"Lawula izinga ngemininingwane, bonisa amandla ngekhwalithi".Ifemu yethu izamile ukusungula iqembu labasebenzi elisebenza kahle kakhulu futhi elizinzile futhi yahlola indlela esebenza kahle kakhulu yokuyala ye-Original Factory China High Sensitivity HS Hotstart Taq DNA Polymerase ene-Loading Buffer kanye ne-dNTP PCR Kit, Samukela amakhasimende amasha nangaphambili avela kuyo yonke imikhakha yokuphila kwansuku zonke ukuze asibambe ukuze asibambe ukuze sithole izinzuzo zebhizinisi esikhathini esizayo kanye nokusebenzelana kwebhizinisi okuzayo.
"Lawula izinga ngemininingwane, bonisa amandla ngekhwalithi".Inkampani yethu izamile ukusungula ithimba labasebenzi elisebenza kahle kakhulu futhi elizinzile futhi yahlola indlela esebenza kahle yokuyalaI-China PCR Amplification, Qpcr, Inkampani yethu inamathela emoyeni "wezindleko eziphansi, ikhwalithi ephezulu, nokwenza izinzuzo ezengeziwe kumakhasimende ethu".Ukusebenzisa amathalenta avela kulayini ofanayo nokunamathela esimisweni esithi "ukwethembeka, ukholo oluhle, into yangempela nobuqotho", inkampani yethu inethemba lokuzuza ukuthuthukiswa okufanayo namakhasimende avela ekhaya nakwamanye amazwe!

Imininingwane

50 Preps, 200 Preps

Ikhithi isebenzisa ikholomu yokuphotha kanye nefomula eyakhiwe yi-Foregene, engakhipha ngempumelelo i-RNA ephezulu nekhwalithi ephezulu ezicutshini zezitshalo ezihlukahlukene ezinokuqukethwe okuphansi kwama-polysaccharides nama-polyphenols.Kumasampula ezitshalo ane-polysaccharides ephezulu noma okuqukethwe kwama-polyphenols, kunconyelwa ukusebenzisa i-Plant Total RNA Isolation Plus Kit ukuze uthole imiphumela engcono yokukhipha i-RNA.Ikhithi inikeza ikholomu ye-DNA-Cleaning engasusa kalula i-genomic DNA ku-supernatant kanye ne-tissue lysate.Ikholomu ye-RNA kuphela ingabopha ngempumelelo i-RNA.Ikhithi ingacubungula inani elikhulu lamasampuli ngesikhathi esisodwa.

Lonke uhlelo aluqukethe i-RNase, ngakho-ke i-RNA ehlanziwe ngeke yehliswe.I-Buffer PRW1 kanye ne-Buffer PRW2 ingaqinisekisa ukuthi i-RNA etholiwe ayingcoliswanga amaprotheni, i-DNA, ama-ion, nezinhlanganisela eziphilayo.

Izingxenye zekhithi

Izici&izinzuzo

■ Ukusebenza ekamelweni lokushisa (15-25℃) kuyo yonke inqubo, ngaphandle kokugeza eqhweni nokushisa okuphansi kwe-centrifugation.
■ Ikhithi ephelele ye-RNase-Free, asikho isidingo sokukhathazeka ngokuwohloka kwe-RNA.
■ Ikholomu Yokuhlanza I-DNA ibophezela ngokukhethekile ku-DNA, ukuze ikhithi isuse ukungcoliswa kwe-DNA ye-genomic ngaphandle kokwengeza i-DNase.
■ Isivuno esikhulu se-RNA: Ikholomu ye-RNA kuphela kanye nefomula eyingqayizivele ingahlanza i-RNA ngokuphumelelayo.
■ Isivinini esisheshayo: kulula ukusebenza futhi singaqedwa phakathi nemizuzu engama-30.
■ Ukuphepha: asikho i-reagent ephilayo edingekayo.
■ Ikhwalithi ephezulu: Izingcezwana ze-RNA ezihlanziwe zimsulwa kakhulu, azinawo amaphrotheni nokunye ukungcola, futhi zingahlangabezana nezinhlelo zokuhlola ezihlukahlukene ezansi nomfula.

Isicelo sekhithi

Ilungele ukukhishwa nokuhlanzwa kwe-RNA ephelele kumasampula ezicubu zezitshalo ezintsha noma eziqandisiwe (ikakhulukazi izicubu zamaqabunga ezitshalo ezintsha) ezine-polysaccharide ephansi kanye nokuqukethwe kwe-polyphenol.

Ukugeleza komsebenzi

Umdwebo

I-Plant Total RNA Isolation Kit Plus icutshungulwe u-50mg wamaqabunga amasha e-polysaccharides nama-polyphenols, futhi i-RNA ehlanzekile engu-5% yahlolwa nge-electrophoresis.
1: Ubhanana
2: Ginkgo
3: Ukotini
4: Ihalanathi

Isitoreji nempilo yeshelufu

Ikhithi ingagcinwa izinyanga eziyi-12 ekamelweni lokushisa (15-25 ℃) endaweni eyomile, kanye no-2-8 ℃ isikhathi eside (izinyanga ezingu-24).

I-Buffer PSL1 ingagcinwa kokuthi 4 ℃ inyanga engu-1 ngemva kokwengeza i-2-hydroxy-1-ethanethiol(ongakukhetha).”Lawula izinga ngemininingwane, bonisa amandla ngekhwalithi”.Ifemu yethu izamile ukusungula iqembu labasebenzi elisebenza kahle kakhulu futhi elizinzile futhi yahlola indlela esebenza kahle kakhulu yokuyala ye-Original Factory China High Sensitivity HS Hotstart Taq DNA Polymerase ene-Loading Buffer kanye ne-dNTP PCR Kit, Samukela amakhasimende amasha nangaphambili avela kuyo yonke imikhakha yokuphila kwansuku zonke ukuze asibambe ukuze asibambe ukuze sithole izinzuzo zebhizinisi esikhathini esizayo kanye nokusebenzelana kwebhizinisi okuzayo.
Imboni YoqoboI-China PCR Amplification, Qpcr, Inkampani yethu inamathela emoyeni "wezindleko eziphansi, ikhwalithi ephezulu, nokwenza izinzuzo ezengeziwe kumakhasimende ethu".Ukusebenzisa amathalenta avela kulayini ofanayo nokunamathela esimisweni esithi "ukwethembeka, ukholo oluhle, into yangempela nobuqotho", inkampani yethu inethemba lokuzuza ukuthuthukiswa okufanayo namakhasimende avela ekhaya nakwamanye amazwe!