Iyini i-Direct PCR

I-Direct PCR iyindlela yokukhulisa i-DNA ngqo kusukaigazi, izicubu zezilwane,umsila wegundane,iseli,indlebe yegundane,idube izinhlanzi, amaqanda ezinhlanzi,tshala amaqabunga,imbewunoma elinye isampula lethishu yebhayoloji yoqobo ngaphandle kokwenza ukuhlukaniswa kwe-DNA nezinyathelo zokuyihlanza.Indlela ye-PCR eqondile inganciphisa kakhulu isikhathi sokuhlola, kanye nezindleko kumaphrojekthi we-genotyping kanye nenani eliphezulu.Iphinde inikeze inketho engcono uma ubhekene nezinselele zokukhulisa inani elincane kakhulu lesampula lapho isinyathelo sokuhlanza singase siholele ekulahlekeni kwesampula.

I-ForEGENE's PCR Mix inokumelana nokucindezeleka okuqinile, ngenxa yamandla e-FOREGENE anelungelo lobunikazi be-Taq enzyme( ilungelo lobunikazi eligunyaziwe: ZL20161034512.0 (China), EP3450560 (Europe), PCT/CN2017/082154 (US), i-patent 6894926 (Japan)).I-enzyme yayo ye-Taq isebenzisa ubuchwepheshe bokuhlanganiswa kabusha kwe-DNA ukuze ihlanganise kabusha isifunda sesakhiwo esibophezelayo se-DNA ye-archaeal nucleic acid ebopha iphrotheni ene-Taq DNA polymerase engazweli ukushisa ukuze kwakhiwe isifanekiso se-DNA esinobudlelwane obandayo.I-Taq ethuthukisiwe eshisayo egcina imisebenzi ehlukahlukene ye-DNA polymerase yasekuqaleni.Le enzyme ye-Taq ingabophezela kusifanekiso se-DNA futhi iqalise ukuhlanganiswa kwe-DNA ezifanekisweni zokuhlushwa eziphansi nasezindaweni eziyinkimbinkimbi.

For isibonelo,itkulula njengokungeza kwakhoiseli,isitshalo (iqabunga elincane, impande noma imbewu) noma isampula yesilwanebuffer, futhi wengeza okuhlukilemaster mix withethizeama-primers kumashubhu e-PCR futhi ayisebenzise ngebhayisikili eshisayo.Ukusebenza kahle kwekhithi kulungele ukuhlaziya isampula enkulu lapho isikhathi sokulondoloza sibaluleke kakhulu.

Isistimu yokusabela ye-PCR equkethe i-UNG enzyme ne-dUTP ikhethwa ngokuvumelana nezidingo zokuhlola, ezingagwema ngempumelelo ukungcola okubangelwa imikhiqizo yokukhulisa i-PCR.