Sinezisebenzi ezisebenza kahle kakhulu ukubhekana nemibuzo evela kumakhasimende.Umgomo wethu "u-100% wenjabulo yekhasimende ngesixazululo sethu sekhwalithi enhle, inani kanye nesevisi yethu yeqembu" futhi sithanda irekhodi elihle kakhulu phakathi kwabathengi.Ngamafekthri amaningi, sizokwethula Imikhiqizo Ethrendayo eyahlukahlukene yase-China Techstar Rna DNA Extraction Kit Extraction Nucleic Acid Extraction Kit, Ilebhu Yethu manje “iyiLabhu Kazwelonke yobuchwepheshe benjini kadizili turbo ”, futhi singabanikazi be-R&D abaqeqeshiwe kanye nendawo yokuhlola ephelele.
Sinezisebenzi ezisebenza kahle kakhulu ukubhekana nemibuzo evela kumakhasimende.Umgomo wethu "u-100% wenjabulo yekhasimende ngesixazululo sethu sekhwalithi enhle, inani kanye nesevisi yethu yeqembu" futhi sithanda irekhodi elihle kakhulu phakathi kwabathengi.Ngamafekthri amaningi, sizokwethula okuhlukahlukene okuhlukahlukeneI-China Nucleic Acid Reagent, Ikhithi yokukhipha i-DNA/Rna, Ithini intengo enhle?Sihlinzeka amakhasimende ngentengo yefekthri.Ngokwesisekelo sekhwalithi enhle, ukusebenza kahle kuyodingeka kunakwe futhi kugcinwe inzuzo efanele ephansi nenempilo.Kuyini ukulethwa okusheshayo?Senza ukulethwa ngokwezidingo zamakhasimende.Nakuba isikhathi sokulethwa sincike enanini le-oda kanye nokuba yinkimbinkimbi kwalo, sisazama ukuhlinzeka ngemikhiqizo nezisombululo ngesikhathi.Ngethemba ukuthi singaba nobudlelwano bebhizinisi besikhathi eside.

Imininingwane

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Izingxenye zekhithi

Izici&izinzuzo

■ Yonke inqubo iqhutshwa ekamelweni lokushisa (15-25 ℃), ngaphandle kokugeza kweqhwa kanye nokushisa okuphansi kwe-centrifugation.
■ Ikhithi yonke ayinayo i-RNase-Free, asikho isidingo sokukhathazeka ngokuwohloka kwe-RNA.
■ Ikholomu Yokuhlanza I-DNA ibopha ngokukhethekile i-DNA, ukuze ikhithi ikwazi ukususa ukungcola kwe-DNA ye-genomic ngaphandle kokwengeza i-DNase eyengeziwe.
■ Isivuno esikhulu se-RNA: Ikholomu ye-RNA kuphela kanye nefomula eyingqayizivele ingahlanza i-RNA ngokuphumelelayo.
■ Isivinini esisheshayo: kulula ukusebenza futhi singaqedwa ngemizuzu eyi-11.
■ Ukuphepha: Asikho i-reagent ephilayo edingekayo.
■ Ikhwalithi ephezulu: I-RNA ehlanziwe imsulwa kakhulu, ayinamaprotheni nokunye ukungcola, futhi ingahlangabezana nokuhlola okuhlukahlukene okulandelayo.

Isicelo sekhithi

Ilungele ukukhishwa nokuhlanzwa kwe-RNA ephelele kumaseli akhulisiwe kumapuleti angama-96, 24, 12, kanye namapuleti angu-6.

Ukugeleza komsebenzi

Umdwebo

Umdwebo webhethri yejeli ye-agarose ye-Cell Total RNA Isolation Kit iphathe izinombolo ezingenhla ezihlukene zamaseli, ukukhishwa kwevolumu engu-20μl, thatha u-2μl isamba esihlanziwe se-RNA 1%.

Isitoreji nempilo yeshelufu

Ikhithi ingagcinwa izinyanga eziyi-12 ekamelweni lokushisa (15-25 ℃) noma 2-8 ℃ isikhathi eside (izinyanga ezingama-24).

I-Buffer cRL1 ingagcinwa ku-4 ℃ inyanga engu-1 ngemva kokwengeza i-2-hydroxy-1-ethanethiol(ongakukhetha).

Sinezisebenzi ezisebenza kahle kakhulu ukubhekana nemibuzo evela kumakhasimende.Umgomo wethu "u-100% wenjabulo yekhasimende ngesixazululo sethu sekhwalithi enhle, inani kanye nesevisi yethu yeqembu" futhi sithanda irekhodi elihle kakhulu phakathi kwabathengi.Ngamafekthri amaningi, sizokwethula Imikhiqizo Ethrendayo eyahlukahlukene yase-China Techstar Rna DNA Extraction Kit Extraction Nucleic Acid Extraction Kit, Ilebhu Yethu manje “iyiLabhu Kazwelonke yobuchwepheshe benjini kadizili turbo ”, futhi singabanikazi be-R&D abaqeqeshiwe kanye nendawo yokuhlola ephelele.
Imikhiqizo EthrendayoI-China Nucleic Acid Reagent, Ikhithi yokukhipha i-DNA/Rna, Ithini intengo enhle?Sihlinzeka amakhasimende ngentengo yefekthri.Ngokwesisekelo sekhwalithi enhle, ukusebenza kahle kuyodingeka kunakwe futhi kugcinwe inzuzo efanele ephansi nenempilo.Kuyini ukulethwa okusheshayo?Senza ukulethwa ngokwezidingo zamakhasimende.Nakuba isikhathi sokulethwa sincike enanini le-oda kanye nokuba yinkimbinkimbi kwalo, sisazama ukuhlinzeka ngemikhiqizo nezisombululo ngesikhathi.Ngethemba ukuthi singaba nobudlelwano bebhizinisi besikhathi eside.