Enekhono eliphelele lesayensi lokuphatha elincomekayo, ikhwalithi enhle kakhulu nenkolo enhle kakhulu, sizuze igama elihle futhi sahlala kulo mkhakha Wezisetshenziswa Zelabhorethri Yomshini Wokuhlola we-PCR we-Professional China High Quality Eshibhile Isikhathi Sangempela Sesikhathi Sangempela se-PCR, Uma unentshisekelo kunoma yikuphi okuthengiswayo kwethu noma ufisa ukukhuluma ngokuthenga okufanelana nawe, kufanele uzizwe ukhululekile ngempela ukusithola.
Enekhono eliphelele lesayensi lokuphatha, ikhwalithi enhle kakhulu nenkolo enhle kakhulu, sithola igama elihle futhi sathatha lo mkhakhaUkuthola okuzenzakalelayo kwe-Nucleic Acid, I-China Rt PCR Detection Instruments, Inkampani yethu, ihlale iphathelene nekhwalithi njengesisekelo senkampani, ifuna intuthuko ngezinga eliphezulu lokwethembeka, ithobela izinga lokuphathwa kwekhwalithi ye-iso9000 ngokuqinile, idale inkampani eseqophelweni eliphezulu ngomoya wokwethembeka okumaka inqubekelaphambili nethemba.

Imininingwane

50 Preps, 200 Preps

Ikhithi isebenzisa ikholomu yokuphotha kanye nefomula eyakhiwe yi-Foregene, engakhipha ngempumelelo i-RNA ephezulu nekhwalithi ephezulu ezicutshini zezitshalo ezihlukahlukene ezinokuqukethwe okuphezulu kwama-polysaccharides noma ama-polyphenols.Ihlinzeka ngekholomu ye-DNA-Cleaning engasusa kalula i-genomic DNA ku-supernatant kanye ne-tissue lysate.Ikholomu ye-RNA kuphela ingabopha ngempumelelo i-RNA.Ikhithi ingacubungula inani elikhulu lamasampuli ngesikhathi esisodwa.

Lonke uhlelo aluqukethe i-RNase, ngakho-ke i-RNA ehlanziwe ngeke yehliswe.I-Buffer PRW1 kanye ne-Buffer PRW2 ingaqinisekisa ukuthi i-RNA etholiwe ayingcoliswanga amaprotheni, i-DNA, ama-ion, nezinhlanganisela eziphilayo.

Izingxenye zekhithi

Izici&izinzuzo

■ Ukusebenza ekamelweni lokushisa (15-25℃) kuyo yonke inqubo, ngaphandle kokugeza eqhweni nokushisa okuphansi kwe-centrifugation.
■ Ikhithi ephelele ye-RNase-Free, asikho isidingo sokukhathazeka ngokuwohloka kwe-RNA.
■ Ifaneleka ngokukhethekile ukuhlanzwa kwe-RNA kumasampula ezitshalo ama-polysaccharides nama-polyphenols.
■ Ikholomu Yokuhlanza I-DNA ibophezela ngokukhethekile ku-DNA, ukuze ikhithi isuse ukungcoliswa kwe-DNA ye-genomic ngaphandle kokwengeza i-DNase.
■ Isivuno esikhulu se-RNA: Ikholomu ye-RNA kuphela kanye nefomula eyingqayizivele ingahlanza i-RNA ngokuphumelelayo.
■ Isivinini esisheshayo: kulula ukusebenza futhi singaqedwa phakathi nemizuzu engama-30.
■ Ukuphepha: asikho i-reagent ephilayo edingekayo.
■ Ikhwalithi ephezulu: Izingcezwana ze-RNA ezihlanziwe zimsulwa kakhulu, azinawo amaphrotheni nokunye ukungcola, futhi zingahlangabezana nezinhlelo zokuhlola ezihlukahlukene ezansi nomfula.

Imingcele yomkhiqizo

■ Izinhlelo zokusebenza eziwela phansi: ukuhlanganiswa kwe-cDNA ye-first-strand, i-RT-PCR, i-molecular cloning, i-Northern Blot, njll.
■ Isampula: Izicubu zezitshalo ezintsha noma eziqandisiwe zama-polysaccharides nama-polyphenols
■ Umthamo: 50mg izicubu zezitshalo
■ Umthamo omkhulu wokubopha we-RNA wekholomu yokuhlanza: 80 μg
■ Ivolumu ye-Elution: 50-200 μl

Isicelo sekhithi

Ilungele ukukhishwa nokuhlanzwa kwe-RNA ephelele kumasampula ezicubu zezitshalo ezintsha noma eziqandisiwe (ikakhulukazi izicubu zamaqabunga ezitshalo ezintsha) ezinokuqukethwe okuphezulu kwe-polysaccharide kanye ne-polyphenol.

Ukugeleza komsebenzi

Umdwebo

I-Plant Total RNA Isolation Kit Plus icutshungulwe u-50mg wamaqabunga amasha e-polysaccharides nama-polyphenols, futhi i-RNA ehlanzekile engu-5% yahlolwa nge-electrophoresis.
1: Ubhanana
2: Ginkgo
3: Ukotini
4: Ihalanathi

Isitoreji kanye Nempilo yeshelufu

Le kit ingagcinwa izinyanga ezingu-24 ngaphansi kwezimo ezomile ekamelweni lokushisa (15-25℃);uma idinga ukugcinwa isikhathi eside, ingagcinwa ku-2–8℃.
I-Buffer PSL1 ingafakwa kokuthi 4℃ inyanga engu-1 ngemva kokwengeza i-β-mercaptoethanol (kuyanconywa ukuthi iyengeze ngesikhathi esifanayo sokuhlola).

Enekhono eliphelele lesayensi lokuphatha elincomekayo, ikhwalithi enhle kakhulu nenkolo enhle kakhulu, sizuze igama elihle futhi sahlala kulo mkhakha Wezisetshenziswa Zelabhorethri Yomshini Wokuhlola we-PCR we-Professional China High Quality Eshibhile Isikhathi Sangempela Sesikhathi Sangempela se-PCR, Uma unentshisekelo kunoma yikuphi okuthengiswayo kwethu noma ufisa ukukhuluma ngokuthenga okufanelana nawe, kufanele uzizwe ukhululekile ngempela ukusithola.
I-Professional ChinaI-China Rt PCR Detection Instruments, Ukuthola okuzenzakalelayo kwe-Nucleic Acid, Inkampani yethu, ihlale iphathelene nekhwalithi njengesisekelo senkampani, ifuna intuthuko ngezinga eliphezulu lokwethembeka, ithobela izinga lokuphathwa kwekhwalithi ye-iso9000 ngokuqinile, idale inkampani eseqophelweni eliphezulu ngomoya wokwethembeka okumaka inqubekelaphambili nethemba.