Siyaqhubeka nomoya wethu webhizinisi "wekhwalithi, ukusebenza, ukusungula izinto ezintsha kanye nobuqotho".Sihlose ukudala inani elithe xaxa kubathengi bethu ngezinsiza zethu ezilayishiwe, imishini yesimanjemanje, abasebenzi abanolwazi nezinsizakalo ezinhle zochwepheshe Zamanani Akhethekile e-China Real-Time Quantitative PCR Thermal Cycler enesikrini sokuthinta esingu-10.4 Inch esigcwele sombala, ukugcizelelwa okukhethekile ekufakweni kwezinto ezithengiswayo ukugwema noma yimuphi umonakalo ngesikhathi sokuthutha, Ukunakwa okuningiliziwe kwekhasimende lethu ngempendulo ezuzisayo.
Siyaqhubeka nomoya wethu webhizinisi "wekhwalithi, ukusebenza, ukusungula izinto ezintsha kanye nobuqotho".Sihlose ukudala inani elithe xaxa kubathengi bethu ngezinsiza zethu ezilayishiwe, imishini yesimanjemanje, abasebenzi abanolwazi kanye nezinsizakalo ezinhle zochwephesheI-China Portabl PCR Thermal Cycler, I-Thermal Cycler emshinini we-PCR, Ngekhwalithi enhle, intengo enengqondo kanye nenkonzo eqotho, sijabulela idumela elihle.Izinto ezithengiswayo zithunyelwa eNingizimu Melika, e-Australia, eNingizimu-mpumalanga ye-Asia njalonjalo.Wamukele ngokufudumele amakhasimende ekhaya nakwamanye amazwe ukuze abambisane nathi ngekusasa eliqhakazile.

Imininingwane

50×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns

Izingxenye zekhithi

Izici&izinzuzo

■ Akukho ukuhlanzwa kwe-DNA okuthatha isikhathi nokubizayo okudingekayo.

■ Isidingo sesampula sincane, njengoba nje kungahlolwa i-fish caudal fin engu-1mg noma amaqanda ayi-10 ezinhlanzi.

■ Azikho izindlela zokwelapha ezikhethekile ezinjengokugaya nokuchoboza okudingekayo, futhi ukuhlinzwa kulula.

■ Uhlelo lwe-PCR olulwa nokungcola 2× PCR EasyTM Mix (UNG), olungaqeda ngempumelelo ukungcola okubangelwa imikhiqizo ye-PCR futhi luqinisekise ukunemba nokunemba kokukhulisa.

■ Uhlelo lwe-PCR olulungiselelwe lenza i-PCR ibe nokucaciswa okuphezulu nokubekezelela okuqinile kuma-PCR reaction inhibitors.

Imingcele yekhithi

Isicelo: Izinhlanzi ze-Zebra nezinye izinhlanzi zamanzi ahlanzekile.

I-DNA ekhishwe kusampula ye-lysis: isetshenziswa kuphela njengesifanekiso se-PCR.

Ikhithi ingasetshenziselwa lezi zinhloso ezilandelayo: ukuhlonza i-transgenosis, i-genotyping yezilwane, njll.

Isitoreji kanye Nempilo yeshelufu

Ingxenye I yale kit kufanele igcinwe ku-2-8℃.

I-reagent Buffer FP ingagcinwa izinyanga eziyi-12 ngaphansi kwezimo ezomile;ingagcinwa ku-2-8℃ ukuze igcinwe isikhathi eside.

v I-Reagent Forgene Protease inefomula eyingqayizivele.Ukuqinisekisa ukusebenza kwayo nokuzinza, sicela uyigcine ku-4℃ izinyanga eziyi-12.

I-vReagent 6×DNA Loading Buffer ingagcinwa ku-4℃ noma -20℃ isikhathi eside.

Ingxenye II yale khithi kufanele igcinwe ku -20 ℃.

I-vReagent 2×PCR EasyTM Mix(UNG), uma isetshenziswa njalo, ingabuye igcinwe ku-4℃ ukuze igcinwe isikhathi esifushane (isetshenziswe phakathi kwezinsuku eziyi-10).Siqhubeka nomoya wethu webhizinisi "wekhwalithi, ukusebenza, ukusungula izinto ezintsha kanye nobuqotho".Sihlose ukudala inani elithe xaxa kubathengi bethu ngezinsiza zethu ezilayishiwe, imishini yesimanjemanje, abasebenzi abanolwazi nezinsizakalo ezinhle zochwepheshe Zamanani Akhethekile e-China Real-Time Quantitative PCR Thermal Cycler enesikrini sokuthinta esingu-10.4 Inch esigcwele sombala, ukugcizelelwa okukhethekile ekufakweni kwezinto ezithengiswayo ukugwema noma yimuphi umonakalo ngesikhathi sokuthutha, Ukunakwa okuningiliziwe kwekhasimende lethu ngempendulo ezuzisayo.
Intengo Ekhethekile yeI-China Portabl PCR Thermal Cycler, I-Thermal Cycler emshinini we-PCR, Ngekhwalithi enhle, intengo enengqondo kanye nenkonzo eqotho, sijabulela idumela elihle.Izinto ezithengiswayo zithunyelwa eNingizimu Melika, e-Australia, eNingizimu-mpumalanga ye-Asia njalonjalo.Wamukele ngokufudumele amakhasimende ekhaya nakwamanye amazwe ukuze abambisane nathi ngekusasa eliqhakazile.