Ukwaneliseka kwekhasimende kuyinhloso yethu eyinhloko.Siphakamisa izinga elingaguquki lobungcweti, ikhwalithi, ukwethembeka kanye nensizakalo Yabathengisi Bezitolo Ezithengisa Izimpahla Zase-China Hipure Viral Rna Kit Nucleic Acid Isolation & Purification Kit, Kunoma ubani othakasela noma yiziphi izinto, qiniseka ukuthi uzizwa ekhululekile ngempela ukukhuluma nathi ukuze uthole imininingwane eyengeziwe noma uqiniseke ukuthi usithumele i-imeyili ngokushesha, sizokuphendula futhi ngemva kwamahora angu-24 cishe uzonikezwa.
Ukwaneliseka kwekhasimende kuyinhloso yethu eyinhloko.Siphakamisa izinga elingaguquki lobuchwepheshe, ikhwalithi, ukwethembeka kanye nenkonzoI-China Rna/DNA Extraction/Purification, I-Viral Rna Isolation, Ukwaneliseka kweKhasimende kuwumgomo wethu.Sibheke ngabomvu ukubambisana nawe nokuhlinzeka ngezinsizakalo zethu ezihamba phambili endabeni yakho.Siyakwamukela ngokufudumele ukuthi usithinte futhi ukhumbule ukuthi ukhululeke ukuxhumana nathi.Phequlula igumbi lethu lokubonisa ku-inthanethi ukuze ubone ukuthi singakwenzela ini.Bese usithumela i-imeyili imininingwane yakho noma imibuzo namuhla.

Imininingwane

50 Preps, 200 Preps

Ikhithi isebenzisa ikholomu yokuphotha kanye nefomula eyakhiwe yi-Foregene, engakhipha ngempumelelo i-RNA ephezulu nekhwalithi ephezulu ezicutshini zezitshalo ezihlukahlukene ezinokuqukethwe okuphansi kwama-polysaccharides nama-polyphenols.Kumasampula ezitshalo ane-polysaccharides ephezulu noma okuqukethwe kwama-polyphenols, kunconyelwa ukusebenzisa i-Plant Total RNA Isolation Plus Kit ukuze uthole imiphumela engcono yokukhipha i-RNA.Ikhithi inikeza ikholomu ye-DNA-Cleaning engasusa kalula i-genomic DNA ku-supernatant kanye ne-tissue lysate.Ikholomu ye-RNA kuphela ingabopha ngempumelelo i-RNA.Ikhithi ingacubungula inani elikhulu lamasampuli ngesikhathi esisodwa.

Lonke uhlelo aluqukethe i-RNase, ngakho-ke i-RNA ehlanziwe ngeke yehliswe.I-Buffer PRW1 kanye ne-Buffer PRW2 ingaqinisekisa ukuthi i-RNA etholiwe ayingcoliswanga amaprotheni, i-DNA, ama-ion, nezinhlanganisela eziphilayo.

Izingxenye zekhithi

Izici&izinzuzo

■ Ukusebenza ekamelweni lokushisa (15-25℃) kuyo yonke inqubo, ngaphandle kokugeza eqhweni nokushisa okuphansi kwe-centrifugation.
■ Ikhithi ephelele ye-RNase-Free, asikho isidingo sokukhathazeka ngokuwohloka kwe-RNA.
■ Ikholomu Yokuhlanza I-DNA ibophezela ngokukhethekile ku-DNA, ukuze ikhithi isuse ukungcoliswa kwe-DNA ye-genomic ngaphandle kokwengeza i-DNase.
■ Isivuno esikhulu se-RNA: Ikholomu ye-RNA kuphela kanye nefomula eyingqayizivele ingahlanza i-RNA ngokuphumelelayo.
■ Isivinini esisheshayo: kulula ukusebenza futhi singaqedwa phakathi nemizuzu engama-30.
■ Ukuphepha: asikho i-reagent ephilayo edingekayo.
■ Ikhwalithi ephezulu: Izingcezwana ze-RNA ezihlanziwe zimsulwa kakhulu, azinawo amaphrotheni nokunye ukungcola, futhi zingahlangabezana nezinhlelo zokuhlola ezihlukahlukene ezansi nomfula.

Isicelo sekhithi

Ilungele ukukhishwa nokuhlanzwa kwe-RNA ephelele kumasampula ezicubu zezitshalo ezintsha noma eziqandisiwe (ikakhulukazi izicubu zamaqabunga ezitshalo ezintsha) ezine-polysaccharide ephansi kanye nokuqukethwe kwe-polyphenol.

Ukugeleza komsebenzi

Umdwebo

I-Plant Total RNA Isolation Kit Plus icutshungulwe u-50mg wamaqabunga amasha e-polysaccharides nama-polyphenols, futhi i-RNA ehlanzekile engu-5% yahlolwa nge-electrophoresis.
1: Ubhanana
2: Ginkgo
3: Ukotini
4: Ihalanathi

Isitoreji nempilo yeshelufu

Ikhithi ingagcinwa izinyanga eziyi-12 ekamelweni lokushisa (15-25 ℃) endaweni eyomile, kanye no-2-8 ℃ isikhathi eside (izinyanga ezingu-24).

I-Buffer PSL1 ingagcinwa ku-4 ℃ inyanga engu-1 ngemva kokwengeza i-2-hydroxy-1-ethanethiol(ongakukhetha).Ukwaneliseka kwekhasimende kuyinhloso yethu eyinhloko.Siphakamisa izinga elingaguquki lobungcweti, ikhwalithi, ukwethembeka kanye nensizakalo Yabathengisi Bezitolo Ezithengisa Izimpahla Zase-China Hipure Viral Rna Kit Nucleic Acid Isolation & Purification Kit, Kunoma ubani othakasela noma yiziphi izinto, qiniseka ukuthi uzizwa ekhululekile ngempela ukukhuluma nathi ukuze uthole imininingwane eyengeziwe noma uqiniseke ukuthi usithumele i-imeyili ngokushesha, sizokuphendula futhi ngemva kwamahora angu-24 cishe uzonikezwa.
Abathengisi bezitolo ezinkulu zeI-China Rna/DNA Extraction/Purification, I-Viral Rna Isolation, Ukwaneliseka kweKhasimende kuwumgomo wethu.Sibheke ngabomvu ukubambisana nawe nokuhlinzeka ngezinsizakalo zethu ezihamba phambili endabeni yakho.Siyakwamukela ngokufudumele ukuthi usithinte futhi ukhumbule ukuthi ukhululeke ukuxhumana nathi.Phequlula igumbi lethu lokubonisa ku-inthanethi ukuze ubone ukuthi singakwenzela ini.Bese usithumela i-imeyili imininingwane yakho noma imibuzo namuhla.