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Ifekthri ehlinzekwe ngefekthri Umshini Wokukhipha I-Nucleic Acid Umshini Ozenzakalelayo Wezisetshenziswa Zezokwelapha I-Nucleic Acid Isistimu Yokukhipha I-Rna/I-DNA Extraction Instrument

Incazelo yekhithi:

 

I-Cat.No.DR-01011/01012/01013

 

Ukuze kuhlanzwe i-viral DNA/RNA ku-plasma, i-serum, uketshezi lomzimba olungenamaseli, amandla amakhulu e-cell-culture.

Khipha ngokushesha futhi uhlanze i-DNA yegciwane noma i-RNA kumasampula afana ne-plasma, i-serum, uketshezi lomzimba olungenamaseli kanye ne-cell culture supernatant.

Akukho ukucekelwa phansi kwe-RNA.Yonke ikhithi i-RNase-Free

Kulula—yonke imisebenzi iqedwa ezingeni lokushisa legumbi

Ukusheshisa—ukusebenza kungaqedwa ngemizuzu engu-20

Isivuno esikhulu se-RNA: Ikholomu ye-RNA kuphela kanye nefomula eyingqayizivele ingahlanza i-RNA ngokuphumelelayo

Iphephile—ayikho i-organic reagent esetshenzisiwe

Umthamo omkhulu wokucubungula isampula—amasampula angafika ku-200μl angacutshungulwa isikhathi ngasinye.


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  • Imininingwane Yomkhiqizo

    Omaka bomkhiqizo

    FAQ

    LANDA IZIsetshenziswa

    Ikhomishini yethu ukuhlinzeka abasebenzisi bethu bokugcina kanye namakhasimende ngempahla yedijithali esezingeni eliphezulu kakhulu nencintisanayo yeFactory ehlinzekwe yi-Nucleic Acid Extraction Machine Automated Medical Equipment Nucleic Acid Extraction System Rna/DNA Extraction Instrument, Amakhasimende ethu asatshalaliswa ikakhulukazi eNyakatho Melika, e-Afrika naseMpumalanga Yurophu.siyakwazi ukuhlinzeka ngezimpahla zekhwalithi ye-premium ngamathegi wamanani anolaka ngendlela emangalisayo.
    Umsebenzi wethu ukuhlinzeka abasebenzisi bethu bokugcina kanye namakhasimende ngekhwalithi ephezulu kakhulu kanye nokuncintisana kwezimpahla zedijithali eziphathwayoUmshini Wokukhipha waseChina kanye nokuhlolwa kwe-Nucleic Acid, Manje sesithumele izimpahla zethu emhlabeni wonke, ikakhulukazi i-USA namazwe aseYurophu.Ngaphezu kwalokho, zonke izinto zethu zakhiwe ngemishini ethuthukisiwe kanye nezinqubo eziqinile ze-QC zokuqinisekisa ikhwalithi ephezulu.Uma unentshisekelo kunoma yikuphi okuthengiswayo kwethu, khumbula ungangabazi ukusithinta.Sizozama konke okusemandleni ethu ukuhlangabezana nezidingo zakho.
    Amamanuwali wemiyalo:

    Ikhomishini yethu ukuhlinzeka abasebenzisi bethu bokugcina kanye namakhasimende ngempahla yedijithali esezingeni eliphezulu kakhulu nencintisanayo yeFactory ehlinzekwe yi-Nucleic Acid Extraction Machine Automated Medical Equipment Nucleic Acid Extraction System Rna/DNA Extraction Instrument, Amakhasimende ethu asatshalaliswa ikakhulukazi eNyakatho Melika, e-Afrika naseMpumalanga Yurophu.siyakwazi ukuhlinzeka ngezimpahla zekhwalithi ye-premium ngamathegi wamanani anolaka ngendlela emangalisayo.
    Ifekthri inikeziweUmshini Wokukhipha waseChina kanye nokuhlolwa kwe-Nucleic Acid, Manje sesithumele izimpahla zethu emhlabeni wonke, ikakhulukazi i-USA namazwe aseYurophu.Ngaphezu kwalokho, zonke izinto zethu zakhiwe ngemishini ethuthukisiwe kanye nezinqubo eziqinile ze-QC zokuqinisekisa ikhwalithi ephezulu.Uma unentshisekelo kunoma yikuphi okuthengiswayo kwethu, khumbula ungangabazi ukusithinta.Sizozama konke okusemandleni ethu ukuhlangabezana nezidingo zakho.


