Sinalesi siqubulo engqondweni, ngokuqinisekile sesingomunye wabakhiqizi abathuthuke kakhulu kwezobuchwepheshe, abangabizi kakhulu, nabaqhudelana namanani eBangeni Eliphezulu.I-China Nucleic Acid Extractionnoma indlela yekholomu ye-Purification Kit, Manje sesineminyaka engaphezu kwe-10 sisebenza.Sizinikele emikhiqizweni nezisombululo zekhwalithi ephezulu kanye nosizo lwabathengi.Sikumema ukuthi nakanjani uvakashele ibhizinisi lethu ukuze uthole ukuvakasha komuntu siqu kanye nesiqondiso senkampani esithuthukisiwe.
Sinalesi siqubulo emqondweni, sesingomunye wabakhiqizi abathuthuke kakhulu kwezobuchwepheshe, abangabizi kakhulu, futhi abancintisana namanani entengo.I-China Nucleic Acid Extraction, spin ikholomu Indlela, Sakhe ubudlelwano obuqinile nobude bokusebenzisana nenani elikhulu lezinkampani ezingaphakathi kwaleli bhizinisi phesheya kwezilwandle.Isevisi esheshayo nekhethekile emva kokuthengisa ehlinzekwa yiqembu lethu labaxhumanisi ithokozise abathengi bethu.Ngokujulile Ulwazi namapharamitha avela kokuthengiswayo cishe azothunyelwa kuwe ukuze uthole noma yikuphi ukuvuma okujulile.Amasampula wamahhala angase alethwe futhi inkampani ihlole enkampanini yethu.n I-Portugal yezingxoxo yamukelekile njalo.Ngethemba lokuthola imibuzo ukuthayipha bese wakhe ubambiswano lwesikhathi eside.

Imininingwane

50 Preps, 200 Preps

Ikhithi isebenzisa ikholomu yokuphotha kanye nefomula eyakhiwe yi-Foregene, engakhipha ngempumelelo i-RNA ephezulu nekhwalithi ephezulu ezicutshini zezitshalo ezihlukahlukene ezinokuqukethwe okuphansi kwama-polysaccharides nama-polyphenols.Kumasampula ezitshalo ane-polysaccharides ephezulu noma okuqukethwe kwama-polyphenols, kunconyelwa ukusebenzisa i-Plant Total RNA Isolation Plus Kit ukuze uthole imiphumela engcono yokukhipha i-RNA.Ikhithi inikeza ikholomu ye-DNA-Cleaning engasusa kalula i-genomic DNA ku-supernatant kanye ne-tissue lysate.Ikholomu ye-RNA kuphela ingabopha ngempumelelo i-RNA.Ikhithi ingacubungula inani elikhulu lamasampuli ngesikhathi esisodwa.

Lonke uhlelo aluqukethe i-RNase, ngakho-ke i-RNA ehlanziwe ngeke yehliswe.I-Buffer PRW1 kanye ne-Buffer PRW2 ingaqinisekisa ukuthi i-RNA etholiwe ayingcoliswanga amaprotheni, i-DNA, ama-ion, nezinhlanganisela eziphilayo.

Izingxenye zekhithi

Izici&izinzuzo

■ Ukusebenza ekamelweni lokushisa (15-25℃) kuyo yonke inqubo, ngaphandle kokugeza eqhweni nokushisa okuphansi kwe-centrifugation.
■ Ikhithi ephelele ye-RNase-Free, asikho isidingo sokukhathazeka ngokuwohloka kwe-RNA.
■ Ikholomu Yokuhlanza I-DNA ibophezela ngokukhethekile ku-DNA, ukuze ikhithi isuse ukungcoliswa kwe-DNA ye-genomic ngaphandle kokwengeza i-DNase.
■ Isivuno esikhulu se-RNA: Ikholomu ye-RNA kuphela kanye nefomula eyingqayizivele ingahlanza i-RNA ngokuphumelelayo.
■ Isivinini esisheshayo: kulula ukusebenza futhi singaqedwa phakathi nemizuzu engama-30.
■ Ukuphepha: asikho i-reagent ephilayo edingekayo.
■ Ikhwalithi ephezulu: Izingcezwana ze-RNA ezihlanziwe zimsulwa kakhulu, azinawo amaphrotheni nokunye ukungcola, futhi zingahlangabezana nezinhlelo zokuhlola ezihlukahlukene ezansi nomfula.

Isicelo sekhithi

Ilungele ukukhishwa nokuhlanzwa kwe-RNA ephelele kumasampula ezicubu zezitshalo ezintsha noma eziqandisiwe (ikakhulukazi izicubu zamaqabunga ezitshalo ezintsha) ezine-polysaccharide ephansi kanye nokuqukethwe kwe-polyphenol.

Ukugeleza komsebenzi

Umdwebo

I-Plant Total RNA Isolation Kit Plus icutshungulwe u-50mg wamaqabunga amasha e-polysaccharides nama-polyphenols, futhi i-RNA ehlanzekile engu-5% yahlolwa nge-electrophoresis.
1: Ubhanana
2: Ginkgo
3: Ukotini
4: Ihalanathi

Isitoreji nempilo yeshelufu

Ikhithi ingagcinwa izinyanga eziyi-12 ekamelweni lokushisa (15-25 ℃) endaweni eyomile, kanye no-2-8 ℃ isikhathi eside (izinyanga ezingu-24).

I-Buffer PSL1 ingagcinwa ku-4 ℃ inyanga engu-1 ngemva kokwengeza i-2-hydroxy-1-ethanethiol(ongakukhetha). Unalesi siqubulo engqondweni, ngokuqinisekile sesingomunye wabakhiqizi abaqambe kakhulu ubuchwepheshe, abangabizi kakhulu, nabaqhudelana namanani eBangeni Eliphezulu.I-China Nucleic Acid Extractionnoma Ikhithi Yokuhlanza (Indlela Yobuhlalu Bebuhlalu), Manje sesineminyaka engaphezu kweyi-10 sisebenza.Sizinikele emikhiqizweni nezisombululo zekhwalithi ephezulu kanye nosizo lwabathengi.Sikumema ukuthi nakanjani uvakashele ibhizinisi lethu ukuze uthole ukuvakasha komuntu siqu kanye nesiqondiso senkampani esithuthukisiwe.
IBanga Eliphezulu Le-China Nucleic Acid Extraction, Indlela Yobuhlalu Bezibuthe, Sakhe ubudlelwano obuqinile nobude bokusebenzisana nenani elikhulu lezinkampani ezikuleli bhizinisi phesheya kwezilwandle.Isevisi esheshayo nekhethekile emva kokuthengisa ehlinzekwa yiqembu lethu labaxhumanisi ithokozise abathengi bethu.Ngokujulile Ulwazi namapharamitha avela kokuthengiswayo cishe azothunyelwa kuwe ukuze uthole noma yikuphi ukuvuma okujulile.Amasampula wamahhala angase alethwe futhi inkampani ihlole enkampanini yethu.n I-Portugal yezingxoxo yamukelekile njalo.Ngethemba lokuthola imibuzo ukuthayipha bese wakhe ubambiswano lwesikhathi eside.