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ukukhishwa kwe-nucleic acid okuzenzakalelayo

Incazelo yekhithi:

Le kit isebenzisa ubuchwepheshe be-Real-time RT PCR(rRT-PCR) ukuze kutholwe ikhwalithi ye-SARS-CoV-2 (ORF1ab gene ne-N gene) amasampula e-nasopharyngeal noma i-oropharyngeal swab, nge-multiplex PCR fluorescent probe method. ukutholwa ngokushesha okukhulu.


Imininingwane Yomkhiqizo

Omaka bomkhiqizo

Siphishekela imfundiso yokuphatha ethi “Ikhwalithi iyingqayizivele, Umhlinzeki uphakeme, Igama lingowokuqala”, futhi sizodala futhi sabelane ngempumelelo nawo wonke amakhasimende ukuze kukhishwe i-nucleic acid okuzenzakalelayo, Sikholelwa ukuthi insizakalo yethu efudumele neyobuchwepheshe izokulethela izinto ezimangalisayo ezingalindelekile kanye nenhlanhla.
Siphishekela imfundiso yokuphatha ethi "Ikhwalithi iyingqayizivele, Umhlinzeki uphakeme, Igama lingowokuqala", futhi sizodala futhi sabelane ngempumelelo nawo wonke amakhasimendeI-China DNA/Rna Nucleic Acid Extraction, I-Nucleic Acid Isizinda System, Sinolwazi olwanele lokukhiqiza imikhiqizo ngokuya ngamasampula noma imidwebo.Samukela ngokufudumele amakhasimende asekhaya nakwamanye amazwe ukuthi avakashele inkampani yethu, futhi abambisane nathi ukuze sibe nekusasa elihle ndawonye.


Imininingwane

48 Rxns, 96 Rxns

Cha.

Isakhi

Inani

Izingxenye eziyinhloko

awu 48r

awu 96r

1 I-ejenti yokukhululwa kwe-Nucleic acid 1.4mL/ithubhu 2 amashubhu 5.3 mL/ibhodlela 1 ibhodlela I-surfactant
2 Isivikeli se-RNA 27 μL/ithubhu 1 ithubhu 53 μL/ithubhu 1 ithubhu I-RNase inhibitor
3 Isixazululo sokusabela sakwa-SARS-CoV-2 800 μL / ishubhu 1 ithubhu 1600 μL/ipayipi 1 ithubhu I-Primer, probe, buffer yokusabela, i-dNTP
4 Ingxube ye-enzyme ye-SARS-CoV-2 80 μL/ithubhu 1 ithubhu 160 μL/ishubhu 1 ithubhu Isiqalo esishisayo se-Taq enzyme, i-enzyme ye-M-MLV
5 Ukulawula okuhle kwe-SARS-CoV-2 100 μL / ishubhu 1 ithubhu 100 μL / ishubhu 1 ithubhu I-plasmid ehlanganisiwe equkethe ingxenye yethagethi, i-RNA
6 Ukulawula okungekuhle kwe-SARS-CoV-2 1200 μL / ishubhu 1 ithubhu 1200 μL / ishubhu 1 ithubhu Ibhafa ye-TE

Izici&izinzuzo

Ngokushesha futhi kulula.
Gwema ukulahleka kwe-nucleic acid.
Umkhawulo wokutholwa ophansi nokuzwela okuphezulu.
Isidingo semishini ephansi kanye nokusetshenziswa okuvumelana nezimo kakhulu.

Ukuzwela kokutholwa kungafinyelela ezingeni eliphansi kunawo wonke njengamakhophi angu-500/mL).

I-GCE/mL Inani le-CT
I-N isakhi I-ORF1ab yofuzo
2.00E+08 16.43 15.16
2.00E+07 19.48 18.58
2.00E+06 22.71 21.86
2.00E+05 25.95 25.33
2.00E+04 29.30 28.49
2.00E+03 32.37 31.37
2.00E+02 35.32 34.47
2.00E+01 38.29 38.27
2.00E+00 N/A N/A
2.00E-01 N/A N/A
NC N/A N/A

Ijika lokukhulisa umugqa

ijika le-amplification-RT-qPCR

Isetshenziswa kanjani

1. Ithuluzi elisebenzayo

ikhithi yokuthola i-covid-19 nucleic acid1

2. Inqubo yokuthola

i-covid-19 nucleic acid kit2

Ukucutshungulwa kwesampula:Isampula ye-Naopharyngeal noma i-oropharyngeal swab (isinyathelo esi-1 kuphela sokusebenza)(Linda imizuzu eyi-10)

I-PCR amplificaton(<=55min)

Ukuhamba komsebenzi:

i-covid-19 nucleic acid kit4

Isitoreji

ivalwe ekukhanyeni futhi igcinwe ku -20±5℃;

isitoreji
isitoreji2

Impilo yeshelufu:1 unyaka

Siphishekela inkambiso yokuphatha ethi “Ikhwalithi iyingqayizivele, Umhlinzeki uphakeme, Igama lingowokuqala”, futhi sizodala futhi sabelane ngempumelelo nawo wonke amakhasimende e-PriceList ye-China Biobase PCR Laboratory DNA/Rna Nucleic Acid Extraction (Betsy), Sikholelwa ukuthi insizakalo yethu efudumele nobungcweti izokulethela lezimanga ezimnandi kanye nenhlanhla.
PriceList forI-China DNA/Rna Nucleic Acid Extraction, I-Nucleic Acid Isizinda System, Sinolwazi olwanele lokukhiqiza imikhiqizo ngokuya ngamasampula noma imidwebo.Samukela ngokufudumele amakhasimende asekhaya nakwamanye amazwe ukuthi avakashele inkampani yethu, futhi abambisane nathi ukuze sibe nekusasa elihle ndawonye.


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