Siphishekela inkambiso yokuphatha ethi “Ikhwalithi isezingeni eliphezulu, Izinkonzo ziphakeme, ukuduma kuqala”, futhi sizodala futhi sabelane ngempumelelo nawo wonke amaklayenti ngentengo yokuthengisa ka-2019 China Darui Disposal Detection Kits Sample Release Reagent Needless Rna/ Nucleic Acid Extraction Kit Reagent Kits Isikhathi Sangempela, Sibheke phambili ukubambisana kwabathengi ngisho phesheya kwezilwandle.Uma ngabe uthakasela cishe kunoma yisiphi isixazululo sethu, khumbula ukuzwa ukhululeke ngokuphelele ukukhuluma nathi ukuze uthole amaqiniso engeziwe.
Siphishekela imfundiso yokuphatha ethi “Ikhwalithi isezingeni eliphezulu, Izinkonzo ziphakeme, ukuthandwa kuqala”, futhi sizodala futhi sabelane ngempumelelo nawo wonke amakhasimendeI-Reagent Yokukhishwa Kwesampula yase-China, I-Reagent yokukhipha, Ngokusekelwe emugqeni wethu wokukhiqiza ozenzakalelayo, isiteshi sokuthenga izinto ezibonakalayo ezingashintshi kanye nezinhlelo ezisheshayo zenkontileka engaphansi kwakhiwe ezweni laseChina ukuze kuhlangatshezwane nemfuneko ebanzi nephakeme yamakhasimende eminyakeni yamuva.Besilokhu sibheke ngabomvu ukubambisana namakhasimende engeziwe emhlabeni wonke ukuze sithuthuke ngokufanayo futhi sizuze ngokufanayo!Ukwethemba kwakho nokugunyazwa kwakho kuwumvuzo ongcono kakhulu wemizamo yethu.Ngokuhlala sithembekile, sisungula izinto ezintsha futhi sisebenza kahle, silindele ngobuqotho ukuthi singaba ozakwethu bebhizinisi ukuze sakhe ikusasa lethu eliqhakazile!

Incazelo

50 Preps, 200 Preps

Le khithi isebenzisa ikholomu yokuphotha kanye nefomula eyakhiwe yi-Foregene, engakhipha ngempumelelo i-RNA ephezulu nekhwalithi ephezulu kumaseli akhuliswe ngamapuleti emithombo engu-96, 24, 12, kanye ne-6.

Ikhithi inikeza Ikholomu Yokuhlanza I-DNA esebenza kahle, engahlukanisa kalula i-supernatant ne-cell lysate, ibophe futhi isuse i-genomic DNA.Ukusebenza kulula futhi konga isikhathi.

Ikholomu ye-RNA kuphela ingahlanganisa ngempumelelo i-RNA ngefomula ehlukile.Inani elikhulu lamasampula lingacutshungulwa kanyekanye.

Izingxenye zekhithi

*Sicela ugqoke amagilavu ​​futhi uthathe izinyathelo zokuvikela ngesikhathi sokusebenza njengoba i-Buffer cRL1 ne-Buffer RW1 iqukethe usawoti we-chaotropic ocasulayo.

Izici&izinzuzo

■ Yonke inqubo iqhutshwa ekamelweni lokushisa (15-25 ℃), ngaphandle kokugeza kweqhwa kanye nokushisa okuphansi kwe-centrifugation.
■ Ikhithi yonke ayinayo i-RNase-Free, asikho isidingo sokukhathazeka ngokuwohloka kwe-RNA.
■ Ikholomu Yokuhlanza I-DNA ibopha ngokukhethekile i-DNA, ukuze ikhithi ikwazi ukususa ukungcola kwe-DNA ye-genomic ngaphandle kokwengeza i-DNase eyengeziwe.
■ Isivuno esikhulu se-RNA: Ikholomu ye-RNA kuphela kanye nefomula eyingqayizivele ingahlanza i-RNA ngokuphumelelayo.
■ Isivinini esisheshayo: kulula ukusebenza futhi singaqedwa ngemizuzu eyi-11.
■ Ukuphepha: Asikho i-reagent ephilayo edingekayo.
■ Ikhwalithi ephezulu: I-RNA ehlanziwe imsulwa kakhulu, ayinamaprotheni nokunye ukungcola, futhi ingahlangabezana nokuhlola okuhlukahlukene okulandelayo.

Isicelo sekhithi

Ilungele ukukhishwa nokuhlanzwa kwe-RNA ephelele kumaseli akhulisiwe kumapuleti angama-96, 24, 12, kanye namapuleti angu-6.

Ukugeleza komsebenzi

Umdwebo

Umdwebo webhethri yejeli ye-agarose ye-Cell Total RNA Isolation Kit iphathe izinombolo ezingenhla ezihlukene zamaseli, ukukhishwa kwevolumu engu-20μl, thatha u-2μl isamba esihlanziwe se-RNA 1%.

Isitoreji nempilo yeshelufu

Ikhithi ingagcinwa izinyanga eziyi-12 ekamelweni lokushisa (15-25 ℃) noma 2-8 ℃ isikhathi eside (izinyanga ezingama-24).

I-Buffer cRL1 ingagcinwa ku-4 ℃ inyanga engu-1 ngemva kokwengeza i-2-hydroxy-1-ethanethiol(ongakukhetha).

Siphishekela inkambiso yokuphatha ethi “Ikhwalithi isezingeni eliphezulu, Izinkonzo ziphakeme, ukuduma kuqala”, futhi sizodala futhi sabelane ngempumelelo nawo wonke amaklayenti ngentengo yokuthengisa ka-2019 China Darui Disposal Detection Kits Sample Release Reagent Needless Rna/ Nucleic Acid Extraction Kit Reagent Kits Isikhathi Sangempela, Sibheke phambili ukubambisana kwabathengi ngisho phesheya kwezilwandle.Uma ngabe uthakasela cishe kunoma yisiphi isixazululo sethu, khumbula ukuzwa ukhululeke ngokuphelele ukukhuluma nathi ukuze uthole amaqiniso engeziwe.
Intengo ye-wholesale yango-2019I-Reagent Yokukhishwa Kwesampula yase-China, I-Reagent yokukhipha, Ngokusekelwe emugqeni wethu wokukhiqiza ozenzakalelayo, isiteshi sokuthenga izinto ezibonakalayo ezingashintshi kanye nezinhlelo ezisheshayo zenkontileka engaphansi kwakhiwe ezweni laseChina ukuze kuhlangatshezwane nemfuneko ebanzi nephakeme yamakhasimende eminyakeni yamuva.Besilokhu sibheke ngabomvu ukubambisana namakhasimende engeziwe emhlabeni wonke ukuze sithuthuke ngokufanayo futhi sizuze ngokufanayo!Ukwethemba kwakho nokugunyazwa kwakho kuwumvuzo ongcono kakhulu wemizamo yethu.Ngokuhlala sithembekile, sisungula izinto ezintsha futhi sisebenza kahle, silindele ngobuqotho ukuthi singaba ozakwethu bebhizinisi ukuze sakhe ikusasa lethu eliqhakazile!