  • Okwedlule:
  • Olandelayo:

  • Umhlahlandlela Wokuhlaziya Inkinga

    Okulandelayo ukuhlaziya izinkinga ezingase kuhlangatshezwane nazo ekukhishweni kwe-viral DNA/RNA, ngethemba lokuba usizo ekuhloleni kwakho.Ngaphezu kwalokho, kwezinye izinkinga zokuhlola noma zobuchwepheshe ngaphandle kwemiyalo yokusebenza nokuhlaziya inkinga, sinikele ngosekelo lobuchwepheshe ukuze sikusize.Uma unezinye izidingo, sicela usithinte: 028-83360257 noma E-mail:

    Tech@foregene.com.

      

    Akukho ukukhishwa kwe-nucleic acid noma isivuno esiphansi se-nucleic acid

    Ngokuvamile kuba nezinto eziningi ezithinta ukusebenza kahle kokululama, njengalezi: isampula yokuqukethwe kwe-nucleic acid, indlela yokusebenza, ivolumu ye-elution, njll.

    Ukuhlaziywa kwezimbangela ezijwayelekile:

    1. Ukugeza kweqhwa noma izinga lokushisa eliphansi (4 ° C) i-centrifugation yenziwa ngesikhathi senqubo.

    Ukusikisela: Sebenzisa ekamelweni lokushisa (15-25°C) kuyo yonke inqubo, ungabhavi eqhweni kanye nokushisa okuphansi kwe-centrifugation.

    2. Isampula ligcinwe ngendlela engafanele noma isampula ligcinwe isikhathi eside kakhulu.

    Okutuswayo: Gcina amasampula ku -80°C futhi ugweme ukuqhwa okuphindaphindiwe nokuncibilika;zama ukusebenzisa amasampula asanda kuqoqwa ukuze kukhishwe i-nucleic acid.

    3. Isampula yesampula enganele.

    Okutuswayo: Sicela uqinisekise ukuthi isampula kanye nesisombululo sokusebenza se-lysis kuxutshwe kahle futhi kufakwe endaweni yokushisa yegumbi (15-25°C) imizuzu eyi-10.

    4. Ukwengezwa okungalungile kwe-eluent.

    Isiphakamiso: Qiniseka ukuthi i-ddH2O Engenayo i-RNase yengezwa ngokudonsela phansi maphakathi nolwelwesi lwekholomu yokuhlanza, futhi ungayibeki eringini yekholomu yokuhlanza.

    5. Ivolumu elungile ye-ethanol ephelele ayizange yengezwe ku-Buffer RW2.

    Ukusikisela: Sicela ulandele imiyalelo, wengeze ivolumu efanele ye-ethanol ephelele ku-Buffer RW2 futhi uhlanganise kahle ngaphambi kokusebenzisa ikhithi.

    6. Ivolumu yesampula engafanele.

    Isiphakamiso: U-200µl wesampula ucutshungulwa kuwo wonke u-500µl we-Buffer DRL.Ukucutshungulwa kwesampula okweqile kuzoholela emvuzweni ephansi yokukhipha i-nucleic acid.

    7. Ivolumu ye-elution engafanele noma ukungezwani okuphelele.

    Isincomo: Ivolumu ecacile yekholomu yokuhlanza ingu-30-50μl;uma umphumela we-elution ungagculisi, kuyanconywa ukuthi unwebe isikhathi kuzinga lokushisa legumbi ngemva kokwengeza i-ddH2O eshisiwe ye-RNase-Free, njenge-5-10min.

    8. I-Ethanol ihlala kukholamu ngemva kokugeza nge-Buffer RW2.

    Isiphakamiso: Uma i-ethanol isala ngemva kokufakwa phakathi kwe-centrifugation ne-Buffer RW2 imizuzu emi-2, ikholomu ingabekwa ekamelweni lokushisa imizuzu emi-5 ngemva kwe-centrifugation ukuze ikhiphe ngokuphelele i-ethanol esele.

