Sinabasebenzi bethu abathengisa imikhiqizo, abasebenzi besitayela, iqembu lobuchwepheshe, abasebenzi be-QC nabasebenzi bephakheji.Manje sinezinqubo eziqinile zokuphatha izinga eliphezulu zendlela ngayinye.Futhi, bonke abasebenzi bethu banolwazi esihlokweni sokuphrinta se-OEM/ODM ManufacturerI-China Nucleic Acid Extraction Reagent(Isixazululo se-Serum/ Plasma ne-Puncture) , I-Rna Extraction Test Kits, Inhloso yethu yokuvala ithi “Ukuzama okuzuzisa kakhulu, Ukuze ngokujwayelekile kube Okuhamba phambili”.Qiniseka ukuthi uzizwa mahhala ukuze ubambe nathi uma unezidingo ezidingekayo.
Sinabasebenzi bethu abathengisa imikhiqizo, abasebenzi besitayela, iqembu lobuchwepheshe, abasebenzi be-QC nabasebenzi bephakheji.Manje sinezinqubo eziqinile zokuphatha izinga eliphezulu zendlela ngayinye.Futhi, zonke izisebenzi zethu zinolwazi ngesihloko sokuphrintaI-China Nucleic Acid Extraction Reagent, Indlela ye-Magnetic Bead, Imfundiso yethu “iwubuqotho kuqala, ikhwalithi engcono kakhulu”.Manje siyazethemba ekukunikezeni isevisi enhle kakhulu nezinto ezifanele.Sithemba ngobuqotho ukuthi singasungula ukubambisana kwebhizinisi lokuwina-win nawe esikhathini esizayo!

Imininingwane

50 Preps, 200 Preps

Izingxenye zekhithi

Izici&izinzuzo

■ Asikho isidingo sokukhathazeka ngokuwohloka kwe-RNA;lonke uhlelo luyi-RNase-Free
■ Khipha ngempumelelo i-DNA usebenzisa i-DNA-Cleaning Column
■ Khipha i-DNA ngaphandle kokwengeza i-DNase
■ Kulula-yonke imisebenzi iqedwa ekamelweni lokushisa
■ Ukusebenza okusheshayo kungaqedwa ngemizuzu engama-30
■ I-Safe-akukho reagent ephilayo edingekayo
■ Ukuhlanzeka okuphezulu -OD260/280≈1.8-2.1

Isicelo sekhithi

Ilungele ukukhishwa nokuhlanzwa kwe-RNA ephelele ezinhlobonhlobo zezicubu zezilwane ezintsha noma eziqandisiwe noma amaseli akhulisiwe.

Imingcele yomkhiqizo

■ Izinhlelo zokusebenza eziwela phansi: ukuhlanganiswa kwe-cDNA ye-first-strand, i-RT-PCR, i-molecular cloning, i-Northern Blot, njll.
■ Amasampula: izicubu zezilwane, amaseli akhulisiwe
■ Umthamo: Izicubu 10-20mg, Amaseli(2-5)×10⁶
■ Umthamo omkhulu wokubopha we-DNA wekholomu yokuhlanza: 80 μg
■ Ivolumu ye-Elution: 50-200 μl

Ukugeleza komsebenzi

Umdwebo

I-Animal Total RNA Isolation Kit iphathwe ama-20mg
Amasampula egundane amasha, thatha i-RNA ephelele engu-5% 1% ye-agar

I-Glycogel electrophoresis
1: Ubende 2: Izinso
3: Isibindi 4: Inhliziyo

Isitoreji nempilo yeshelufu

Ikhithi ingagcinwa izinyanga ezingama-24 ekamelweni lokushisa (15-25 ℃) noma 2–8 ℃ isikhathi eside.I-Buffer RL1 ingagcinwa ku-4 ℃ inyanga engu-1 ngemva kokwengeza i-β- mercaptoethanol (uyazikhethela).Sinabasebenzi bethu bokuthengisa imikhiqizo, abasebenzi besitayela, iqembu lobuchwepheshe, abasebenzi be-QC nabasebenzi bephakheji.Manje sinezinqubo eziqinile zokuphatha izinga eliphezulu zendlela ngayinye.Futhi, bonke abasebenzi bethu banolwazi esihlokweni sokuphrinta se-OEM/ODM ManufacturerI-China Nucleic Acid Extraction Reagent(Isixazululo se-Serum/ Plasma ne-Puncture) , I-Rna Extraction Test Kits, Inhloso yethu yokuvala ithi “Ukuzama okuzuzisa kakhulu, Ukuze ngokujwayelekile kube Okuhamba phambili”.Qiniseka ukuthi uzizwa mahhala ukuze ubambe nathi uma unezidingo ezidingekayo.
Umkhiqizi we-OEM/ODM waseChina Nucleic Acid Extraction Reagent,Indlela ye-Magnetic Bead, Imfundiso yethu “iwubuqotho kuqala, ikhwalithi engcono kakhulu”.Manje siyazethemba ekukunikezeni isevisi enhle kakhulu nezinto ezifanele.Sithemba ngobuqotho ukuthi singasungula ukubambisana kwebhizinisi lokuwina-win nawe esikhathini esizayo!