Isampula yamahhala ye-China High Sensitivity DNA/Rna One-step Probe Rt-Qpcr Kit V2
Incazelo yekhithi:
◮Ikhithi yesinyathelo esisodwa yenza ukuloba okuhlanekezelwe kanye ne-qPCR ukusabela okubili kushubhu efanayo, idinga kuphela ukwengeza ithempulethi i-RNA, iziqalo ezithile ze-PCR kanye ne-RNase-Free ddH.2O.
◮Ikhithi ingahlaziya ngokushesha nangempumelelo i-RNA yegciwane noma ilandele i-RNA.
◮Ikhithi isebenzisa i-Foregene reverse transcription reagent eyingqayizivele kanye ne-Foregene HotStar Taq DNA Polymerase ehlanganiswe nesistimu yokusabela ehlukile ukuze kuthuthukiswe ngempumelelo ukusebenza kahle kokukhulisa nokucaciswa kokusabela.
◮Isistimu yokusabela ethuthukisiwe yenza ukusabela kube nokuzwela kokutholwa okuphezulu, ukuzinza okuqinile kokushisa, nokubekezelelana okungcono.
◮I-RT-qPCR EasyTM(One Step) -SYBR Green I kit iza nodayi wereferensi wangaphakathi we-ROX, ongasetshenziswa ukuqeda ingemuva lesignali namaphutha esignali phakathi kwemithombo, okulula ukuthi amakhasimende ayisebenzise kumamodeli ahlukene wamathuluzi e-PCR amaningi.
Sikholelwa kulokhu: Ukuqamba okusha kungumphefumulo nomoya wethu.Izinga eliphezulu liyimpilo yethu.Umthengi okudingeka abe nakho nguNkulunkulu wethu wesampula Yamahhala ye-China High Sensitivity DNA/Rna One-step Probe Rt-QpcrKit V2, Ngaphakathi kwezinhlelo zethu, sesivele sinezitolo eziningi eShayina futhi izixazululo zethu zizuze ukunconywa kubathengi emhlabeni wonke.Yamukela abathengi abasha nabaphelelwe yisikhathi ukuze uxhumane nathi ebudlelwaneni bebhizinisi obuhlala isikhathi eside.
Sikholelwa kulokhu: Ukuqamba okusha kungumphefumulo nomoya wethu.Izinga eliphezulu liyimpilo yethu.Umthengi okudingeka abe nakho nguNkulunkulu wethuI-China Taq DNA Polymerase, Qpcr, Ukusebenza kanzima ukuze uqhubeke nokwenza inqubekelaphambili, ukusungula izinto ezintsha embonini, yenza yonke imizamo ebhizinisini elisezingeni lokuqala.Sizama konke okusemandleni ethu ukwakha imodeli yokuphatha yesayensi, ukufunda ulwazi oluningi lochwepheshe, ukuthuthukisa imishini yokukhiqiza ethuthukisiwe nenqubo yokukhiqiza , ukwakha imikhiqizo yekhwalithi yokuqala, intengo enengqondo, izinga eliphezulu lesevisi , ukulethwa okusheshayo , ukukunikeza ukudala inani elisha .
Imininingwane
50×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns
Izingxenye zekhithi
| Ingxenye I | Isilondolozi se-P1 |
| Isilondolozi se-P2 | |
| I-6×I-DNA Yokulayisha Ibhafa | |
| Ingxenye II | I-2 × I-PCR yeLeaf EasyTMImiksi(UNG) |
Izici&izinzuzo
■ Akukho ukuhlanzwa kwe-DNA okuchitha isikhathi nokubizayo
■ Impahla encane
■ Kulula-isampula ingaba ngaphansi kokusabela kwe-PCR noma ukusabela kwe-lysis ngaphandle kokusika noma ukugaya
■ 2 × Mix, ukunciphisa amaphutha okulayisha amasampula kanye nesikhathi sokulungiselela uhlelo lokusabela
■ Ukulungiselela isifanekiso esisheshayo kungaqedwa ngemizuzu eyi-10, ukusabela kwe-PCR kungaqedwa okungenani ngemizuzu engama-50
■ Ukusabela okuphezulu kwe-throughput-lysis kungaqedwa ngepuleti le-PCR le-96-well
Imingcele yekhithi
Isicelo: ukuhlonza okushintshwe izakhi, i-genotyping, njll.
Isampula: iqabunga lesitshalo
Isikali: Ububanzi 2-3mm (indlela eqondile), ububanzi 5-7mm (indlela ye-lysis)
Ibanga lokutholwa: isiqeshana esiqondiwe ≤1kb
Ukugeleza komsebenzi
Electropherogram
Amaviki amabili emiphumela yokutholwa kwe-PCR Mix ngaphansi kwesimo sokungcoliswa komkhiqizo wokukhulisa i-PCR.Sikholelwa kokuthi: Ukuqamba okusha kuwumphefumulo nomoya wethu.Izinga eliphezulu liyimpilo yethu.Umthengi okudingeka abe nawo uNkulunkulu wethu wesampula Yamahhala ye-China High Sensitivity DNA/Rna One-Step Probe Rt-Qpcr Kit V2, Ngaphakathi kwezinhlelo zethu, sesivele sinezitolo eziningi e-China futhi izixazululo zethu sezizuze ukunconywa kubathengi emhlabeni wonke.Yamukela abathengi abasha nabaphelelwe yisikhathi ukuze uxhumane nathi ebudlelwaneni bebhizinisi obuhlala isikhathi eside.
Isampula yamahhala yeI-China Taq DNA Polymerase, Qpcr, Ukusebenza kanzima ukuze uqhubeke wenza inqubekelaphambili, ukuqamba okusha embonini, yenza yonke imizamo ebhizinisini elisezingeni lokuqala.Sizama konke okusemandleni ethu ukwakha imodeli yokuphatha yesayensi, ukufunda ulwazi oluningi lochwepheshe, ukuthuthukisa imishini yokukhiqiza ethuthukisiwe nenqubo yokukhiqiza , ukwakha imikhiqizo yekhwalithi yokuqala, intengo enengqondo, izinga eliphezulu lesevisi , ukulethwa okusheshayo , ukukunikeza ukudala inani elisha .
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