     

    I-nucleic acid ehlanzekile iyancipha

    Izinga le-nucleic acid ehlanzekile lihlobene nokulondolozwa kwesampula, ukungcoliswa kwe-RNase, ukusebenza nezinye izici.Ukuhlaziywa kwezimbangela ezijwayelekile:

    1. Amasampula aqoqiwe awagcinwanga ngesikhathi.

    Ukusikisela: Uma isampuli ingasetshenziswa ngesikhathi ngemva kokuqoqwa, sicela uyigcine ku -80°C ezingeni lokushisa eliphansi ngokushesha.Ukuze uthole i-RNA, zama ukusebenzisa amasampuli asanda kuqoqwa.

    2. Qoqa amasampula bese uwaqandisa futhi uncibilike ngokuphindaphindiwe.

    Ukusikisela: Gwema ukuqanda nokuncibilika (hhayi ngaphezu kokukodwa) ngesikhathi sokuqoqwa nokugcinwa kwamasampula, ngaphandle kwalokho isivuno se-nucleic acid sizoncipha.

    3. I-RNase yethulwa egumbini lokuhlinza noma amagilavu ​​alahlwayo, imaski, njll. awagqokwa.

    Okutuswayo: Ukuhlolwa kokukhipha i-RNA kwenziwa kangcono kakhulu ekamelweni lokusebenza elihlukile le-RNA, futhi itafula laselabhorethri kufanele lihlanzwe ngaphambi kokuhlolwa.

    Gqoka amagilavu ​​alahlwayo namamaski ngesikhathi sokuhlolwa ukuze ugweme ukucekelwa phansi kwe-RNA okubangelwa ukwethulwa kwe-RNase ngezinga elikhulu kakhulu.

    4. I-reagent yonakaliswe i-RNase ngesikhathi sokusetshenziswa.

    Isincomo: Faka esikhundleni se-Viral DNA/RNA Isolation Kit entsha ukuze uthole ukuhlolwa okuhlobene.

    5. Amashubhu e-centrifuge namathiphu e-piette asetshenziselwa ukukhwabanisa kwe-RNA angcoliswe yi-RNase.

    Ukusikisela: Qinisekisa ukuthi amashubhu e-centrifuge, amathiphu e-pipette, amapayipi, njll. asetshenziselwa ukukhipha i-RNA konke Akuna-RNAse.

     

    I-nucleic acid ehlanjululwe ithinta ukuhlolwa okungezansi komfula

    I-DNA ne-RNA ehlanjululwe yikholomu yokuhlanza, uma i-ion kasawoti kanye nokuqukethwe kwamaprotheni kuphezulu kakhulu, kuzothinta ukuhlolwa okuya phansi komfula, okufana nalokhu: Ukukhulisa i-PCR, ukuloba okuhlanekezelwe, njll.

    1. I-DNA ekhishiwe kanye ne-RNA inama-ion kasawoti asele.

    Isiphakamiso: Qinisekisa ukuthi ivolumu elungile ye-ethanol ephelele yengezwa ku-Buffer RW2, futhi ugeze ikholomu yokuhlanza kabili ngesivinini sokuhlanganisa esishiwo emiyalweni yokusebenza;Yenza i-centrifugation ukuze unciphise ukungcoliswa kwe-ion kasawoti.

    2. I-DNA ekhishiwe kanye ne-RNA inezinsalela ze-ethanol.

    Ukusikisela: Ngemva kokuqinisekisa ukugeza nge-Buffer RW2, yenza i-tube centrifugation engenalutho ngesivinini se-centrifugation emiyalweni yokusebenza;uma kusekhona izinsalela ze-ethanol, ungakwazi ukufaka i-centrifuge ishubhu elingenalutho bese ulibeka endaweni yokushisa yasekamelweni imizuzu engu-5 ukuze ususe insalela ye-ethanol ngezinga elikhulu kakhulu.

    Amamanuwali wemiyalo:

    I-Viral DNA&RNA Isolation Kit Instruction Manual

     

    Bhala umyalezo wakho lapha futhi usithumelele wona