Ifekthri ehlinzekwe ngefekthri Umshini Wokukhipha I-Nucleic Acid Umshini Ozenzakalelayo Wezisetshenziswa Zezokwelapha I-Nucleic Acid Isistimu Yokukhipha I-Rna/I-DNA Extraction Instrument
Ikhomishini yethu ukuhlinzeka abasebenzisi bethu bokugcina kanye namakhasimende ngempahla yedijithali esezingeni eliphezulu kakhulu nencintisanayo yeFactory ehlinzekwe yi-Nucleic Acid Extraction Machine Automated Medical Equipment Nucleic Acid Extraction System Rna/DNA Extraction Instrument, Amakhasimende ethu asatshalaliswa ikakhulukazi eNyakatho Melika, e-Afrika naseMpumalanga Yurophu.siyakwazi ukuhlinzeka ngezimpahla zekhwalithi ye-premium ngamathegi wamanani anolaka ngendlela emangalisayo.
Umsebenzi wethu ukuhlinzeka abasebenzisi bethu bokugcina kanye namakhasimende ngekhwalithi ephezulu kakhulu kanye nokuncintisana kwezimpahla zedijithali eziphathwayoUmshini Wokukhipha waseChina kanye nokuhlolwa kwe-Nucleic Acid, Manje sesithumele izimpahla zethu emhlabeni wonke, ikakhulukazi i-USA namazwe aseYurophu.Ngaphezu kwalokho, zonke izinto zethu zakhiwe ngemishini ethuthukisiwe kanye nezinqubo eziqinile ze-QC zokuqinisekisa ikhwalithi ephezulu.Uma unentshisekelo kunoma yikuphi okuthengiswayo kwethu, khumbula ungangabazi ukusithinta.Sizozama konke okusemandleni ethu ukuhlangabezana nezidingo zakho.
Amamanuwali wemiyalo:
Ikhomishini yethu ukuhlinzeka abasebenzisi bethu bokugcina kanye namakhasimende ngempahla yedijithali esezingeni eliphezulu kakhulu nencintisanayo yeFactory ehlinzekwe yi-Nucleic Acid Extraction Machine Automated Medical Equipment Nucleic Acid Extraction System Rna/DNA Extraction Instrument, Amakhasimende ethu asatshalaliswa ikakhulukazi eNyakatho Melika, e-Afrika naseMpumalanga Yurophu.siyakwazi ukuhlinzeka ngezimpahla zekhwalithi ye-premium ngamathegi wamanani anolaka ngendlela emangalisayo.
Ifekthri inikeziweUmshini Wokukhipha waseChina kanye nokuhlolwa kwe-Nucleic Acid, Manje sesithumele izimpahla zethu emhlabeni wonke, ikakhulukazi i-USA namazwe aseYurophu.Ngaphezu kwalokho, zonke izinto zethu zakhiwe ngemishini ethuthukisiwe kanye nezinqubo eziqinile ze-QC zokuqinisekisa ikhwalithi ephezulu.Uma unentshisekelo kunoma yikuphi okuthengiswayo kwethu, khumbula ungangabazi ukusithinta.Sizozama konke okusemandleni ethu ukuhlangabezana nezidingo zakho.
Umhlahlandlela Wokuhlaziya Inkinga
Okulandelayo ukuhlaziya izinkinga ezingase kuhlangatshezwane nazo ekukhishweni kwe-viral DNA/RNA, ngethemba lokuba usizo ekuhloleni kwakho.Ngaphezu kwalokho, kwezinye izinkinga zokuhlola noma zobuchwepheshe ngaphandle kwemiyalo yokusebenza nokuhlaziya inkinga, sinikele ngosekelo lobuchwepheshe ukuze sikusize.Uma unezinye izidingo, sicela usithinte: 028-83360257 noma E-mail:
Tech@foregene.com.
Akukho ukukhishwa kwe-nucleic acid noma isivuno esiphansi se-nucleic acid
Ngokuvamile kuba nezinto eziningi ezithinta ukusebenza kahle kokululama, njengalezi: isampula yokuqukethwe kwe-nucleic acid, indlela yokusebenza, ivolumu ye-elution, njll.
Ukuhlaziywa kwezimbangela ezijwayelekile:
1. Ukugeza kweqhwa noma izinga lokushisa eliphansi (4 ° C) i-centrifugation yenziwa ngesikhathi senqubo.
Ukusikisela: Sebenzisa ekamelweni lokushisa (15-25°C) kuyo yonke inqubo, ungabhavi eqhweni kanye nokushisa okuphansi kwe-centrifugation.
2. Isampula ligcinwe ngendlela engafanele noma isampula ligcinwe isikhathi eside kakhulu.
Okutuswayo: Gcina amasampula ku -80°C futhi ugweme ukuqhwa okuphindaphindiwe nokuncibilika;zama ukusebenzisa amasampula asanda kuqoqwa ukuze kukhishwe i-nucleic acid.
3. Isampula yesampula enganele.
Okutuswayo: Sicela uqinisekise ukuthi isampula kanye nesisombululo sokusebenza se-lysis kuxutshwe kahle futhi kufakwe endaweni yokushisa yegumbi (15-25°C) imizuzu eyi-10.
4. Ukwengezwa okungalungile kwe-eluent.
Isiphakamiso: Qiniseka ukuthi i-ddH2O Engenayo i-RNase yengezwa ngokudonsela phansi maphakathi nolwelwesi lwekholomu yokuhlanza, futhi ungayibeki eringini yekholomu yokuhlanza.
