I-China Factory Supply 2 X Fast Pfus PCR Master Mix ne-Pfu DNA Polymerase Dntps PCR Buffer Umkhiqizi Nomhlinzeki |I-Foregene

I-Factory Supply 2 X Fast Pfus PCR Master Mix ene-Pfu DNA Polymerase Dntps PCR Buffer

Incazelo yekhithi:

Sebenzisa ngokuqondile i-polysaccharide ne-polyphenol leaf lysate njengesifanekiso sokukhulisa i-PCR, ngaphandle kokukhipha okuhlukile kwe-DNA, ukuzwela okusheshayo nokuphezulu.

◮Akukho ukuhlanzwa kwe-DNA okudla isikhathi nokubizayo (Isikhathi, umsebenzi nezindleko zilondoloziwe!)

◮Impahla encane

◮Kulula-isampula ingaba ngaphansi kokusabela kwe-PCR noma ukusabela kwe-lysis ngaphandle kokusika noma ukugaya

◮2 X Mix—ukunciphisa amaphutha okulayisha amasampula kanye nesikhathi sokulungiselela isistimu yokusabela

◮Ukulungiselela isifanekiso esisheshayo kungaqedwa emizuzwini eyi-10, ukusabela kwe-PCR kungaqedwa emizuzwini engama-50

◮Ukusabela okuphezulu kwe-throughput—lysis reaction kungaqedelwa kupuleti le-PCR elinemithombo engu-96

Thumela i-imeyili kithi Landa njenge-PDF

Imininingwane Yomkhiqizo

Omaka bomkhiqizo

Sigcizelela intuthuko futhi sethula imikhiqizo emisha emakethe unyaka ngamunye ye-Factory Supply 2 X Fast Pfus PCR Master Mix ene-Pfu DNA Polymerase Dntps PCR Buffer, Njengoba siqhubekela phambili, sibheka uhla lwemikhiqizo yethu ehlala ikhula futhi senza ngcono izinkampani zethu.
Sigcizelela intuthuko futhi sethula imikhiqizo emisha emakethe unyaka ngamunyeI-China PCR Master Mix ne-PCR Reagent, Sihlose ukuba ibhizinisi lesimanje elinomqondo omuhle wezohwebo “wobuqotho nokuzethemba” futhi ngenhloso “Yokunikeza amakhasimende amasevisi aqotho kakhulu nemikhiqizo yekhwalithi engcono kakhulu”.Sikucela ngobuqotho ukwesekwa kwakho okungaguquki futhi sibonga izeluleko nesiqondiso sakho esinomusa.

Imininingwane

50×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns

Izingxenye zekhithi

Ingxenye I	Buffer PS1
	Isilondolozi se-P2
	I-6×I-DNA Yokulayisha Ibhafa
Ingxenye II	Buffer PS2
Ingxenye II	I-2 × I-PCR yeLeaf Easy^TMHlanganisa

Izici&izinzuzo

■ Akukho ukuhlanzwa kwe-DNA okuchitha isikhathi nokubizayo
■ Impahla encane
■ Kulula-isampula ingaba ngaphansi kokusabela kwe-PCR noma ukusabela kwe-lysis ngaphandle kokusika noma ukugaya
■ 2 × Mix, ukunciphisa amaphutha okulayisha amasampula kanye nesikhathi sokulungiselela uhlelo lokusabela
■ Ukulungiselela isifanekiso esisheshayo kungaqedwa ngemizuzu eyi-10, ukusabela kwe-PCR kungaqedwa okungenani ngemizuzu engama-50
■ Ukusabela okuphezulu kwe-throughput-lysis kungaqedwa ngepuleti le-PCR le-96-well

Imingcele yekhithi

Isicelo: Ukuhlonza izitshalo ze-transgenic, i-genotyping, njll.

Isampula: amaqabunga esitshalo anothe nge-polysaccharide noma i-polyphenol

Usayizi wesampula: Ububanzi 2-3mm (indlela eqondile), ububanzi 5-7mm (indlela ye-lysis)

Ibanga lokutholwa: umkhiqizo we-PCR ≤1kb

Ukugeleza komsebenzi

plant leaf direct PCR plus-workflow

Electropherogram

plant leaf ngqo PCR plus Sigcizelela intuthuko futhi sethula imikhiqizo emisha emakethe unyaka ngamunye ye-Factory Supply 2 X Fast Pfus PCR Master Mix ene-Pfu DNA Polymerase Dntps PCR Buffer, Njengoba siqhubekela phambili, sibheka uhla lwemikhiqizo yethu ehlala ikhula futhi senza ngcono izinkampani zethu.
Ukunikezwa KwemboniI-China PCR Master Mix ne-PCR Reagent, Sihlose ukuba ibhizinisi lesimanje elinomqondo omuhle wezohwebo “wobuqotho nokuzethemba” futhi ngenhloso “Yokunikeza amakhasimende amasevisi aqotho kakhulu nemikhiqizo yekhwalithi engcono kakhulu”.Sikucela ngobuqotho ukwesekwa kwakho okungaguquki futhi sibonga izeluleko nesiqondiso sakho esinomusa.