Sizibophezele ekunikezeni ukusekelwa kokuthenga okulula, okonga isikhathi kanye nokonga kokukodwa komthengi kwekhithi yokukhipha i-nucleic acid, Samukela ngobuqotho abathengi baphesheya kwezilwandle ukuze babhekisele kukho ukuze bathole ukubambisana kwesikhathi eside kanye nenqubekela phambili efanayo.
Sizibophezele ekunikezeni uxhaso lokuthenga olulula, olusindisa isikhathi kanye nokonga imali okukodwa kwabathengiUkuhlolwa kwe-Nucleic Acid, Impela, intengo yokuncintisana, iphakheji efanelekile kanye nokulethwa okufika ngesikhathi kuzoqinisekiswa ngokwezidingo zamakhasimende.Sithemba ngobuqotho ukwakha ubuhlobo bebhizinisi nawe ngesisekelo sokuhlomula kanye nenzuzo esikhathini esizayo esiseduze.Siyakwamukela ngokufudumele ukuxhumana nathi futhi ube abahlanganyeli bethu abaqondile.

Imininingwane

50 Preps, 200 Preps

Izingxenye zekhithi

Izici&izinzuzo

■ Yonke inqubo iqhutshwa ekamelweni lokushisa (15-25 ℃), ngaphandle kokugeza kweqhwa kanye nokushisa okuphansi kwe-centrifugation.
■ Ikhithi yonke ayinayo i-RNase-Free, asikho isidingo sokukhathazeka ngokuwohloka kwe-RNA.
■ Ikholomu Yokuhlanza I-DNA ibopha ngokukhethekile i-DNA, ukuze ikhithi ikwazi ukususa ukungcola kwe-DNA ye-genomic ngaphandle kokwengeza i-DNase eyengeziwe.
■ Isivuno esikhulu se-RNA: Ikholomu ye-RNA kuphela kanye nefomula eyingqayizivele ingahlanza i-RNA ngokuphumelelayo.
■ Isivinini esisheshayo: kulula ukusebenza futhi singaqedwa ngemizuzu eyi-11.
■ Ukuphepha: Asikho i-reagent ephilayo edingekayo.
■ Ikhwalithi ephezulu: I-RNA ehlanziwe imsulwa kakhulu, ayinamaprotheni nokunye ukungcola, futhi ingahlangabezana nokuhlola okuhlukahlukene okulandelayo.

Isicelo sekhithi

Ilungele ukukhishwa nokuhlanzwa kwe-RNA ephelele kumaseli akhulisiwe kumapuleti angama-96, 24, 12, kanye namapuleti angu-6.

Ukugeleza komsebenzi

Umdwebo

Umdwebo webhethri yejeli ye-agarose ye-Cell Total RNA Isolation Kit iphathe izinombolo ezingenhla ezihlukene zamaseli, ukukhishwa kwevolumu engu-20μl, thatha u-2μl isamba esihlanziwe se-RNA 1%.

Isitoreji nempilo yeshelufu

Ikhithi ingagcinwa izinyanga eziyi-12 ekamelweni lokushisa (15-25 ℃) noma 2-8 ℃ isikhathi eside (izinyanga ezingama-24).

I-Buffer cRL1 ingagcinwa ku-4 ℃ inyanga engu-1 ngemva kokwengeza i-2-hydroxy-1-ethanethiol(ongakukhetha).

Sizibophezele ekunikezeni ukusekelwa kokuthenga okulula, okonga isikhathi kanye nokonga okukodwa komthengi we-OEM/ODM China China Nokonden Mobile ThermometricUkuhlolwa kwe-Nucleic AcidBooth, Samukela ngobuqotho abathengi baphesheya ukuthi babhekise kukho ukuze bathole ukubambisana kwesikhathi eside kanye nenqubekela phambili efanayo.
OEM/ODM China China Thermometric,Ukuhlolwa kwe-Nucleic Acid, Impela, intengo yokuncintisana, iphakheji efanelekile kanye nokulethwa okufika ngesikhathi kuzoqinisekiswa ngokwezidingo zamakhasimende.Sithemba ngobuqotho ukwakha ubuhlobo bebhizinisi nawe ngesisekelo sokuhlomula kanye nenzuzo esikhathini esizayo esiseduze.Siyakwamukela ngokufudumele ukuxhumana nathi futhi ube abahlanganyeli bethu abaqondile.