I-OEM/ODM China Viral DNA&Rna Nucleic Acid Extraction Kit Yesikhathi Sangempela PCR
Ukuphishekela kwethu kanye nenjongo yenkampani iwukuba njalo “ukwanelisa njalo izimfuneko zethu zabathengi”.Siyaqhubeka nokuthola nesitayela futhi siklama imikhiqizo yeqophelo eliphezulu kumakhasimende ethu aphelelwe yisikhathi namasha futhi sifinyelela ithemba lokuwina kubathengi bethu kanye nathi nge-OEM/ODM China Viral DNA&Rna Nucleic Acid Extraction Kit Yesikhathi Sangempela PCR, Siphikelela ukuthola umoya wethu webhizinisi “ikhwalithi yokuphila kwenhlangano, isikweletu siqinisekisa ukubambisana ngaphakathi kwethu futhi siqhubeke sikhumbula isiqubulo sethu kuqala.
Ukuphishekela kwethu kanye nenjongo yenkampani iwukuba njalo “ukwanelisa njalo izimfuneko zethu zabathengi”.Siyaqhubeka nokuthola nesitayela nokuklama imikhiqizo yekhwalithi ephezulu kumakhasimende ethu aphelelwe yisikhathi namasha futhi sifinyelele ithemba lokuwina kubathengi bethu kanye nathiI-China Viral Rna/DNA Extraction Kit kanye ne-Magnetic Bead Rna&DNA Extraction Kit, Sithole i-ISO9001 enikeza isisekelo esiqinile sokuthuthuka kwethu okwengeziwe.Ukuphikelela kokuthi “Ikhwalithi ephezulu, Ukulethwa Ngokushesha, Intengo Encintisanayo”, sisungule ukubambisana kwesikhathi eside namakhasimende avela phesheya kwezilwandle nasekhaya futhi sithole ukuphawula okuphezulu kwamaklayenti amasha namadala.Kuyilungelo lethu elikhulu ukuhlangabezana nezidingo zakho.Silindele ukunaka kwakho.
Amamanuwali wemiyalo:
Ukuphishekela kwethu kanye nenjongo yenkampani iwukuba njalo “ukwanelisa njalo izimfuneko zethu zabathengi”.Siyaqhubeka nokuthola nesitayela futhi siklama imikhiqizo yeqophelo eliphezulu kumakhasimende ethu aphelelwe yisikhathi namasha futhi sifinyelela ithemba lokuwina kubathengi bethu kanye nathi nge-OEM/ODM China Viral DNA&Rna Nucleic Acid Extraction Kit Yesikhathi Sangempela PCR, Siphikelela ukuthola umoya wethu webhizinisi “ikhwalithi yokuphila kwenhlangano, isikweletu siqinisekisa ukubambisana ngaphakathi kwethu futhi siqhubeke sikhumbula isiqubulo sethu kuqala.
I-OEM/ODM China China Viral Rna/DNA Extraction Kit kanye ne-Magnetic Bead Rna&DNA Extraction Kit, Sithole i-ISO9001 enikeza isisekelo esiqinile sokuthuthuka kwethu okuqhubekayo.Ukuphikelela kokuthi “Ikhwalithi ephezulu, Ukulethwa Ngokushesha, Intengo Encintisanayo”, sisungule ukubambisana kwesikhathi eside namakhasimende avela phesheya kwezilwandle nasekhaya futhi sithole ukuphawula okuphezulu kwamaklayenti amasha namadala.Kuyilungelo lethu elikhulu ukuhlangabezana nezidingo zakho.Silindele ukunaka kwakho.
Umhlahlandlela Wokuhlaziya Inkinga
Okulandelayo ukuhlaziya izinkinga ezingase kuhlangatshezwane nazo ekukhishweni kwe-viral DNA/RNA, ngethemba lokuba usizo ekuhloleni kwakho.Ngaphezu kwalokho, kwezinye izinkinga zokuhlola noma zobuchwepheshe ngaphandle kwemiyalo yokusebenza nokuhlaziya inkinga, sinikele ngosekelo lobuchwepheshe ukuze sikusize.Uma unezinye izidingo, sicela usithinte: 028-83360257 noma E-mail:
Tech@foregene.com.
Akukho ukukhishwa kwe-nucleic acid noma isivuno esiphansi se-nucleic acid
Ngokuvamile kuba nezinto eziningi ezithinta ukusebenza kahle kokululama, njengalezi: isampula yokuqukethwe kwe-nucleic acid, indlela yokusebenza, ivolumu ye-elution, njll.
Ukuhlaziywa kwezimbangela ezijwayelekile:
1. Ukugeza kweqhwa noma izinga lokushisa eliphansi (4 ° C) i-centrifugation yenziwa ngesikhathi senqubo.
Ukusikisela: Sebenzisa ekamelweni lokushisa (15-25°C) kuyo yonke inqubo, ungabhavi eqhweni kanye nokushisa okuphansi kwe-centrifugation.
2. Isampula ligcinwe ngendlela engafanele noma isampula ligcinwe isikhathi eside kakhulu.
Okutuswayo: Gcina amasampula ku -80°C futhi ugweme ukuqhwa okuphindaphindiwe nokuncibilika;zama ukusebenzisa amasampula asanda kuqoqwa ukuze kukhishwe i-nucleic acid.
3. Isampula yesampula enganele.
Okutuswayo: Sicela uqinisekise ukuthi isampula kanye nesisombululo sokusebenza se-lysis kuxutshwe kahle futhi kufakwe endaweni yokushisa yegumbi (15-25°C) imizuzu eyi-10.
