Sikholelwa ukuthi ubambiswano olude empeleni luwumphumela wekhwalithi ephezulu, izinsizakalo ezibizayo, isipiliyoni esiphumelelayo kanye nokuxhumana komuntu siqu kwefekthri Izitolo zeVirus Magnetic Bead Method Rna-DNA Nucleic Acid 96 Sample Extraction Purification Tube Kit, Sinomuzwa wokuthi izisebenzi ezishisekayo, ezisebenza kanzima futhi eziqeqeshwe kahle zingakha izinhlangano zebhizinisi ezinhle kakhulu neziwusizo ngokufanayo nawe.Qiniseka ukuthi uzizwa ukhululekile ngokuphelele ukuxhumana nathi ukuze uthole imininingwane eyengeziwe.
Sikholelwa ukuthi ubambiswano olude empeleni luwumphumela wekhwalithi ephezulu, izinsiza ezidingekayo, ulwazi oluphumelelayo kanye nokuxhumana komuntu siquIkhithi yokukhipha i-Acid yaseChina (i-rna-DNA) kanye ne-96 Nucleic Acid, Siphikelela ekuphathweni komugqa wokukhiqiza wekhwalithi ephezulu kanye nosizo lochwepheshe lwamakhasimende, manje sesiklame ukulungiswa kwethu ukuze sinikeze abathengi bethu sisebenzisa isipiliyoni esisebenzayo sokuthola inani nangemva nje kwezinsizakalo.Ukugcina ubudlelwano obunobungane obukhona nabathengi bethu, nokho sisungula uhlu lwethu lwezixazululo ngaso sonke isikhathi ukuze sanelise izimfuno ezintsha futhi sihambisane nentuthuko yakamuva kakhulu yemakethe eMalta.Sikulungele ukubhekana nokukhathazeka futhi senze ngcono ukuqonda wonke amathuba ekuhwebeni kwamazwe ngamazwe.

Imininingwane

50 Preps, for genomic DNA purification egazini lonke kusetshenziswa igazi elingu<1ml

I-Buffer DC: Ihambisa amaseli abomvu egazi egazini.

I-Buffer BL1: Nikeza ngendawo yokugaya i-enzymatic eyisampula.

I-Foregene Protease Plus: Gaya amasampula egazi nge-enzyme endaweni ye-Buffer BL1.

I-Buffer BL2: Yenza i-Foregene Protease Plus isebenze futhi inikeze indawo yokulayisha i-DNA.

I-Buffer PW: Susa ukungcola okufana namaprotheni ne-RNA ku-DNA.

I-Buffer WB1: Susa ama-ion kasawoti asele ku-DNA.

I-Buffer EB: I-Elute i-DNA kulwelwesi lwekholomu yokuhlanza.

Ikholomu Ye-DNA Kuphela: Ikhangisa i-DNA ye-genomic ku-lysate.

Izingxenye zekhithi

Izici&izinzuzo

-Akukho ukungcola kwe-RNase: Ikholomu Ye-DNA Kuphela ehlinzekwa ikhithi yenza kube nokwenzeka ukususa i-RNA ku-DNA ye-genomic ngaphandle kokwengeza i-RNase phakathi nokuhlolwa, ukugwema ilabhorethri ukuthi ingangcoliswa i-RNase yangaphandle.

-Isivinini esisheshayo: I-Foregene Protease Plus inomsebenzi ophezulu kunama-protease afanayo, futhi igaya amasampula ezicubu ngokushesha;ukusebenza kulula, futhi ukusebenza kwe-genomic DNA extraction kungaqedwa phakathi nemizuzu engama-40.

-Okulula: I-centrifugation yenziwa ekamelweni lokushisa, i-4°C centrifugation noma i-ethanol precipitation ye-DNA ayidingeki.

-Ukuphepha: Akukho kukhishwa kwe-organic reagent esetshenziswayo.

-Ikhwalithi ephezulu: Izingcezu ze-genomic DNA ezikhishiwe zinkulu, azikho i-RNA, azinayo i-RNase, nokuqukethwe kwe-ion okuphansi kakhulu, okungahlangabezana nezidingo zokuhlolwa okuhlukahlukene.

Isicelo sekhithi

Ilungele ukukhishwa kanye nokuhlanzwa kwe-genomic DNA egazini elisha noma eliqandisiwe elilwa nokuqunjelwa.

Ukuhamba komsebenzi

Isitoreji kanye Nempilo yeshelufu

Le kit ingagcinwa izinyanga ezingu-12 ngaphansi kwezimo ezomile ekamelweni lokushisa (15-25 ° C);uma idinga ukugcinwa isikhathi eside, ingagcinwa ku-2-8°C.

Qaphela: Uma igcinwe ezingeni lokushisa eliphansi, isixazululo sithambekele ekubeni nemvula.Ngaphambi kokusetshenziswa, qiniseka ukuthi ubeka ikhambi kukhithi ekamelweni lokushisa isikhathi esithile.Uma kunesidingo, yishise ngaphambili emanzini okugeza angu-37 ° C imizuzu engu-10 ukuze uhlakaze i-precipitate, futhi uyixube ngaphambi kokusetshenziswa.

Isixazululo se-Foregene Protease Plus sinefomula eyingqayizivele, esebenzayo uma igcinwe ekamelweni lokushisa isikhathi eside (izinyanga ezi-3);izosebenza kakhulu futhi izinzile uma igcinwe ku-4 ° C, ngakho-ke kunconywa ukuyigcina ku-4 ° C, khumbula ukuthi ungayigcini ku -20 ° C. Sikholelwa ukuthi ukubambisana okude kwenkulumo empeleni kuwumphumela wekhwalithi ephezulu, izinsizakalo ezingeziwe, isipiliyoni esiphumelelayo kanye nokuxhumana komuntu siqu kwefekthri Izitolo zeVirus Magnetic Bead Indlela ye-Rna-DNA Nucleic Acid , I-Purification I-Purification Iphuzu le-Sampuli umsebenzi we-96 we-Purification amandla angakha izinhlangano zebhizinisi ezinhle neziwusizo ngokufanayo nawe ngokushesha.Qiniseka ukuthi uzizwa ukhululekile ngokuphelele ukuxhumana nathi ukuze uthole imininingwane eyengeziwe.
Izitolo zemboni zeIkhithi yokukhipha i-Acid yaseChina (i-rna-DNA) kanye ne-96 Nucleic Acid, Siphikelela ekuphathweni komugqa wokukhiqiza wekhwalithi ephezulu kanye nosizo lochwepheshe lwamakhasimende, manje sesiklame ukulungiswa kwethu ukuze sinikeze abathengi bethu sisebenzisa isipiliyoni esisebenzayo sokuthola inani nangemva nje kwezinsizakalo.Ukugcina ubudlelwano obunobungane obukhona nabathengi bethu, nokho sisungula uhlu lwethu lwezixazululo ngaso sonke isikhathi ukuze sanelise izimfuno ezintsha futhi sihambisane nentuthuko yakamuva kakhulu yemakethe eMalta.Sikulungele ukubhekana nokukhathazeka futhi senze ngcono ukuqonda wonke amathuba ekuhwebeni kwamazwe ngamazwe.