Abasebenzi bethu ngokuqeqeshwa okunolwazi.Ulwazi olunekhono lochwepheshe, umuzwa onamandla wokusiza, ukwanelisa izimfuno zesevisi zamakhasimende Zabahlinzeki Abaphezulu base-China I-Wholesale Nucleic Acid Extractor Automatic PCR Rna Extration Purification Natch CS2 Rna Extration and Purify, Ngoba sihlala nalo layini cishe iminyaka eyi-10.Sithole ukwesekwa kwabahlinzeki abaphumelela kakhulu kokuhle kakhulu nangezindleko.Futhi besinabahlinzeki bempahla abanekhwalithi ephezulu ephansi.Manje izimboni ezimbalwa ze-OEM zibambisane nathi futhi.
Abasebenzi bethu ngokuqeqeshwa okunolwazi.Ulwazi lochwepheshe abanamakhono, umuzwa onamandla wokusiza, ukwanelisa izidingo zesevisi zamakhasimendeInani eliphakeme lempahla ye-PCR, Umshini wePcr, Sinesipiliyoni seminyaka eminingi ekukhiqizeni umkhiqizo wezinwele, futhi Ithimba lethu le-QC eliqinile kanye nabasebenzi abanamakhono bazoqinisekisa ukuthi sikunikeza izinto zezinwele eziphezulu ezinekhwalithi engcono kakhulu yezinwele nomsebenzi.Uzothola ibhizinisi eliphumelelayo uma ukhetha ukubambisana nomkhiqizi onjalo ochwepheshe.Siyakwamukela ukubambisana kwe-oda lakho!

Incazelo

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Le khithi isebenzisa ikholomu yokuphotha kanye nefomula eyakhiwe yi-Foregene, engakhipha ngempumelelo i-RNA ephezulu nekhwalithi ephezulu kumaseli akhuliswe ngamapuleti emithombo engu-96, 24, 12, kanye ne-6.

Ikhithi inikeza Ikholomu Yokuhlanza I-DNA esebenza kahle, engahlukanisa kalula i-supernatant ne-cell lysate, ibophe futhi isuse i-genomic DNA.Ukusebenza kulula futhi konga isikhathi.

Ikholomu ye-RNA kuphela ingahlanganisa ngempumelelo i-RNA ngefomula ehlukile.Inani elikhulu lamasampula lingacutshungulwa kanyekanye.

Izingxenye zekhithi

*Sicela ugqoke amagilavu ​​futhi uthathe izinyathelo zokuvikela ngesikhathi sokusebenza njengoba i-Buffer cRL1 ne-Buffer RW1 iqukethe usawoti we-chaotropic ocasulayo.

Izici&izinzuzo

■ Yonke inqubo iqhutshwa ekamelweni lokushisa (15-25 ℃), ngaphandle kokugeza kweqhwa kanye nokushisa okuphansi kwe-centrifugation.
■ Ikhithi yonke ayinayo i-RNase-Free, asikho isidingo sokukhathazeka ngokuwohloka kwe-RNA.
■ Ikholomu Yokuhlanza I-DNA ibopha ngokukhethekile i-DNA, ukuze ikhithi ikwazi ukususa ukungcola kwe-DNA ye-genomic ngaphandle kokwengeza i-DNase eyengeziwe.
■ Isivuno esikhulu se-RNA: Ikholomu ye-RNA kuphela kanye nefomula eyingqayizivele ingahlanza i-RNA ngokuphumelelayo.
■ Isivinini esisheshayo: kulula ukusebenza futhi singaqedwa ngemizuzu eyi-11.
■ Ukuphepha: Asikho i-reagent ephilayo edingekayo.
■ Ikhwalithi ephezulu: I-RNA ehlanziwe imsulwa kakhulu, ayinamaprotheni nokunye ukungcola, futhi ingahlangabezana nokuhlola okuhlukahlukene okulandelayo.

Isicelo sekhithi

Ilungele ukukhishwa nokuhlanzwa kwe-RNA ephelele kumaseli akhulisiwe kumapuleti angama-96, 24, 12, kanye namapuleti angu-6.

Ukugeleza komsebenzi

Umdwebo

Umdwebo webhethri yejeli ye-agarose ye-Cell Total RNA Isolation Kit iphathe izinombolo ezingenhla ezihlukene zamaseli, ukukhishwa kwevolumu engu-20μl, thatha u-2μl isamba esihlanziwe se-RNA 1%.

Isitoreji nempilo yeshelufu

Ikhithi ingagcinwa izinyanga eziyi-12 ekamelweni lokushisa (15-25 ℃) noma 2-8 ℃ isikhathi eside (izinyanga ezingama-24).

I-Buffer cRL1 ingagcinwa ku-4 ℃ inyanga engu-1 ngemva kokwengeza i-2-hydroxy-1-ethanethiol(ongakukhetha).Abasebenzi bethu ngokuqeqeshwa abanolwazi.Ulwazi olunekhono lochwepheshe, umuzwa onamandla wokusiza, ukwanelisa izimfuno zesevisi zamakhasimende Zabahlinzeki Abaphezulu base-China I-Wholesale Nucleic Acid Extractor Automatic PCR Rna Extration Purification Natch CS2 Rna Extration and Purify, Ngoba sihlala nalo layini cishe iminyaka eyi-10.Sithole ukwesekwa kwabahlinzeki abaphumelela kakhulu kokuhle kakhulu nangezindleko.Futhi besinabahlinzeki bempahla abanekhwalithi ephezulu ephansi.Manje izimboni ezimbalwa ze-OEM zibambisane nathi futhi.
Abahlinzeki AbaphezuluInani eliphakeme lempahla ye-PCR, Umshini we-PCR, Sinesipiliyoni seminyaka eminingi ekukhiqizeni umkhiqizo wezinwele, futhi Ithimba lethu le-QC eliqinile kanye nabasebenzi abanekhono bazoqinisekisa ukuthi sikunikeza izinto zezinwele eziphezulu ezinekhwalithi engcono kakhulu yezinwele nomsebenzi.Uzothola ibhizinisi eliphumelelayo uma ukhetha ukubambisana nomkhiqizi onjalo ochwepheshe.Siyakwamukela ukubambisana kwe-oda lakho!