Futhi sisebenza ngokukhethekile ekuthuthukiseni ukuphathwa kwezinto kanye nendlela ye-QC ukuze sikwazi ukugcina umkhawulo omkhulu ngaphakathi kwebhizinisi elincane elincintisana kakhulu ukuze uthole isampula Yamahhala ye-China CE/FDA Approved Magnetic Beads Method DNA / Rna Nucleic Acid Extraction Reagent Kits (I-Pre-packed Magnetic Beads), Ukubona ukuxhumana kwethu okuzuzisayo kwezitolo zethu phesheya kwezilwandle kuzuzisa umhlaba wonke. , ukulethwa okusheshayo, ikhwalithi enhle kakhulu nokubambisana kwesikhathi eside.
Futhi sisebenza ngokukhethekile ekuthuthukiseni ukuphathwa kwezinto kanye nendlela ye-QC ukuze sikwazi ukugcina umkhawulo omkhulu ngaphakathi kwebhizinisi elincane elincintisana kakhuluI-China Nucleic Acid, China Nucleic Acid Manufacturers, Ukukhiqizwa kwethu kuthunyelwe emazweni angaphezu kuka-30 nasezifundeni njengomthombo wokuqala onenani eliphansi.Samukela ngobuqotho amakhasimende asekhaya nakwamanye amazwe ukuthi eze azoxoxisana nathi ngebhizinisi.

Kits Izincazelo

50 Preps, 200 Preps

Le kit isebenzisa ikholomu ye-spin kanye nefomula eyakhiwe inkampani yethu, engakwazi ukukhipha i-RNA ephezulu kanye nekhwalithi ephezulu engqikithini yezilwane ezihlukahlukene ngokusebenza kahle okuphezulu.Ihlinzeka ngekholomu ye-DNA-Cleaning ephumelelayo, engakwazi ukuhlukanisa kalula futhi ikhangise i-DNA ye-genomic ku-supernatant kanye ne-tissue lysate, elula futhi elondoloza isikhathi;Ikholomu ye-RNA kuphela ingahlanganisa ngempumelelo i-RNA futhi ingacutshungulwa kanyekanye ngefomula eyingqayizivele Amasampuli amaningi.

Lonke uhlelo luyi-RNase-Free, ukuze i-RNA ekhishiwe ingonakalisiwe;I-Buffer RW1, i-Buffer RW2 isistimu yokugeza ibhafa, ukuze i-RNA etholiwe ingabi nawo amaprotheni, i-DNA, i-ion, kanye nokungcoliswa kwenhlanganisela yezinto eziphilayo.

Izingxenye zekhithi:

Izici&izinzuzo

■ Asikho isidingo sokukhathazeka ngokuwohloka kwe-RNA;lonke uhlelo luyi-RNase-Free
■ Khipha ngempumelelo i-DNA usebenzisa i-DNA-Cleaning Column
■ Khipha i-DNA ngaphandle kokwengeza i-DNase
■ Kulula-yonke imisebenzi iqedwa ekamelweni lokushisa
■ Ukusebenza okusheshayo kungaqedwa ngemizuzu engama-30
■ I-Safe-akukho reagent ephilayo edingekayo
■ Ukuhlanzeka okuphezulu -OD260/280≈1.8-2.1

Isicelo sekhithi

Ilungele ukukhishwa nokuhlanzwa kwe-RNA ephelele ezinhlobonhlobo zezicubu zezilwane ezintsha noma eziqandisiwe noma amaseli akhulisiwe.

Imingcele yomkhiqizo

■ Izinhlelo zokusebenza eziwela phansi: ukuhlanganiswa kwe-cDNA ye-first-strand, i-RT-PCR, i-molecular cloning, i-Northern Blot, njll.
■ Amasampula: izicubu zezilwane, amaseli akhulisiwe
■ Umthamo: Izicubu 10-20mg, Amaseli(2-5)×10⁶
■ Umthamo omkhulu wokubopha we-DNA wekholomu yokuhlanza: 80 μg
■ Ivolumu ye-Elution: 50-200 μl

Ukugeleza komsebenzi

Umdwebo

I-Animal Total RNA Isolation Kit iphathwe ama-20mg
Amasampula egundane amasha, thatha i-RNA ephelele engu-5% 1% ye-agar

I-Glycogel electrophoresis
1: Ubende 2: Izinso
3: Isibindi 4: Inhliziyo

Isitoreji nempilo yeshelufu

Ikhithi ingagcinwa izinyanga ezingama-24 ekamelweni lokushisa (15-25 ℃) noma 2–8 ℃ isikhathi eside.I-Buffer RL1 ingagcinwa ku-4 ℃ inyanga engu-1 ngemva kokwengeza i-β- mercaptoethanol (uyazikhethela). Futhi sisebenza ngokukhethekile ekuthuthukiseni izinto zokuphatha kanye nendlela ye-QC ukuze sikwazi ukugcina umkhawulo onzima ngaphakathi kwebhizinisi elincane eliqhudelana kakhulu ngesampula yamahhala ye-China CE/FDA Approved Magnetic Accleed Nuclear Beads Nuclear DNA (Magnetic Acculection DNA) izikhangiso), Ukuze uthole izinzuzo ezifanayo, ibhizinisi lethu lithuthukisa kabanzi amaqhinga ethu okubambisana kwembulunga yonke mayelana nokuxhumana nabathengi baphesheya, ukulethwa okusheshayo, ikhwalithi enhle kakhulu nokubambisana kwesikhathi eside.
Isampula yamahhala yeI-China Nucleic Acid, China Nucleic Acid Manufacturers, Ukukhiqizwa kwethu kuthunyelwe emazweni angaphezu kuka-30 nasezifundeni njengomthombo wokuqala onenani eliphansi.Samukela ngobuqotho amakhasimende asekhaya nakwamanye amazwe ukuthi eze azoxoxisana nathi ngebhizinisi.