Ukuqamba izinto ezintsha, ikhwalithi enhle kanye nokwethembeka kungamagugu asemqoka ebhizinisi lethu.Lezi zimiso namuhla ngaphezu kwanini ngaphambili zakha isisekelo sempumelelo yethu njengenhlangano esebenza emhlabeni wonke emaphakathi yekhwalithi Enhle Yekhwalithi Ephakeme Isikhathi Sangempela I-PCR Kit Hotstart Taq DNA Polymerase Power Green Qpcr Mix, Inkampani yethu izinikele ekuhlinzekeni amakhasimende ngemikhiqizo yekhwalithi ephezulu nezinzile ngentengo yokuncintisana, okwenza wonke amakhasimende aneliseke ngemikhiqizo namasevisi ethu.
Ukuqamba izinto ezintsha, ikhwalithi enhle kanye nokwethembeka kungamagugu asemqoka ebhizinisi lethu.Le migomo namuhla eyengeziwe kunangaphambili yenza isisekelo sempumelelo yethu njengenhlangano yamazwe omhlaba ephakathi nendawoI-China Taq DNA Polymerase ne-Qpcr, Uma uthanda noma yiziphi izinto zethu ezikulandelayo lapho ubuka uhlu lwemikhiqizo yethu, sicela ukhululeke ukuxhumana nathi ukuze uthole imibuzo.Uzokwazi ukusithumelela ama-imeyili futhi uxhumane nathi ukuze kuboniswane futhi sizokuphendula ngokushesha lapho sesikwazi ukukwenza.Uma kufaneleka, ungathola ikheli lethu kusizindalwazi sethu futhi uze ebhizinisini lethu.noma ulwazi olwengeziwe lwezinto zethu uwedwa.Ngokuvamile sikulungele ukwakha ubudlelwano bokubambisana obude nobuzinzile nanoma ibaphi abathengi abangaba khona emikhakheni ehlobene.

Izincazelo zekhithi

I-2×Real PCR Easy^TMI-Mix-SYBR ihlinzekwa yi-Real Time PCR Easy^TM-Ikhithi ye-SYBR Green I iwuhlelo olusha lwe-premix olusebenzisa i-SYBR Green I ye-Real Time PCR ukusabela kokukhulisa, okungathuthukisa kakhulu ukucaciswa komkhiqizo nokuzwela kokusabela .I-ROX inikezwa njengodayi wokulawula wangaphakathi.Amandla e-fluorescence ale khithi aphindwe izikhathi ezingu-3-5 kunemikhiqizo efanayo, engazwela kakhulu futhi ibonise ngokunembile ukugxila kwesifanekiso se-DNA esihlosiwe.

2×I-PCR Yangempela Kulula^TMI-Mix-SYBR iqukethe i-Taq DNA Polymerase eyingqayizivele ye-Foregene.Uma iqhathaniswa nama-enzyme e-Taq ajwayelekile, inezinzuzo zokusebenza kahle kokukhulisa i-amplification, ikhono elithile eliqinile lokukhulisa kanye nezinga lokungafani eliphansi.Inganciphisa ukukhulisa okungaqondile futhi ithuthukise ukunemba kwe-PCR.

Imininingwane

Izici&izinzuzo

■ I-Simple—2X PCR Mix ukuze kwehliswe iphutha lokuhlola nesikhathi sokusebenza

■ Ibhafa ecacisiwe—elungiselelwe kahle kanye ne-enzyme ye-Taq eqala ukushisa ingavimbela ukukhulisa okungaqondile kanye nokwakheka kwe-primer dimer.

■ Ukuzwela okuphezulu—kukwazi ukubona amakhophi aphansi esifanekiso

■ Ukuguquguquka okuhle—kuhambisana nezinsimbi eziningi zesikhathi sangempela ze-PCR

Isicelo sekhithi

Ukuhlaziywa kwe-qPCR

Ukugeleza komsebenzi

Umfanekiso

Isitoreji nempilo yeshelufu

Le kit kufanele igcinwe kude nokukhanya futhi kufanele igcinwe ku -20 ℃.Uma isetshenziswa njalo, ingabuye igcinwe ku-4℃ isikhathi esifushane (izinsuku eziyi-10).

Ukuqamba izinto ezintsha, ikhwalithi enhle kanye nokwethembeka kungamagugu asemqoka ebhizinisi lethu.Lezi zimiso namuhla ezingaphezu kunangaphambili zakha isisekelo sempumelelo yethu njengenhlangano esebenza emhlabeni wonke yosayizi omaphakathi yekhwalithi Enhle Yekhwalithi Ephakeme Isikhathi Sangempela I-PCR Kit Hotstart Taq DNA Polymerase Power Green Qpcr Mix , Inkampani yethu izinikele ekuhlinzekeni amakhasimende ngemikhiqizo yekhwalithi ephezulu nezinzile ngentengo yokuncintisana, okwenza wonke amakhasimende aneliseke ngemikhiqizo namasevisi ethu.
Ikhwalithi enhleI-China Taq DNA Polymerase ne-Qpcr, Uma uthanda noma yiziphi izinto zethu ezikulandelayo lapho ubuka uhlu lwemikhiqizo yethu, sicela ukhululeke ukuxhumana nathi ukuze uthole imibuzo.Uzokwazi ukusithumelela ama-imeyili futhi uxhumane nathi ukuze kuboniswane futhi sizokuphendula ngokushesha lapho sesikwazi ukukwenza.Uma kufaneleka, ungathola ikheli lethu kusizindalwazi sethu futhi uze ebhizinisini lethu.noma ulwazi olwengeziwe lwezinto zethu uwedwa.Ngokuvamile sikulungele ukwakha ubudlelwano bokubambisana obude nobuzinzile nanoma ibaphi abathengi abangaba khona emikhakheni ehlobene.