4. Ukwengezwa okungalungile kwe-eluent.
Isiphakamiso: Qiniseka ukuthi i-ddH2O Engenayo i-RNase yengezwa ngokudonsela phansi maphakathi nolwelwesi lwekholomu yokuhlanza, futhi ungayibeki eringini yekholomu yokuhlanza.
5. Ivolumu elungile ye-ethanol ephelele ayizange yengezwe ku-Buffer RW2.
Ukusikisela: Sicela ulandele imiyalelo, wengeze ivolumu efanele ye-ethanol ephelele ku-Buffer RW2 futhi uhlanganise kahle ngaphambi kokusebenzisa ikhithi.
6. Ivolumu yesampula engafanele.
Isiphakamiso: U-200µl wesampula ucutshungulwa kuwo wonke u-500µl we-Buffer DRL.Ukucutshungulwa kwesampula okweqile kuzoholela emvuzweni ephansi yokukhipha i-nucleic acid.
7. Ivolumu ye-elution engafanele noma ukungezwani okuphelele.
Isincomo: Ivolumu ecacile yekholomu yokuhlanza ingu-30-50μl;uma umphumela we-elution ungagculisi, kuyanconywa ukuthi unwebe isikhathi kuzinga lokushisa legumbi ngemva kokwengeza i-ddH2O eshisiwe ye-RNase-Free, njenge-5-10min.
8. I-Ethanol ihlala kukholamu ngemva kokugeza nge-Buffer RW2.
Isiphakamiso: Uma i-ethanol isala ngemva kokufakwa phakathi kwe-centrifugation ne-Buffer RW2 imizuzu emi-2, ikholomu ingabekwa ekamelweni lokushisa imizuzu emi-5 ngemva kwe-centrifugation ukuze ikhiphe ngokuphelele i-ethanol esele.
I-nucleic acid ehlanzekile iyancipha
Izinga le-nucleic acid ehlanzekile lihlobene nokulondolozwa kwesampula, ukungcoliswa kwe-RNase, ukusebenza nezinye izici.Ukuhlaziywa kwezimbangela ezijwayelekile:
1. Amasampula aqoqiwe awagcinwanga ngesikhathi.
Ukusikisela: Uma isampuli ingasetshenziswa ngesikhathi ngemva kokuqoqwa, sicela uyigcine ku -80°C ezingeni lokushisa eliphansi ngokushesha.Ukuze uthole i-RNA, zama ukusebenzisa amasampuli asanda kuqoqwa.
2. Qoqa amasampula bese uwaqandisa futhi uncibilike ngokuphindaphindiwe.
Ukusikisela: Gwema ukuqanda nokuncibilika (hhayi ngaphezu kokukodwa) ngesikhathi sokuqoqwa nokugcinwa kwamasampula, ngaphandle kwalokho isivuno se-nucleic acid sizoncipha.
3. I-RNase yethulwa egumbini lokuhlinza noma amagilavu alahlwayo, imaski, njll. awagqokwa.
Okutuswayo: Ukuhlolwa kokukhipha i-RNA kwenziwa kangcono kakhulu ekamelweni lokusebenza elihlukile le-RNA, futhi itafula laselabhorethri kufanele lihlanzwe ngaphambi kokuhlolwa.
Gqoka amagilavu alahlwayo namamaski ngesikhathi sokuhlolwa ukuze ugweme ukucekelwa phansi kwe-RNA okubangelwa ukwethulwa kwe-RNase ngezinga elikhulu kakhulu.
4. I-reagent yonakaliswe i-RNase ngesikhathi sokusetshenziswa.
Isincomo: Faka esikhundleni se-Viral DNA/RNA Isolation Kit entsha ukuze uthole ukuhlolwa okuhlobene.
5. Amashubhu e-centrifuge namathiphu e-piette asetshenziselwa ukukhwabanisa kwe-RNA angcoliswe yi-RNase.
Ukusikisela: Qinisekisa ukuthi amashubhu e-centrifuge, amathiphu e-pipette, amapayipi, njll. asetshenziselwa ukukhipha i-RNA konke Akuna-RNAse.
I-nucleic acid ehlanjululwe ithinta ukuhlolwa okungezansi komfula
I-DNA ne-RNA ehlanjululwe yikholomu yokuhlanza, uma i-ion kasawoti kanye nokuqukethwe kwamaprotheni kuphezulu kakhulu, kuzothinta ukuhlolwa okuya phansi komfula, okufana nalokhu: Ukukhulisa i-PCR, ukuloba okuhlanekezelwe, njll.
1. I-DNA ekhishiwe kanye ne-RNA inama-ion kasawoti asele.
Isiphakamiso: Qinisekisa ukuthi ivolumu elungile ye-ethanol ephelele yengezwa ku-Buffer RW2, futhi ugeze ikholomu yokuhlanza kabili ngesivinini sokuhlanganisa esishiwo emiyalweni yokusebenza;Yenza i-centrifugation ukuze unciphise ukungcoliswa kwe-ion kasawoti.
2. I-DNA ekhishiwe kanye ne-RNA inezinsalela ze-ethanol.
Ukusikisela: Ngemva kokuqinisekisa ukugeza nge-Buffer RW2, yenza i-tube centrifugation engenalutho ngesivinini se-centrifugation emiyalweni yokusebenza;uma kusekhona izinsalela ze-ethanol, ungakwazi ukufaka i-centrifuge ishubhu elingenalutho bese ulibeka endaweni yokushisa yasekamelweni imizuzu engu-5 ukuze ususe insalela ye-ethanol ngezinga elikhulu kakhulu.
Amamanuwali wemiyalo:
I-Viral DNA&RNA Isolation Kit Instruction Manual