Cishe sinemishini ephumayo esezingeni eliphezulu kakhulu, onjiniyela abanolwazi nabaqeqeshiwe nabasebenzi, abahlonishwayo amasistimu okuphatha asezingeni eliphezulu kanye nokwesekwa kwabasebenzi abaholayo abanobungane ngaphambi/kwangemuva kokuthengisa kwe-CE Certificate China 16 Isolation Reagent Viral DNA/Rna Purification Kit, Siphinde sizingela ngokuqhubekayo ukuze sisungule ubudlelwano nabanye abahlinzeki bezitolo abasha ukuze sihlinzekele abahlinzeki bethu abathuthukayo.
Cishe sinemishini ephumayo esezingeni eliphezulu kakhulu, onjiniyela abanolwazi nabaqeqeshiwe nabasebenzi, amasistimu okuphatha asezingeni eliphezulu kanye nabasebenzi abanobungane abahola imali abasekela ngaphambi/ngemuva kokuthengisaIkhithi ye-Reagent ye-China Nucleic Acid Extraction, Kit Nucleic Acid Isizinda Reagent Kit, Sibambelele kukhwalithi enhle kakhulu, amanani ancintisanayo kanye nokulethwa kwesikhathi kanye nenkonzo engcono, futhi sithemba ngobuqotho ukusungula ubudlelwano obuhle besikhathi eside nokubambisana nozakwethu abasha nabandala bebhizinisi abavela kuwo wonke umhlaba.Siyakwamukela ngokuzithoba ukuthi uhlanganyele nathi.

Imininingwane

50 Preps, 200 Preps

Ikhithi isebenzisa ikholomu yokuphotha kanye nefomula eyakhiwe yi-Foregene, engakhipha ngempumelelo i-RNA ephezulu nekhwalithi ephezulu ezicutshini zezitshalo ezihlukahlukene ezinokuqukethwe okuphansi kwama-polysaccharides nama-polyphenols.Kumasampula ezitshalo ane-polysaccharides ephezulu noma okuqukethwe kwama-polyphenols, kunconyelwa ukusebenzisa i-Plant Total RNA Isolation Plus Kit ukuze uthole imiphumela engcono yokukhipha i-RNA.Ikhithi inikeza ikholomu ye-DNA-Cleaning engasusa kalula i-genomic DNA ku-supernatant kanye ne-tissue lysate.Ikholomu ye-RNA kuphela ingabopha ngempumelelo i-RNA.Ikhithi ingacubungula inani elikhulu lamasampuli ngesikhathi esisodwa.

Lonke uhlelo aluqukethe i-RNase, ngakho-ke i-RNA ehlanziwe ngeke yehliswe.I-Buffer PRW1 kanye ne-Buffer PRW2 ingaqinisekisa ukuthi i-RNA etholiwe ayingcoliswanga amaprotheni, i-DNA, ama-ion, nezinhlanganisela eziphilayo.

Izingxenye zekhithi

Izici&izinzuzo

■ Ukusebenza ekamelweni lokushisa (15-25℃) kuyo yonke inqubo, ngaphandle kokugeza eqhweni nokushisa okuphansi kwe-centrifugation.
■ Ikhithi ephelele ye-RNase-Free, asikho isidingo sokukhathazeka ngokuwohloka kwe-RNA.
■ Ikholomu Yokuhlanza I-DNA ibophezela ngokukhethekile ku-DNA, ukuze ikhithi isuse ukungcoliswa kwe-DNA ye-genomic ngaphandle kokwengeza i-DNase.
■ Isivuno esikhulu se-RNA: Ikholomu ye-RNA kuphela kanye nefomula eyingqayizivele ingahlanza i-RNA ngokuphumelelayo.
■ Isivinini esisheshayo: kulula ukusebenza futhi singaqedwa phakathi nemizuzu engama-30.
■ Ukuphepha: asikho i-reagent ephilayo edingekayo.
■ Ikhwalithi ephezulu: Izingcezwana ze-RNA ezihlanziwe zimsulwa kakhulu, azinawo amaphrotheni nokunye ukungcola, futhi zingahlangabezana nezinhlelo zokuhlola ezihlukahlukene ezansi nomfula.

Isicelo sekhithi

Ilungele ukukhishwa nokuhlanzwa kwe-RNA ephelele kumasampula ezicubu zezitshalo ezintsha noma eziqandisiwe (ikakhulukazi izicubu zamaqabunga ezitshalo ezintsha) ezine-polysaccharide ephansi kanye nokuqukethwe kwe-polyphenol.

Ukugeleza komsebenzi

Umdwebo

I-Plant Total RNA Isolation Kit Plus icutshungulwe u-50mg wamaqabunga amasha e-polysaccharides nama-polyphenols, futhi i-RNA ehlanzekile engu-5% yahlolwa nge-electrophoresis.
1: Ubhanana
2: Ginkgo
3: Ukotini
4: Ihalanathi

Isitoreji nempilo yeshelufu

Ikhithi ingagcinwa izinyanga eziyi-12 ekamelweni lokushisa (15-25 ℃) endaweni eyomile, kanye no-2-8 ℃ isikhathi eside (izinyanga ezingu-24).

I-Buffer PSL1 ingagcinwa ku-4 ℃ inyanga engu-1 ngemva kokwengeza i-2-hydroxy-1-ethanethiol(uyazikhethela). Cishe sinemishini ephumayo esezingeni eliphezulu kakhulu, onjiniyela abanolwazi nabaqeqeshiwe nabasebenzi, izinhlelo zokuphatha eziqashelwayo ezisezingeni eliphakeme kanye nabasebenzi abanobungane abaholayo abaholayo abanobungane ukwesekwa kwangaphambi/ngemuva kokuthengisa kwe-CE Certificate China/ReantiRing 16 ukwakha ubudlelwano nabahlinzeki abasha ukuze sihlinzekele abathengi bethu ababalulekile ngenye indlela eqhubekayo nehlakaniphile.
Isitifiketi se-CEIkhithi ye-Reagent ye-China Nucleic Acid Extraction, Kit Nucleic Acid Isizinda Reagent Kit, Sibambelele kukhwalithi enhle kakhulu, amanani ancintisanayo kanye nokulethwa kwesikhathi kanye nenkonzo engcono, futhi sithemba ngobuqotho ukusungula ubudlelwano obuhle besikhathi eside nokubambisana nozakwethu abasha nabandala bebhizinisi abavela kuwo wonke umhlaba.Siyakwamukela ngokuzithoba ukuthi uhlanganyele nathi.