I-China OEM/ODM Factory China Magnetic Beads Method Nucleic Acid Rna Extraction Reagent Kits Yesikhathi Sangempela Umkhiqizi Nomphakeli Wokutholwa kwe-PCR |I-Foregene

I-OEM/ODM Factory China Magnetic Beads Method Nucleic Acid Rna Extraction Reagent Kits Yesikhathi Sangempela Ukutholwa kwe-PCR

Incazelo yekhithi:

Le kit isebenzisa isistimu ye-lysis buffer eyingqayizivele ukuze ikhulule ngokushesha i-DNA ye-genomic emisileni yegundane, ezindlebeni, emisipha kanye namanye amasampula ezicubu zokusabela kwe-PCR, ngakho ifaneleka ngokukhethekile ukuhlolwa kofuzo okukhulu.

Inqubo yokukhipha i-DNA ye-genomic ku-lysis buffer iqedwa phakathi kwemizuzu eyi-10-30 ku-65°C.Azikho ezinye izinqubo ezidingekayo njengokususwa kwamaprotheni kanye ne-RNA, futhi i-trace DNA ekhishiwe ingasetshenziswa njengesifanekiso sokusabela kwe-PCR.

I-2× M1-PCR EasyTM Mix (UNG) isebenzisa i-dUTP esikhundleni se-dTTP ngesisekelo se-2× M1-PCR EasyTM Mix (UNG), futhi yengeza i-UNG enzyme (Uracil-N-glycosylase) engalulaza isifanekiso esiqukethe i-dUTP ngesikhathi esifanayo.Ngaphambi kokusabela kwe-PCR, i-enzyme ye-UNG isetshenziselwa ukuthunaza umkhiqizo we-PCR oqukethe i-uracil.I-enzyme ye-UNG ngeke ibe namuphi umthelela kusifanekiso esingaqukethe i-uracil, ngaleyo ndlela iqinisekise ukucaciswa nokunemba kokukhulisa nokuvimbela amathuba okuhlolwa kofuzo okukhulu.Ukungcoliswa kwemikhiqizo ye-PCR kwenzekile.

I-D-Taq DNA polymerase iyi-polymerase ye-DNA ethuthukiswe ngokukhethekile yi-Foregene ukuze kutholakale ukusabela okuqondile kwe-PCR.I-D-Taq DNA polymerase inokubekezelela okunamandla ezinhlobonhlobo ze-PCR reaction inhibitors, futhi ingakhulisa kahle amanani omkhondo we-DNA ezinhlelweni ezihlukene zokusabela eziyinkimbinkimbi, futhi isivinini sokukhulisa singafinyelela ku-2Kb/min, okulungele ikakhulukazi ukusabela kwe-Direct PCR.

Thumela i-imeyili kithi

Imininingwane Yomkhiqizo

Omaka bomkhiqizo

Ibhizinisi lethu libambelele kumgomo oyisisekelo othi "Ikhwalithi ingaba impilo nenkampani, futhi irekhodi elizoba umphefumulo wayo" ku-OEM/ODM Factory China Magnetic Beads Method Nucleic Acid Rna Extraction Reagent Kits Yokutholwa Kwesikhathi Sangempela I-PCR, Abasebenzi bethu banomgomo wokuhlinzeka ngezixazululo ngesilinganiso esiphezulu sezindleko zokusebenza kumathemba ethu, futhi kuhlale kuqondiswe kubathengi bethu yonke indawo.
Ibhizinisi lethu libambelele kumgomo oyisisekelo othi "Ikhwalithi ingaba impilo yenkampani, futhi irekhodi elizoba umphefumulo wayo"Ukuhlanzwa okuzenzakalelayo kwe-Nucleic Acid, I-China Universal Rna DNA Reagents, Namuhla imikhiqizo yethu ithengiswa kuwo wonke amazwe asekhaya nakwamanye amazwe ngenxa yokusekelwa kwamakhasimende avamile namasha.Sethula umkhiqizo wekhwalithi ephezulu kanye nentengo yokuncintisana, wamukela amakhasimende avamile namasha abambisana nathi!

