Izingcaphuno ezisheshayo nezihle kakhulu, abeluleki abanolwazi ukukusiza ukuthi ukhethe umkhiqizo olungile ovumelana nazo zonke izidingo zakho, isikhathi esifushane sokukhiqiza, ukuphathwa kwekhwalithi ephezulu okunesibopho nezinsizakalo ezihlukile zokukhokha nezokuthumela ngenani elishiwo e-China.Chofoza-It Edu-647Ikhithi Yokutholwa Kweseli, Ibhizinisi lokuqala, siyazwana.Ibhizinisi elengeziwe, ukwethenjwa kuyafika lapho.Ifemu yethu ivamise ukuba senkonzo yakho noma kunini.
Izingcaphuno ezisheshayo nezihle kakhulu, abeluleki abanolwazi ukukusiza ukhethe umkhiqizo olungile ovumelana nazo zonke izidingo zakho, isikhathi esifushane sokukhiqiza, ukuphathwa kwekhwalithi ephezulu okunomthwalo wemfanelo kanye nezinsizakalo ezihlukile zokukhokha kanye nezindaba zokuthumelaIkhithi Yokuthola Amaseli E-China, Chofoza-It Edu-647, Imikhiqizo yethu yaziwa kabanzi futhi yethenjwa ngabasebenzisi futhi ingahlangabezana nokushintsha ngokuqhubekayo kwezidingo zomnotho nezenhlalo.Samukela amakhasimende amasha namadala avela kuyo yonke imikhakha yempilo ukuthi asithinte ukuze uthole ubudlelwano bebhizinisi besikhathi esizayo kanye nempumelelo efanayo!

Kits Izincazelo

50 Preps, 200 Preps

Le kit isebenzisa ikholomu ye-spin kanye nefomula eyakhiwe inkampani yethu, engakwazi ukukhipha i-RNA ephezulu kanye nekhwalithi ephezulu engqikithini yezilwane ezihlukahlukene ngokusebenza kahle okuphezulu.Ihlinzeka ngekholomu ye-DNA-Cleaning ephumelelayo, engakwazi ukuhlukanisa kalula futhi ikhangise i-DNA ye-genomic ku-supernatant kanye ne-tissue lysate, elula futhi elondoloza isikhathi;Ikholomu ye-RNA kuphela ingahlanganisa ngempumelelo i-RNA futhi ingacutshungulwa kanyekanye ngefomula eyingqayizivele Amasampuli amaningi.

Lonke uhlelo luyi-RNase-Free, ukuze i-RNA ekhishiwe ingonakalisiwe;I-Buffer RW1, i-Buffer RW2 isistimu yokugeza ibhafa, ukuze i-RNA etholiwe ingabi nawo amaprotheni, i-DNA, i-ion, kanye nokungcoliswa kwenhlanganisela yezinto eziphilayo.

Izingxenye zekhithi:

Izici&izinzuzo

■ Asikho isidingo sokukhathazeka ngokuwohloka kwe-RNA;lonke uhlelo luyi-RNase-Free
■ Khipha ngempumelelo i-DNA usebenzisa i-DNA-Cleaning Column
■ Khipha i-DNA ngaphandle kokwengeza i-DNase
■ Kulula-yonke imisebenzi iqedwa ekamelweni lokushisa
■ Ukusebenza okusheshayo kungaqedwa ngemizuzu engama-30
■ I-Safe-akukho reagent ephilayo edingekayo
■ Ukuhlanzeka okuphezulu -OD260/280≈1.8-2.1

Isicelo sekhithi

Ilungele ukukhishwa nokuhlanzwa kwe-RNA ephelele ezinhlobonhlobo zezicubu zezilwane ezintsha noma eziqandisiwe noma amaseli akhulisiwe.

Imingcele yomkhiqizo

■ Izinhlelo zokusebenza eziwela phansi: ukuhlanganiswa kwe-cDNA ye-first-strand, i-RT-PCR, i-molecular cloning, i-Northern Blot, njll.
■ Amasampula: izicubu zezilwane, amaseli akhulisiwe
■ Umthamo: Izicubu 10-20mg, Amaseli(2-5)×10⁶
■ Umthamo omkhulu wokubopha we-DNA wekholomu yokuhlanza: 80 μg
■ Ivolumu ye-Elution: 50-200 μl

Ukugeleza komsebenzi

Umdwebo

I-Animal Total RNA Isolation Kit iphathwe ama-20mg
Amasampula egundane amasha, thatha i-RNA ephelele engu-5% 1% ye-agar

I-Glycogel electrophoresis
1: Ubende 2: Izinso
3: Isibindi 4: Inhliziyo

Isitoreji nempilo yeshelufu

Ikhithi ingagcinwa izinyanga ezingama-24 ekamelweni lokushisa (15-25 ℃) noma 2–8 ℃ isikhathi eside.I-Buffer RL1 ingagcinwa ngo-4 ℃ ngenyanga eyi-1 ngemuva kokungeza i-β- reecatothanatol (ngokuzithandela) Ikati elifanelekile le-China China-I-Edu-647 Cell Preficeration Detection Detection, Interprise yokuqala, siyaqondana.Ibhizinisi elengeziwe, ukwethenjwa kuyafika lapho.Ifemu yethu ivamise ukuba senkonzo yakho noma kunini.
Intengo ecashuniwe yeIkhithi Yokuthola Amaseli E-China, Click-It Edu-647, Imikhiqizo yethu yaziwa kabanzi futhi yethenjwa ngabasebenzisi futhi ingahlangabezana nokushintsha ngokuqhubekayo kwezidingo zomnotho nezenhlalo.Samukela amakhasimende amasha namadala avela kuyo yonke imikhakha yempilo ukuthi asithinte ukuze uthole ubudlelwano bebhizinisi besikhathi esizayo kanye nempumelelo efanayo!