5. Ivolumu elungile ye-ethanol ephelele ayizange yengezwe ku-Buffer RW2.
Ukusikisela: Sicela ulandele imiyalelo, wengeze ivolumu efanele ye-ethanol ephelele ku-Buffer RW2 futhi uhlanganise kahle ngaphambi kokusebenzisa ikhithi.
6. Ivolumu yesampula engafanele.
Isiphakamiso: U-200µl wesampula ucutshungulwa kuwo wonke u-500µl we-Buffer DRL.Ukucutshungulwa kwesampula okweqile kuzoholela emvuzweni ephansi yokukhipha i-nucleic acid.
7. Ivolumu ye-elution engafanele noma ukungezwani okuphelele.
Isincomo: Ivolumu ecacile yekholomu yokuhlanza ingu-30-50μl;uma umphumela we-elution ungagculisi, kuyanconywa ukuthi unwebe isikhathi kuzinga lokushisa legumbi ngemva kokwengeza i-ddH2O eshisiwe ye-RNase-Free, njenge-5-10min.
8. I-Ethanol ihlala kukholamu ngemva kokugeza nge-Buffer RW2.
Isiphakamiso: Uma i-ethanol isala ngemva kokufakwa phakathi kwe-centrifugation ne-Buffer RW2 imizuzu emi-2, ikholomu ingabekwa ekamelweni lokushisa imizuzu emi-5 ngemva kwe-centrifugation ukuze ikhiphe ngokuphelele i-ethanol esele.
I-nucleic acid ehlanzekile iyancipha
Izinga le-nucleic acid ehlanzekile lihlobene nokulondolozwa kwesampula, ukungcoliswa kwe-RNase, ukusebenza nezinye izici.Ukuhlaziywa kwezimbangela ezijwayelekile:
1. Amasampula aqoqiwe awagcinwanga ngesikhathi.
Ukusikisela: Uma isampuli ingasetshenziswa ngesikhathi ngemva kokuqoqwa, sicela uyigcine ku -80°C ezingeni lokushisa eliphansi ngokushesha.Ukuze uthole i-RNA, zama ukusebenzisa amasampuli asanda kuqoqwa.
2. Qoqa amasampula bese uwaqandisa futhi uncibilike ngokuphindaphindiwe.
Ukusikisela: Gwema ukuqanda nokuncibilika (hhayi ngaphezu kokukodwa) ngesikhathi sokuqoqwa nokugcinwa kwamasampula, ngaphandle kwalokho isivuno se-nucleic acid sizoncipha.
3. I-RNase yethulwa egumbini lokuhlinza noma amagilavu alahlwayo, imaski, njll. awagqokwa.
Okutuswayo: Ukuhlolwa kokukhipha i-RNA kwenziwa kangcono kakhulu ekamelweni lokusebenza elihlukile le-RNA, futhi itafula laselabhorethri kufanele lihlanzwe ngaphambi kokuhlolwa.
Gqoka amagilavu alahlwayo namamaski ngesikhathi sokuhlolwa ukuze ugweme ukucekelwa phansi kwe-RNA okubangelwa ukwethulwa kwe-RNase ngezinga elikhulu kakhulu.
4. I-reagent yonakaliswe i-RNase ngesikhathi sokusetshenziswa.
Isincomo: Faka esikhundleni se-Viral DNA/RNA Isolation Kit entsha ukuze uthole ukuhlolwa okuhlobene.
5. Amashubhu e-centrifuge namathiphu e-piette asetshenziselwa ukukhwabanisa kwe-RNA angcoliswe yi-RNase.
Ukusikisela: Qinisekisa ukuthi amashubhu e-centrifuge, amathiphu e-pipette, amapayipi, njll. asetshenziselwa ukukhipha i-RNA konke Akuna-RNAse.
I-nucleic acid ehlanjululwe ithinta ukuhlolwa okungezansi komfula
I-DNA ne-RNA ehlanjululwe yikholomu yokuhlanza, uma i-ion kasawoti kanye nokuqukethwe kwamaprotheni kuphezulu kakhulu, kuzothinta ukuhlolwa okuya phansi komfula, okufana nalokhu: Ukukhulisa i-PCR, ukuloba okuhlanekezelwe, njll.
1. I-DNA ekhishiwe kanye ne-RNA inama-ion kasawoti asele.
Isiphakamiso: Qinisekisa ukuthi ivolumu elungile ye-ethanol ephelele yengezwa ku-Buffer RW2, futhi ugeze ikholomu yokuhlanza kabili ngesivinini sokuhlanganisa esishiwo emiyalweni yokusebenza;Yenza i-centrifugation ukuze unciphise ukungcoliswa kwe-ion kasawoti.
2. I-DNA ekhishiwe kanye ne-RNA inezinsalela ze-ethanol.
Ukusikisela: Ngemva kokuqinisekisa ukugeza nge-Buffer RW2, yenza i-tube centrifugation engenalutho ngesivinini se-centrifugation emiyalweni yokusebenza;uma kusekhona izinsalela ze-ethanol, ungakwazi ukufaka i-centrifuge ishubhu elingenalutho bese ulibeka endaweni yokushisa yasekamelweni imizuzu engu-5 ukuze ususe insalela ye-ethanol ngezinga elikhulu kakhulu.
Amamanuwali wemiyalo:
I-Viral DNA&RNA Isolation Kit Instruction Manual