wonke ukuthi basivakashele futhi basiqondise ukuze sifinyelele umgomo wokunqoba ndawonye.

Izincazelo zekhithi

I-2×Real PCR Easy^TMI-Mix-SYBR ihlinzekwa yi-Real Time PCR Easy^TM-Ikhithi ye-SYBR Green I iwuhlelo olusha lwe-premix olusebenzisa i-SYBR Green I ye-Real Time PCR ukusabela kokukhulisa, okungathuthukisa kakhulu ukucaciswa komkhiqizo nokuzwela kokusabela .I-ROX inikezwa njengodayi wokulawula wangaphakathi.Amandla e-fluorescence ale khithi aphindwe izikhathi ezingu-3-5 kunemikhiqizo efanayo, engazwela kakhulu futhi ibonise ngokunembile ukugxila kwesifanekiso se-DNA esihlosiwe.

2×I-PCR Yangempela Kulula^TMI-Mix-SYBR iqukethe i-Taq DNA Polymerase eyingqayizivele ye-Foregene.Uma iqhathaniswa nama-enzyme e-Taq ajwayelekile, inezinzuzo zokusebenza kahle kokukhulisa i-amplification, ikhono elithile eliqinile lokukhulisa kanye nezinga lokungafani eliphansi.Inganciphisa ukukhulisa okungaqondile futhi ithuthukise ukunemba kwe-PCR.

Imininingwane

Isikhathi Sangempela PCR Easyᵀᴹ-SYBR Green I Kit
Ukwakheka kwekhithi(20μl system)	QP-01011	QP-01012	QP-01013	QP-01014
Ukwakheka kwekhithi(20μl system)	200T	500T	1000T	2000T
I-2 × I-PCR yangempela kulula^TMI-Mix-SYBR	1 ml × 2	1.7 ml × 3	1.7 ml × 6	1.7 ml × 12
I-50 × I-ROX Reference Dye	400 μl	1 ml	1 ml × 2	1 ml × 4
I-DNase-Free ddH₂O	1.7 ml	1.7 ml × 2	10 ml	20 ml
Isiyalezo	1	1	1	1

Izici&izinzuzo

■ I-Simple—2X PCR Mix ukuze kwehliswe iphutha lokuhlola nesikhathi sokusebenza

■ Ibhafa ecacisiwe—elungiselelwe kahle kanye ne-enzyme ye-Taq eqala ukushisa ingavimbela ukukhulisa okungaqondile kanye nokwakheka kwe-primer dimer.

■ Ukuzwela okuphezulu—kukwazi ukubona amakhophi aphansi esifanekiso

■ Ukuguquguquka okuhle—kuhambisana nezinsimbi eziningi zesikhathi sangempela ze-PCR

Isicelo sekhithi

Ukuhlaziywa kwe-qPCR

Ukugeleza komsebenzi

I-RT PCR-SYBR

Umfanekiso

Isikhathi Sangempela PCR Easy TM SYBR GREEN I GRAPHIC

Isitoreji nempilo yeshelufu

Le kit kufanele igcinwe kude nokukhanya futhi kufanele igcinwe ku -20 ℃.Uma isetshenziswa njalo, ingabuye igcinwe ku-4℃ isikhathi esifushane (izinsuku eziyi-10).

Sicabanga lokho abathengi abakucabangayo, ukuphuthuma kokuphuthuma kokuthatha isinyathelo ngesikhathi semibono yomthengi, okuvumela izinga eliphezulu kakhulu, izindleko ezincishisiwe zokucubungula, izindleko zinengqondo kakhudlwana, kuzuze abathengi abasha nabaphelelwe yisikhathi ukusekelwa nokuqinisekiswa kwe-China Gold Supplier ye-Fluorescence Real Time Rt-PCR izinzuzo ze-Reagent Eyengeziwe Engxenyeni Yezivivinyo Ezitholakalayo ukusondela esikhathini esizayo.
China Gold Supplier forIkhithi ye-PCR Reagent yase-China kanye nokuhlolwa kwe-Rt-PCR, Ngomgomo othi "zero defect".Ukunakekela indawo ezungezile, kanye nembuyiselo yenhlalo, nakekela isibopho somphakathi somsebenzi njengomsebenzi waso.Samukela abangani abavela emhlabeni wonke ukuthi basivakashele futhi basiqondise ukuze sifinyelele umgomo wokunqoba ndawonye.

Okwedlule: Isampula yamahhala yasefekthri ye-ODM Cell Yamahhala Ye-DNA Isolation Kit Yocwaningo Lwebhayoloji Yamangqamuzana

Olandelayo: Izitolo zefekthri zeVirus Magnetic Bead Method Rna-DNA Nucleic Acid 96 Isampula Yekhithi Yokuhlanzwa Kwethubhu

Bhala umyalezo wakho lapha futhi usithumelele wona