Ikhwalithi ephezulu enokwethenjelwa kanye nokuma okuhle kwesikolodo kuyizimiso zethu, okuzosisiza ukuba sibe sezingeni eliphezulu.Ukunamathela esimisweni esithi "ikhwalithi kuqala, ikhasimende eliphakeme kakhulu" ngentengo Ezwakalayo I-China Hot Selling Good Quality Collection Kit Kits Amasampula Okuqoqwa kwamathe, Sizohlinzeka ngezimpahla zekhwalithi ephezulu nezisombululo ezinhle ngamanani amanani ancintisanayo.Qala ukuzuza ezinsizeni zethu ezibanzi ngokusithinta namuhla.
Ikhwalithi ephezulu enokwethenjelwa kanye nokuma okuhle kwesikolodo kuyizimiso zethu, okuzosisiza ukuba sibe sezingeni eliphezulu.Ukubambelela kumgomo othi "ikhwalithi kuqala, ikhasimende liphezulu" yokuthiIkhithi yokuqoqa amathe yaseChina, Ikhithi Yokuqoqa Amathe Engavamisi, Ukwaneliseka kweKhasimende kuwumgomo wethu.Sibheke ngabomvu ukubambisana nawe futhi sikunikeze izinsizakalo zethu ezingcono kakhulu.Siyakwamukela ngokufudumele ukuthi usithinte futhi sicela ukhululeke ukusithinta.Phequlula igumbi lethu lokubonisa ku-inthanethi ukuze ubone ukuthi singakwenzela ini.Bese usithumela i-imeyili imininingwane yakho noma imibuzo namuhla.

Imininingwane

50 Preps, 200 Preps

Ikhithi isebenzisa ikholomu yokuphotha kanye nefomula eyakhiwe yi-Foregene, engakhipha ngempumelelo i-RNA ephezulu nekhwalithi ephezulu ezicutshini zezitshalo ezihlukahlukene ezinokuqukethwe okuphansi kwama-polysaccharides nama-polyphenols.Kumasampula ezitshalo ane-polysaccharides ephezulu noma okuqukethwe kwama-polyphenols, kunconyelwa ukusebenzisa i-Plant Total RNA Isolation Plus Kit ukuze uthole imiphumela engcono yokukhipha i-RNA.Ikhithi inikeza ikholomu ye-DNA-Cleaning engasusa kalula i-genomic DNA ku-supernatant kanye ne-tissue lysate.Ikholomu ye-RNA kuphela ingabopha ngempumelelo i-RNA.Ikhithi ingacubungula inani elikhulu lamasampuli ngesikhathi esisodwa.

Lonke uhlelo aluqukethe i-RNase, ngakho-ke i-RNA ehlanziwe ngeke yehliswe.I-Buffer PRW1 kanye ne-Buffer PRW2 ingaqinisekisa ukuthi i-RNA etholiwe ayingcoliswanga amaprotheni, i-DNA, ama-ion, nezinhlanganisela eziphilayo.

Izingxenye zekhithi

Izici&izinzuzo

■ Ukusebenza ekamelweni lokushisa (15-25℃) kuyo yonke inqubo, ngaphandle kokugeza eqhweni nokushisa okuphansi kwe-centrifugation.
■ Ikhithi ephelele ye-RNase-Free, asikho isidingo sokukhathazeka ngokuwohloka kwe-RNA.
■ Ikholomu Yokuhlanza I-DNA ibophezela ngokukhethekile ku-DNA, ukuze ikhithi isuse ukungcoliswa kwe-DNA ye-genomic ngaphandle kokwengeza i-DNase.
■ Isivuno esikhulu se-RNA: Ikholomu ye-RNA kuphela kanye nefomula eyingqayizivele ingahlanza i-RNA ngokuphumelelayo.
■ Isivinini esisheshayo: kulula ukusebenza futhi singaqedwa phakathi nemizuzu engama-30.
■ Ukuphepha: asikho i-reagent ephilayo edingekayo.
■ Ikhwalithi ephezulu: Izingcezwana ze-RNA ezihlanziwe zimsulwa kakhulu, azinawo amaphrotheni nokunye ukungcola, futhi zingahlangabezana nezinhlelo zokuhlola ezihlukahlukene ezansi nomfula.

Isicelo sekhithi

Ilungele ukukhishwa nokuhlanzwa kwe-RNA ephelele kumasampula ezicubu zezitshalo ezintsha noma eziqandisiwe (ikakhulukazi izicubu zamaqabunga ezitshalo ezintsha) ezine-polysaccharide ephansi kanye nokuqukethwe kwe-polyphenol.

Ukugeleza komsebenzi

Umdwebo

I-Plant Total RNA Isolation Kit Plus icutshungulwe u-50mg wamaqabunga amasha e-polysaccharides nama-polyphenols, futhi i-RNA ehlanzekile engu-5% yahlolwa nge-electrophoresis.
1: Ubhanana
2: Ginkgo
3: Ukotini
4: Ihalanathi

Isitoreji nempilo yeshelufu

Ikhithi ingagcinwa izinyanga eziyi-12 ekamelweni lokushisa (15-25 ℃) endaweni eyomile, kanye no-2-8 ℃ isikhathi eside (izinyanga ezingu-24).

I-Buffer PSL1 ingagcinwa ku-4 ℃ inyanga engu-1 ngemva kokwengeza i-2-hydroxy-1-ethanethiol(ongakukhetha).Ikhwalithi ephezulu ethembekayo kanye nokuma kwekhredithi enkulu kuyizimiso zethu, ezizosisiza endaweni ephakeme.Ukunamathela esimisweni esithi "ikhwalithi kuqala, ikhasimende eliphakeme kakhulu" ngentengo Ezwakalayo I-China Hot Selling Good Quality Collection Kit Kits Amasampula Okuqoqwa kwamathe, Sizohlinzeka ngezimpahla zekhwalithi ephezulu nezisombululo ezinhle ngamanani amanani ancintisanayo.Qala ukuzuza ezinsizeni zethu ezibanzi ngokusithinta namuhla.
Intengo enengqondoIkhithi yokuqoqa amathe yaseChina, Ikhithi Yokuqoqa Amathe Engavamisi, Ukwaneliseka kweKhasimende kuwumgomo wethu.Sibheke ngabomvu ukubambisana nawe futhi sikunikeze izinsizakalo zethu ezingcono kakhulu.Siyakwamukela ngokufudumele ukuthi usithinte futhi sicela ukhululeke ukusithinta.Phequlula igumbi lethu lokubonisa ku-inthanethi ukuze ubone ukuthi singakwenzela ini.Bese usithumela i-imeyili imininingwane yakho noma imibuzo namuhla.