Inkampani yethu ithembisa bonke abasebenzisi bemikhiqizo nezisombululo ezisezingeni eliphezulu kanye nosizo olwanelisa kakhulu lwangemva kokuthengisa.Samukela ngemfudumalo abathengi bethu abavamile nabasha ukuthi bahlanganyele nathi ku-High Quality China Rna Purification Kit (Ikholomu Ye-Spin), Sisebenzisa inhloso yaphakade “yokuthuthukiswa kwekhwalithi eqhubekayo, ukwaneliseka kwamakhasimende”, siyaqiniseka ukuthi into yethu enhle kakhulu ivikelekile futhi inomthwalo wemfanelo futhi imikhiqizo yethu nezisombululo zithengiswa kakhulu ekhaya lakho naphesheya kwezilwandle.
Ifemu yethu ithembisa bonke abasebenzisi bemikhiqizo nezisombululo ezisezingeni eliphezulu kanye nosizo olwanelisa kakhulu lwangemva kokuthengisa.Samukela ngokufudumele abathengi bethu abavamile nabasha ukuthi basijoyineChina Rna Isizinda, Dna Test, Yamukela noma yimiphi imibuzo yakho kanye nokukhathazeka ngezimpahla zethu.Sibheke ngabomvu ukusungula ubudlelwano bebhizinisi besikhathi eside nawe maduze nje.Xhumana nathi namuhla.Singuzakwethu wokuqala webhizinisi lakho!

Incazelo

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Le khithi isebenzisa ikholomu yokuphotha kanye nefomula eyakhiwe yi-Foregene, engakhipha ngempumelelo i-RNA ephezulu nekhwalithi ephezulu kumaseli akhuliswe ngamapuleti emithombo engu-96, 24, 12, kanye ne-6.

Ikhithi inikeza Ikholomu Yokuhlanza I-DNA esebenza kahle, engahlukanisa kalula i-supernatant ne-cell lysate, ibophe futhi isuse i-genomic DNA.Ukusebenza kulula futhi konga isikhathi.

Ikholomu ye-RNA kuphela ingahlanganisa ngempumelelo i-RNA ngefomula ehlukile.Inani elikhulu lamasampula lingacutshungulwa kanyekanye.

Izingxenye zekhithi

*Sicela ugqoke amagilavu ​​futhi uthathe izinyathelo zokuvikela ngesikhathi sokusebenza njengoba i-Buffer cRL1 ne-Buffer RW1 iqukethe usawoti we-chaotropic ocasulayo.

Izici&izinzuzo

■ Yonke inqubo iqhutshwa ekamelweni lokushisa (15-25 ℃), ngaphandle kokugeza kweqhwa kanye nokushisa okuphansi kwe-centrifugation.
■ Ikhithi yonke ayinayo i-RNase-Free, asikho isidingo sokukhathazeka ngokuwohloka kwe-RNA.
■ Ikholomu Yokuhlanza I-DNA ibopha ngokukhethekile i-DNA, ukuze ikhithi ikwazi ukususa ukungcola kwe-DNA ye-genomic ngaphandle kokwengeza i-DNase eyengeziwe.
■ Isivuno esikhulu se-RNA: Ikholomu ye-RNA kuphela kanye nefomula eyingqayizivele ingahlanza i-RNA ngokuphumelelayo.
■ Isivinini esisheshayo: kulula ukusebenza futhi singaqedwa ngemizuzu eyi-11.
■ Ukuphepha: Asikho i-reagent ephilayo edingekayo.
■ Ikhwalithi ephezulu: I-RNA ehlanziwe imsulwa kakhulu, ayinamaprotheni nokunye ukungcola, futhi ingahlangabezana nokuhlola okuhlukahlukene okulandelayo.

Isicelo sekhithi

Ilungele ukukhishwa nokuhlanzwa kwe-RNA ephelele kumaseli akhulisiwe kumapuleti angama-96, 24, 12, kanye namapuleti angu-6.

Ukugeleza komsebenzi

Umdwebo

Umdwebo webhethri yejeli ye-agarose ye-Cell Total RNA Isolation Kit iphathe izinombolo ezingenhla ezihlukene zamaseli, ukukhishwa kwevolumu engu-20μl, thatha u-2μl isamba esihlanziwe se-RNA 1%.

Isitoreji nempilo yeshelufu

Ikhithi ingagcinwa izinyanga eziyi-12 ekamelweni lokushisa (15-25 ℃) noma 2-8 ℃ isikhathi eside (izinyanga ezingama-24).

I-Buffer cRL1 ingagcinwa ku-4 ℃ inyanga engu-1 ngemva kokwengeza i-2-hydroxy-1-ethanethiol(ongakukhetha).

Inkampani yethu ithembisa bonke abasebenzisi bemikhiqizo nezisombululo ezisezingeni eliphezulu kanye nosizo olwanelisa kakhulu lwangemva kokuthengisa.Samukela ngemfudumalo abathengi bethu abavamile nabasha ukuthi bahlanganyele nathi ku-High Quality China Rna Purification Kit (Ikholomu Ye-Spin), Sisebenzisa inhloso yaphakade “yokuthuthukiswa kwekhwalithi eqhubekayo, ukwaneliseka kwamakhasimende”, siyaqiniseka ukuthi into yethu enhle kakhulu ivikelekile futhi inomthwalo wemfanelo futhi imikhiqizo yethu nezisombululo zithengiswa kakhulu ekhaya lakho naphesheya kwezilwandle.
Ikhwalithi ephezuluChina Rna Isizinda, Ukuhlolwa Kwe-DNA, Siyakwamukela noma yimiphi imibuzo yakho nokukukhathazayo ngezimpahla zethu.Sibheke ngabomvu ukusungula ubudlelwano bebhizinisi besikhathi eside nawe maduze nje.Xhumana nathi namuhla.Singuzakwethu wokuqala webhizinisi lakho!