Sijabulela igama elihle kakhulu phakathi kwabathengi bethu ngomkhiqizo wethu okhethekile noma isevisi enhle kakhulu, izinga lokuncintisana kanye nezinsizakalo ezinkulu kakhulu ze-OEM China China Isampula Yamahhala 32t 64t Nucleic Acid DNA Rna Extraction and Purification Reagent Kit Yesikhathi Sangempela Ukuxilongwa kwe-PCR Kusuka Kumkhiqizi Woqobo Uni-Medica nge-CE, Imikhiqizo yethu eminingi isetshenziswa ezindaweni eziningi zezimboni.Uphiko Lwezinsizakalo Zenkampani Yethu ngokwethembeka ngenjongo yekhwalithi yokuphila.Konke okwesevisi yamakhasimende.
Sijabulela igama elihle kakhulu phakathi kwabathengi bethu ngomkhiqizo wethu noma isevisi enhle kakhulu, izinga lokuncintisana kanye nezinkonzo ezinhle kakhulu64t Rna Isizinda Reagent, Ikhithi yokukhipha i-DNA yaseChina 64tSekuyiminyaka engaphezu kwengu-20 senza izinto zethu .Ikakhulukazi yenza ama-wholesale, ngakho-ke manje sinenani elincintisana kakhulu, kodwa ikhwalithi ephezulu kakhulu.Eminyakeni edlule , sithole izimpendulo ezinhle kakhulu , hhayi nje ngoba siletha izixazululo ezinhle , kodwa nangenxa yesevisi yethu enhle ngemva kokuthengisa .Silapha silinde wena ukuze uthole uphenyo lwakho.

Imininingwane

50 Preps, 200 Preps

Ikhithi isebenzisa ikholomu yokuphotha kanye nefomula eyakhiwe yi-Foregene, engakhipha ngempumelelo i-RNA ephezulu nekhwalithi ephezulu ezicutshini zezitshalo ezihlukahlukene ezinokuqukethwe okuphansi kwama-polysaccharides nama-polyphenols.Kumasampula ezitshalo ane-polysaccharides ephezulu noma okuqukethwe kwama-polyphenols, kunconyelwa ukusebenzisa i-Plant Total RNA Isolation Plus Kit ukuze uthole imiphumela engcono yokukhipha i-RNA.Ikhithi inikeza ikholomu ye-DNA-Cleaning engasusa kalula i-genomic DNA ku-supernatant kanye ne-tissue lysate.Ikholomu ye-RNA kuphela ingabopha ngempumelelo i-RNA.Ikhithi ingacubungula inani elikhulu lamasampuli ngesikhathi esisodwa.

Lonke uhlelo aluqukethe i-RNase, ngakho-ke i-RNA ehlanziwe ngeke yehliswe.I-Buffer PRW1 kanye ne-Buffer PRW2 ingaqinisekisa ukuthi i-RNA etholiwe ayingcoliswanga amaprotheni, i-DNA, ama-ion, nezinhlanganisela eziphilayo.

Izingxenye zekhithi

Izici&izinzuzo

■ Ukusebenza ekamelweni lokushisa (15-25℃) kuyo yonke inqubo, ngaphandle kokugeza eqhweni nokushisa okuphansi kwe-centrifugation.
■ Ikhithi ephelele ye-RNase-Free, asikho isidingo sokukhathazeka ngokuwohloka kwe-RNA.
■ Ikholomu Yokuhlanza I-DNA ibophezela ngokukhethekile ku-DNA, ukuze ikhithi isuse ukungcoliswa kwe-DNA ye-genomic ngaphandle kokwengeza i-DNase.
■ Isivuno esikhulu se-RNA: Ikholomu ye-RNA kuphela kanye nefomula eyingqayizivele ingahlanza i-RNA ngokuphumelelayo.
■ Isivinini esisheshayo: kulula ukusebenza futhi singaqedwa phakathi nemizuzu engama-30.
■ Ukuphepha: asikho i-reagent ephilayo edingekayo.
■ Ikhwalithi ephezulu: Izingcezwana ze-RNA ezihlanziwe zimsulwa kakhulu, azinawo amaphrotheni nokunye ukungcola, futhi zingahlangabezana nezinhlelo zokuhlola ezihlukahlukene ezansi nomfula.

Isicelo sekhithi

Ilungele ukukhishwa nokuhlanzwa kwe-RNA ephelele kumasampula ezicubu zezitshalo ezintsha noma eziqandisiwe (ikakhulukazi izicubu zamaqabunga ezitshalo ezintsha) ezine-polysaccharide ephansi kanye nokuqukethwe kwe-polyphenol.

Ukugeleza komsebenzi

Umdwebo

I-Plant Total RNA Isolation Kit Plus icutshungulwe u-50mg wamaqabunga amasha e-polysaccharides nama-polyphenols, futhi i-RNA ehlanzekile engu-5% yahlolwa nge-electrophoresis.
1: Ubhanana
2: Ginkgo
3: Ukotini
4: Ihalanathi

Isitoreji nempilo yeshelufu

Ikhithi ingagcinwa izinyanga eziyi-12 ekamelweni lokushisa (15-25 ℃) endaweni eyomile, kanye no-2-8 ℃ isikhathi eside (izinyanga ezingu-24).

I-Buffer PSL1 ingagcinwa ku-4 ℃ inyanga engu-1 ngemva kokwengeza i-2-hydroxy-1-ethanethiol(ongakukhetha). Sijabulela igama elihle kakhulu phakathi kwabathengi bethu ngomkhiqizo wethu okhethekile noma isevisi enhle kakhulu, izinga lokuncintisana kanye nezinkonzo ezinkulu kakhulu ze-OEM China China Isampula Yamahhala 32t 64t Nucleic Acid Ye-DNA Purification Real Ukukhishwa Kwe-PC Ukukhishwa Kwe-PC Real Ukukhishwa kwe-PC ReaniR ReaniR I-Medica ene-CE, Imikhiqizo yethu isetshenziswa kabanzi emikhakheni eminingi yezimboni.Uphiko Lwezinsizakalo Zenkampani Yethu ngokwethembeka ngenjongo yekhwalithi yokuphila.Konke okwesevisi yamakhasimende.
OEM ChinaIkhithi yokukhipha i-DNA yaseChina 64t, 64t Rna Isizinda ReagentSekuyiminyaka engaphezu kwengu-20 senza izinto zethu .Ikakhulukazi yenza ama-wholesale, ngakho-ke manje sinenani elincintisana kakhulu, kodwa ikhwalithi ephezulu kakhulu.Eminyakeni edlule , sithole izimpendulo ezinhle kakhulu , hhayi nje ngoba siletha izixazululo ezinhle , kodwa nangenxa yesevisi yethu enhle ngemva kokuthengisa .Silapha silinde wena ukuze uthole uphenyo lwakho.