Sihlala sinamathela kumgomo othi “Ikhwalithi Yokuqala, Udumo Oluphakeme”.Sizibophezele ngokugcwele ekuhlinzekeni amaklayenti ethu ngemikhiqizo yekhwalithi enenani elincintisanayo, ukulethwa okusheshayo kanye nenkonzo yobungcweti Yesaphulelo Esijwayelekile.I-China Nucleic Acid Extraction Reagent(Isixazululo se-Serum/ Plasma ne-Puncture), Amakhithi Wokuhlola I-Rna Extraction, Sigxila ekwakhiweni komkhiqizo wethu futhi sihambisana nezinkulumo eziningi ezinokuhlangenwe nakho kanye nemishini yezinga lokuqala.Izimpahla zethu okufanele ube nazo.
Sihlala sinamathela kumgomo othi “Ikhwalithi Yokuqala, Udumo Oluphakeme”.Sizibophezele ngokugcwele ekuhlinzekeni amaklayenti ethu ngemikhiqizo yekhwalithi enenani elincintisanayo, ukulethwa okusheshayo kanye nenkonzo yobungcwetiI-China Nucleic Acid Extraction Reagent, Indlela ye-Magnetic Bead, Inqubomgomo Yenkampani yethu “iyikhwalithi kuqala, ukuba ngcono futhi ibe namandla, intuthuko eqhubekayo” .Izinjongo zethu “zokuba umphakathi, amakhasimende, abasebenzi, ozakwethu kanye namabhizinisi bafune inzuzo ephusile”.Sifisa ukubambisana nabo bonke abakhiqizi bezingxenye zezimoto abahlukene, isitolo sokulungisa izimoto, intanga yezimoto, bese sidala ikusasa elihle!Siyabonga ngokuzinika isikhathi sokubheka iwebhusayithi yethu futhi singakwamukela noma yiziphi iziphakamiso ongase ube nazo ezingasisiza ukuba sithuthukise isayithi lethu.

Kits Izincazelo

50 Preps, 200 Preps

Le kit isebenzisa ikholomu ye-spin kanye nefomula eyakhiwe inkampani yethu, engakwazi ukukhipha i-RNA ephezulu kanye nekhwalithi ephezulu engqikithini yezilwane ezihlukahlukene ngokusebenza kahle okuphezulu.Ihlinzeka ngekholomu ye-DNA-Cleaning ephumelelayo, engakwazi ukuhlukanisa kalula futhi ikhangise i-DNA ye-genomic ku-supernatant kanye ne-tissue lysate, elula futhi elondoloza isikhathi;Ikholomu ye-RNA kuphela ingahlanganisa ngempumelelo i-RNA futhi ingacutshungulwa kanyekanye ngefomula eyingqayizivele Amasampuli amaningi.

Lonke uhlelo luyi-RNase-Free, ukuze i-RNA ekhishiwe ingonakalisiwe;I-Buffer RW1, i-Buffer RW2 isistimu yokugeza ibhafa, ukuze i-RNA etholiwe ingabi nawo amaprotheni, i-DNA, i-ion, kanye nokungcoliswa kwenhlanganisela yezinto eziphilayo.

Izingxenye zekhithi:

Izici&izinzuzo

■ Asikho isidingo sokukhathazeka ngokuwohloka kwe-RNA;lonke uhlelo luyi-RNase-Free
■ Khipha ngempumelelo i-DNA usebenzisa i-DNA-Cleaning Column
■ Khipha i-DNA ngaphandle kokwengeza i-DNase
■ Kulula-yonke imisebenzi iqedwa ekamelweni lokushisa
■ Ukusebenza okusheshayo kungaqedwa ngemizuzu engama-30
■ I-Safe-akukho reagent ephilayo edingekayo
■ Ukuhlanzeka okuphezulu -OD260/280≈1.8-2.1

Isicelo sekhithi

Ilungele ukukhishwa nokuhlanzwa kwe-RNA ephelele ezinhlobonhlobo zezicubu zezilwane ezintsha noma eziqandisiwe noma amaseli akhulisiwe.

Imingcele yomkhiqizo

■ Izinhlelo zokusebenza eziwela phansi: ukuhlanganiswa kwe-cDNA ye-first-strand, i-RT-PCR, i-molecular cloning, i-Northern Blot, njll.
■ Amasampula: izicubu zezilwane, amaseli akhulisiwe
■ Umthamo: Izicubu 10-20mg, Amaseli(2-5)×10⁶
■ Umthamo omkhulu wokubopha we-DNA wekholomu yokuhlanza: 80 μg
■ Ivolumu ye-Elution: 50-200 μl

Ukugeleza komsebenzi

Umdwebo

I-Animal Total RNA Isolation Kit iphathwe ama-20mg
Amasampula egundane amasha, thatha i-RNA ephelele engu-5% 1% ye-agar

I-Glycogel electrophoresis
1: Ubende 2: Izinso
3: Isibindi 4: Inhliziyo

Isitoreji nempilo yeshelufu

Ikhithi ingagcinwa izinyanga ezingama-24 ekamelweni lokushisa (15-25 ℃) noma 2–8 ℃ isikhathi eside.I-Buffer RL1 ingagcinwa ku-4 ℃ inyanga engu-1 ngemva kokwengeza i-β- mercaptoethanol (uma uthanda).

Sihlala sinamathela kumgomo othi “Ikhwalithi Yokuqala, Udumo Oluphakeme”.Sizibophezele ngokugcwele ekuhlinzekeni amaklayenti ethu ngemikhiqizo yekhwalithi enenani elincintisanayo, ukulethwa okusheshayo kanye nenkonzo yobungcweti Yesaphulelo Esivamile sase-China Nucleic Acid Extraction Reagent (Isixazululo se-Serum/ Plasma ne-Puncture), I-Rna Extraction Test Kits, Sigxila ekwakhiweni komkhiqizo wethu futhi sihambisana nezinkulumo eziningi ezinolwazi kanye nemishini yezinga lokuqala.Izimpahla zethu okufanele ube nazo.
Isaphulelo Esivamile sase-China Nucleic Acid Extraction Reagent,Indlela ye-Magnetic Bead, Inqubomgomo Yenkampani yethu “iyikhwalithi kuqala, ukuba ngcono futhi ibe namandla, intuthuko eqhubekayo” .Izinjongo zethu “zokuba umphakathi, amakhasimende, abasebenzi, ozakwethu kanye namabhizinisi bafune inzuzo ephusile”.Sifisa ukubambisana nabo bonke abakhiqizi bezingxenye zezimoto abahlukene, isitolo sokulungisa izimoto, intanga yezimoto, bese sidala ikusasa elihle!Siyabonga ngokuzinika isikhathi sokubheka iwebhusayithi yethu futhi singakwamukela noma yiziphi iziphakamiso ongase ube nazo ezingasisiza ukuba sithuthukise isayithi lethu.