Imininingwane

50×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns

Izingxenye zekhithi

Ingxenye I	Buffer MP
	I-Foregene Protease Plus
	I-6× I-DNA Yokulayisha Ibhafa
Ingxenye II	2× M1 PCR Kulula^TMImiksi(UNG)
Iziyalezo

Izici&izinzuzo

■ Akukho ukuhlanzwa kwe-DNA okuthatha isikhathi nokubizayo okudingekayo.

■ Isidingo sesampula sincane, njengoba nje kungahlolwa izicubu zegundane eziyi-5mg noma umsila wegundane ongu-2-5mm.

■ Azikho izindlela zokwelapha ezikhethekile ezinjengokugaya nokuchoboza okudingekayo, futhi ukuhlinzwa kulula.

■ Isistimu ye-PCR ethuthukisiwe yenza i-PCR ibe nokucaciswa okuphezulu nokubekezelela okuqinile kuma-PCR reaction inhibitors.

■ Isistimu ye-PCR yokunqanda ukungcoliswa 2×M1-PCR Kulula^TMI-Mix (UNG), eqeda ngempumelelo ukungcola okubangelwa imikhiqizo ye-PCR futhi iqinisekise ukucaciswa nokunemba kokukhulisa.

Imingcele yekhithi

Ububanzi bokusebenza: umsila wegundane, indlebe yegundane, imisipha nezinye izicubu.

I-DNA ekhishwe kusampula ye-lysis: isetshenziswa kuphela njengesifanekiso se-PCR.

Ikhithi ingasetshenziselwa lezi zinhloso ezilandelayo: ukuhlonza i-transgenosis, i-genotyping yezilwane, njll.

Ukugeleza komsebenzi

wk flw-mouse umsila oqondile PCR

Isitoreji kanye Nempilo yeshelufu

Ingxenye I yale kit kufanele igcinwe ku-2-8℃.

I-reagent Buffer AL ingagcinwa izinyanga eziyi-12 ngaphansi kwezimo ezomile;ingagcinwa ku-2-8℃ ukuze igcinwe isikhathi eside.

I-Reagent Foregene Protease inefomula eyingqayizivele.Ukuqinisekisa ukusebenza kwayo nokuzinza, sicela uyigcine ku-4℃ izinyanga eziyi-12.

I-Reagent 6×DNA Loading Buffer ingagcinwa ku-4℃ noma -20℃ isikhathi eside.

Ingxenye II yale khithi kufanele igcinwe ku -20 ℃.

I-Reagent 2×M1 PCR EasyTM Mix(UNG), uma isetshenziswa njalo, ingabuye igcinwe ku-4℃ ukuze igcinwe isikhathi esifushane (isetshenziswe phakathi nezinsuku ezingu-10).Ibhizinisi lethu linamathela kumgomo oyisisekelo othi “Ikhwalithi ingaba ukuphila nenkampani, futhi irekhodi liyoba umphefumulo wayo” ku-OEM/ODM Factory China Magnetic Beads PC Realtraction Act Real staff Reid Ration Delectr umgomo wokuhlinzeka ngezixazululo ezinesilinganiso esiphezulu sezindleko zokusebenza kumathemba ethu, kanye nethagethi yethu sonke iwukuba njalo ukwanelisa abathengi bethu abavela yonke indawo emhlabeni.
I-OEM/ODM FactoryI-China Universal Rna DNA Reagents, Ukuhlanzwa okuzenzakalelayo kwe-Nucleic Acid, Namuhla imikhiqizo yethu ithengiswa kuwo wonke amazwe asekhaya nakwamanye amazwe ngenxa yokusekelwa kwamakhasimende avamile namasha.Sethula umkhiqizo wekhwalithi ephezulu kanye nentengo yokuncintisana, wamukela amakhasimende avamile namasha abambisana nathi!

Okwedlule: I-ODM Factory China Mtt Detection Kit 1000t Isisetshenziswa Sokuthola Ukusebenza Kweseli

Olandelayo: I-OEM/ODM Factory China dNTP MIX (10mM)

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