Ukuze kuhlangatshezwane nokwaneliseka okulindeleke kakhulu kwamakhasimende, sineqembu lethu eliqinile elizohlinzeka ngosekelo lwethu olungcono kakhulu kukho konke okuhlanganisa ukumaketha, imali engenayo, ukuvela, ukukhiqiza, ukuphatha okuhle kakhulu, ukupakisha, ukugcinwa kwempahla kanye nokusetshenziswa kwemikhiqizo Emisha Eshisayo Yase-China Plastic Magnetic Bead Method Universal Nucleic Acid Extraction Rapid Kit, futhi sibheke phambili kunoma iyiphi iklayenti, sibheke phambili kunoma iyiphi iklayenti.Qiniseka ukuthi uzizwa ungekho zindleko zokuxhumana nathi ukuze uthole ulwazi olwengeziwe.
Ukuze sihlangabezane nokwaneliseka okulindeleke kakhulu kwamakhasimende, sineqembu lethu eliqinile elizohlinzeka ngosekelo lwethu olungcono kakhulu kukho konke okuhlanganisa ukumaketha, imali engenayo, ukuvela, ukukhiqiza, ukuphatha okuhle kakhulu, ukupakisha, ukugcinwa kwempahla kanye nokusetshenziswa kwempahla.I-China Universal Nucleic Acid Extraction, Isizinda sePlastiki Nucleic Acid, Manje umncintiswano kulo mkhakha ushube kakhulu;kodwa sisazonikeza ikhwalithi engcono kakhulu, intengo enengqondo kanye nesevisi ecabangelayo emzamweni wokufinyelela umgomo wokunqoba.“Shintsha ube ngcono!”isiqubulo sethu, esisho ukuthi “Izwe elingcono liphambi kwethu, ngakho masilijabulele!”Shintsha ube ngcono!Usukulungele?

Imininingwane

50 Preps, 200 Preps

Ikhithi isebenzisa ikholomu yokuphotha kanye nefomula eyakhiwe yi-Foregene, engakhipha ngempumelelo i-RNA ephezulu nekhwalithi ephezulu ezicutshini zezitshalo ezihlukahlukene ezinokuqukethwe okuphansi kwama-polysaccharides nama-polyphenols.Kumasampula ezitshalo ane-polysaccharides ephezulu noma okuqukethwe kwama-polyphenols, kunconyelwa ukusebenzisa i-Plant Total RNA Isolation Plus Kit ukuze uthole imiphumela engcono yokukhipha i-RNA.Ikhithi inikeza ikholomu ye-DNA-Cleaning engasusa kalula i-genomic DNA ku-supernatant kanye ne-tissue lysate.Ikholomu ye-RNA kuphela ingabopha ngempumelelo i-RNA.Ikhithi ingacubungula inani elikhulu lamasampuli ngesikhathi esisodwa.

Lonke uhlelo aluqukethe i-RNase, ngakho-ke i-RNA ehlanziwe ngeke yehliswe.I-Buffer PRW1 kanye ne-Buffer PRW2 ingaqinisekisa ukuthi i-RNA etholiwe ayingcoliswanga amaprotheni, i-DNA, ama-ion, nezinhlanganisela eziphilayo.

Izingxenye zekhithi

Izici&izinzuzo

■ Ukusebenza ekamelweni lokushisa (15-25℃) kuyo yonke inqubo, ngaphandle kokugeza eqhweni nokushisa okuphansi kwe-centrifugation.
■ Ikhithi ephelele ye-RNase-Free, asikho isidingo sokukhathazeka ngokuwohloka kwe-RNA.
■ Ikholomu Yokuhlanza I-DNA ibophezela ngokukhethekile ku-DNA, ukuze ikhithi isuse ukungcoliswa kwe-DNA ye-genomic ngaphandle kokwengeza i-DNase.
■ Isivuno esikhulu se-RNA: Ikholomu ye-RNA kuphela kanye nefomula eyingqayizivele ingahlanza i-RNA ngokuphumelelayo.
■ Isivinini esisheshayo: kulula ukusebenza futhi singaqedwa phakathi nemizuzu engama-30.
■ Ukuphepha: asikho i-reagent ephilayo edingekayo.
■ Ikhwalithi ephezulu: Izingcezwana ze-RNA ezihlanziwe zimsulwa kakhulu, azinawo amaphrotheni nokunye ukungcola, futhi zingahlangabezana nezinhlelo zokuhlola ezihlukahlukene ezansi nomfula.

Isicelo sekhithi

Ilungele ukukhishwa nokuhlanzwa kwe-RNA ephelele kumasampula ezicubu zezitshalo ezintsha noma eziqandisiwe (ikakhulukazi izicubu zamaqabunga ezitshalo ezintsha) ezine-polysaccharide ephansi kanye nokuqukethwe kwe-polyphenol.

Ukugeleza komsebenzi

Umdwebo

I-Plant Total RNA Isolation Kit Plus icutshungulwe u-50mg wamaqabunga amasha e-polysaccharides nama-polyphenols, futhi i-RNA ehlanzekile engu-5% yahlolwa nge-electrophoresis.
1: Ubhanana
2: Ginkgo
3: Ukotini
4: Ihalanathi

Isitoreji nempilo yeshelufu

Ikhithi ingagcinwa izinyanga eziyi-12 ekamelweni lokushisa (15-25 ℃) endaweni eyomile, kanye no-2-8 ℃ isikhathi eside (izinyanga ezingu-24).

I-Buffer PSL1 ingagcinwa ku-4 ℃ inyanga engu-1 ngemva kokwengeza i-2-hydroxy-1-ethanethiol(ongakukhetha). Ukuze sihlangabezane nokwaneliseka obekulindeleke kakhulu kumakhasimende , sineqembu lethu eliqinile elizohlinzeka ngosekelo lwethu olungcono kakhulu kukho konke okuhlanganisa ukumaketha, imali engenayo, ukuvela, ukukhiqiza, ukuphatha okuhle kakhulu, ukupakisha, i-warehousing yePlastiki ye-Acthousing ye-Acthousing ye-Plastiki ye-Magthousing ye-China kanye ne-Logistics ye-China yonke I-Extraction Rapid Kit, Okwamanje, sibheke phambili ekusebenzisaneni okukhulu nakakhulu namakhasimende aphesheya okusekelwe ezinzuzweni ezifanayo.Qiniseka ukuthi uzizwa ungekho zindleko zokuxhumana nathi ukuze uthole ulwazi olwengeziwe.
Imikhiqizo Emisha EshisayoI-China Universal Nucleic Acid Extraction, Isizinda sePlastiki Nucleic Acid, Manje umncintiswano kulo mkhakha ushube kakhulu;kodwa sisazonikeza ikhwalithi engcono kakhulu, intengo enengqondo kanye nesevisi ecabangelayo emzamweni wokufinyelela umgomo wokunqoba.“Shintsha ube ngcono!”isiqubulo sethu, esisho ukuthi “Izwe elingcono liphambi kwethu, ngakho masilijabulele!”Shintsha ube ngcono!Usukulungele?