Enesimo sengqondo esihle nesinenqubekelaphambili ekuthakaseleni kwamakhasimende, ibhizinisi lethu lihlala lithuthukisa into yethu ngendlela enhle kakhulu ukwanelisa izifiso zamakhasimende futhi ligxile kakhulu kwezokuphepha, ukwethembeka, izimfuno zemvelo, kanye nokuqanjwa okusha Komkhiqizi we-China Magnetic Beads Nucleic Acid PCR Extraction Kit ene-CE yomshini we-Poct PCR, Ngokuzithoba sikhula ndawonye nemvelo yethu.
Enesimo sengqondo esihle nesinenqubekela phambili ekuthatheni kwakhe amakhasimende, ibhizinisi lethu lihlala lithuthukisa into yethu ngendlela enhle kakhulu ukuze lanelise izifiso zamakhasimende futhi ligxile kakhulu ekuphepheni, ekuthembekeni, ekufuneni imvelo, nasekusunguleni izinto ezintsha.Ikhithi yokukhipha iDNA yaseChina, Ikhithi yokukhipha i-Nucleic Acid, Sinamathela kuklayenti 1st, izinga eliphezulu 1st, ukuthuthukiswa okuqhubekayo, ukusizakala kanye nemigomo yokuwina-win.Uma sibambisana nekhasimende, siletha abathengi abanekhwalithi ephezulu kakhulu yesevisi.Sisungule ubudlelwano obuhle bebhizinisi sisebenzisa umthengi waseZimbabwe ngaphakathi kwebhizinisi, sesizakhele igama nesithunzi.Ngesikhathi esifanayo, ngenhliziyo yonke wamukele amathemba amasha namadala enkampanini yethu ukuya futhi axoxisane namabhizinisi amancane.

Izincazelo

Le khithi isebenzisa isistimu ye-lysis buffer eyingqayizivele engakhulula ngokushesha i-RNA kumasampula eseli akhulisiwe ukuze kusabela i-RT-qPCR, ngaleyo ndlela iqede inqubo yokuhlanza i-RNA edla isikhathi nekhandlayo.Isifanekiso se-RNA singatholakala ngemizuzu engu-7 nje.I-5×Direct RT Mix kanye ne-2×Direct qPCR Mix-SYBR ama-reagents anikezwe ikhithi angathola ngokushesha nangempumelelo imiphumela ye-PCR yobuningi besikhathi sangempela.

I-5×Direct RT Mix kanye ne-2×Direct qPCR Mix-SYBR inokubekezelela okuqinile kwe-inhibitor, futhi i-lysate yamasampuli ingasetshenziswa njengesifanekiso se-RT-qPCR ngokuqondile.Le khithi iqukethe i-RNA high-affinity Foregene reverse transcriptase, kanye ne-Hot D-Taq DNA polymerase, dNTPs, MgCl₂, isigcinalwazi sokusabela, i-PCR optimizer nesiqinisi.

Imininingwane

200×20μl Rxns, 1000×20μl Rxns

Izingxenye zekhithi

Izici&izinzuzo

■ Kulula futhi kusebenza ngempumelelo : Ngobuchwepheshe be-Cell Direct RT, amasampula e-RNA angatholwa ngemizuzu engu-7 nje.

■ Isidingo sesampula sincane, siphansi njengoba amaseli ayi-10 angahlolwa.

■ Ukuphuma okuphezulu: ikwazi ukubona ngokushesha i-RNA kumaseli akhiwe kumapuleti angu-384, 96, 24, 12, 6-wemithombo.

■ I-DNA Eraser ingasusa ngokushesha ama-genome akhululiwe, inciphise kakhulu umthelela emiphumeleni yokuhlola elandelayo.

■ Isistimu ye-RT ethuthukisiwe kanye ne-qPCR yenza ukuloba okuhlanekezela okuzinyathelo ezimbili kwe-RT-PCR kusebenze kahle futhi i-PCR icace kakhulu, futhi imelane kakhulu ne-RT-qPCR reaction inhibitors.

Isicelo sekhithi

Ububanzi bokusebenza: amaseli akhulisiwe.

- I-RNA ekhishwe ngesampula ye-lysis: isebenza kuphela kusifanekiso se-RT-qPCR sale kit.

- Ikhithi ingasetshenziselwa lezi zinhloso ezilandelayo: ukuhlaziywa kwesisho sofuzo, ukuqinisekiswa komphumela wokuthulisa ufuzo we-siRNA-mediated, ukuhlolwa kwezidakamizwa, njll.

Umdwebo

Isitoreji kanye Nempilo yeshelufu

Ingxenye I yale kit kufanele igcinwe ku-4℃;Ingxenye II kufanele igcinwe ku -20 ℃.

I-Foregene Protease Plus II kufanele igcinwe ku-4℃, ingafrizi ku -20℃.

I-Reagent 2×Direct qPCR Mix-SYBR kufanele igcinwe ku -20℃ ebumnyameni;uma isetshenziswa njalo, ingabuye igcinwe ku-4℃ ukuze igcinwe isikhathi esifushane (isetshenziswe phakathi nezinsuku eziyi-10) .Enesimo sengqondo esihle nesinenqubekela phambili ekuthatheni kwakhe amakhasimende, ibhizinisi lethu lihlala lithuthukisa into yethu ngendlela enhle kakhulu ukuze sanelise izifiso zamakhasimende futhi ligxile kakhulu ekuphepheni, ekuthembekeni, ezidingweni zemvelo, nasekusungulweni kwe-Accounting Bead Machine ye-PC ye-China, i-China i-Nuclear-CER. Ngethemba ukuthi siyakhula kanye namathemba ethu kuyo yonke imvelo.
Umkhiqizi weIkhithi yokukhipha iDNA yaseChina, Ikhithi yokukhipha i-Nucleic Acid, Sinamathela kuklayenti 1st, izinga eliphezulu 1st, ukuthuthukiswa okuqhubekayo, ukusizakala kanye nemigomo yokuwina-win.Uma sibambisana nekhasimende, siletha abathengi abanekhwalithi ephezulu kakhulu yesevisi.Sisungule ubudlelwano obuhle bebhizinisi sisebenzisa umthengi waseZimbabwe ngaphakathi kwebhizinisi, sesizakhele igama nesithunzi.Ngesikhathi esifanayo, ngenhliziyo yonke wamukele amathemba amasha namadala enkampanini yethu ukuya futhi axoxisane namabhizinisi amancane.