Okuhlangenwe nakho okuchichima kakhulu kokuphatha amaphrojekthi kanye no-1 kuya kumodeli eyodwa yomhlinzeki kwenza ukubaluleka okuphezulu kokuxhumana kwenkampani kanye nokuqonda kwethu kalula lokho okulindele embonini ethengiswa kakhulu yase-China Magnetic Bead Method Nucleic Acid Extraction Kits Rna Isolation DNA Purification Kit Lab Reagent, Njengeqembu elinolwazi futhi samukela ama-oda aklanyelwe.Inhloso eyinhloko yenhlangano yethu iwukuthuthukisa inkumbulo eyanelisayo yabo bonke abathengi, futhi simise ubambiswano lwebhizinisi lwesikhathi eside lokuwina.
Okuhlangenwe nakho okuchichima kakhulu kokuphatha amaphrojekthi kanye no-1 kuya kumodeli eyodwa yomhlinzeki kwenza ukubaluleka okuphezulu kokuxhumana kwenkampani nokuqonda kwethu kalula lokho okulindeleUkuhlanzwa okuzenzakalelayo kwe-Nucleic Acid, I-China Universal Rna DNA Reagents, Ngamandla aqinisiwe kanye nekhredithi ethembeke kakhudlwana, sibe lapha ukuze sinikeze usizo amakhasimende ethu ngokunikeza ikhwalithi ephezulu kakhulu nesevisi, futhi sikwazisa ngobuqotho ukwesekwa kwenu.Sizozama ukulondoloza isithunzi sethu esihle njengomhlinzeki wempahla ohamba phambili emhlabeni.Uma unemibuzo noma ukuphawula, kufanele uxhumane nathi ngokukhululekile.

Imininingwane

50 Preps, 200 Preps

Ikhithi isebenzisa ikholomu yokuphotha kanye nefomula eyakhiwe yi-Foregene, engakhipha ngempumelelo i-RNA ephezulu nekhwalithi ephezulu ezicutshini zezitshalo ezihlukahlukene ezinokuqukethwe okuphezulu kwama-polysaccharides noma ama-polyphenols.Ihlinzeka ngekholomu ye-DNA-Cleaning engasusa kalula i-genomic DNA ku-supernatant kanye ne-tissue lysate.Ikholomu ye-RNA kuphela ingabopha ngempumelelo i-RNA.Ikhithi ingacubungula inani elikhulu lamasampuli ngesikhathi esisodwa.

Lonke uhlelo aluqukethe i-RNase, ngakho-ke i-RNA ehlanziwe ngeke yehliswe.I-Buffer PRW1 kanye ne-Buffer PRW2 ingaqinisekisa ukuthi i-RNA etholiwe ayingcoliswanga amaprotheni, i-DNA, ama-ion, nezinhlanganisela eziphilayo.

Izingxenye zekhithi

Izici&izinzuzo

■ Ukusebenza ekamelweni lokushisa (15-25℃) kuyo yonke inqubo, ngaphandle kokugeza eqhweni nokushisa okuphansi kwe-centrifugation.
■ Ikhithi ephelele ye-RNase-Free, asikho isidingo sokukhathazeka ngokuwohloka kwe-RNA.
■ Ifaneleka ngokukhethekile ukuhlanzwa kwe-RNA kumasampula ezitshalo ama-polysaccharides nama-polyphenols.
■ Ikholomu Yokuhlanza I-DNA ibophezela ngokukhethekile ku-DNA, ukuze ikhithi isuse ukungcoliswa kwe-DNA ye-genomic ngaphandle kokwengeza i-DNase.
■ Isivuno esikhulu se-RNA: Ikholomu ye-RNA kuphela kanye nefomula eyingqayizivele ingahlanza i-RNA ngokuphumelelayo.
■ Isivinini esisheshayo: kulula ukusebenza futhi singaqedwa phakathi nemizuzu engama-30.
■ Ukuphepha: asikho i-reagent ephilayo edingekayo.
■ Ikhwalithi ephezulu: Izingcezwana ze-RNA ezihlanziwe zimsulwa kakhulu, azinawo amaphrotheni nokunye ukungcola, futhi zingahlangabezana nezinhlelo zokuhlola ezihlukahlukene ezansi nomfula.

Imingcele yomkhiqizo

■ Izinhlelo zokusebenza eziwela phansi: ukuhlanganiswa kwe-cDNA ye-first-strand, i-RT-PCR, i-molecular cloning, i-Northern Blot, njll.
■ Isampula: Izicubu zezitshalo ezintsha noma eziqandisiwe zama-polysaccharides nama-polyphenols
■ Umthamo: 50mg izicubu zezitshalo
■ Umthamo omkhulu wokubopha we-RNA wekholomu yokuhlanza: 80 μg
■ Ivolumu ye-Elution: 50-200 μl

Isicelo sekhithi

Ilungele ukukhishwa kanye nokuhlanzwa kwe-RNA ephelele kumasampula ezicubu zezitshalo ezintsha noma eziqandisiwe (ikakhulukazi izicubu zamaqabunga ezitshalo ezintsha) ezinokuqukethwe okuphezulu kwe-polysaccharide kanye ne-polyphenol.

Ukugeleza komsebenzi

Umdwebo

I-Plant Total RNA Isolation Kit Plus icutshungulwe u-50mg wamaqabunga amasha e-polysaccharides nama-polyphenols, futhi i-RNA ehlanzekile engu-5% yahlolwa nge-electrophoresis.
1: Ubhanana
2: Ginkgo
3: Ukotini
4: Ihalanathi

Isitoreji kanye Nempilo yeshelufu

Le kit ingagcinwa izinyanga ezingu-24 ngaphansi kwezimo ezomile ekamelweni lokushisa (15-25℃);uma idinga ukugcinwa isikhathi eside, ingagcinwa ku-2–8℃.
I-Buffer PSL1 ingafakwa ku-4℃inyanga engu-1 ngemva kokwengeza i-β-mercaptoethanol (kuyanconywa ukuthi uyengeze ngesikhathi esifanayo sokuhlola).Okuhlangenwe nakho okuchichima kakhulu kokuphatha amaphrojekthi kanye noku-1 kuya kumodeli eyodwa yomhlinzeki kwenza ukubaluleka okuphezulu kokuxhumana kwenkampani nokuqonda kwethu okulula okulindelwe yi-Factory edayisa kakhulu i-China Magnetic Bead Actraction Method I-DNA Nuclear Experience iqembu futhi siyawamukela ama-oda enziwe ngendlela efanele.Inhloso eyinhloko yenhlangano yethu iwukuthuthukisa inkumbulo eyanelisayo yabo bonke abathengi, futhi simise ubambiswano lwebhizinisi lwesikhathi eside lokuwina.
Ifekthri edayisa kakhuluI-China Universal Rna DNA Reagents, Ukuhlanzwa okuzenzakalelayo kwe-Nucleic Acid, Ngamandla aqinisiwe kanye nekhredithi ethembeke kakhudlwana, sibe lapha ukuze sinikeze usizo amakhasimende ethu ngokunikeza ikhwalithi ephezulu kakhulu nesevisi, futhi sikwazisa ngobuqotho ukwesekwa kwenu.Sizozama ukulondoloza isithunzi sethu esihle njengomhlinzeki wempahla ohamba phambili emhlabeni.Uma unemibuzo noma ukuphawula, kufanele uxhumane nathi ngokukhululekile.